Transmembrane helices 3 and 4 are involved in substrate recognition by the Na+/dicarboxylate cotransporter, NaDC1

Biochemistry

Volumn 45, Issue 7, 2006, Pages 2302-2310

  Oshiro, Naomi ^a   King, Steven C ^b   Pajor, Ana M ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

^b OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; CATALYSTS; ELECTRODES; POSITIVE IONS; REACTION KINETICS; SODIUM; SUBSTITUTION REACTIONS;

CHIMERAS; TRANSMEMBRANE HELICES (TM); TRANSPORT SPECIFICITY RATIO (TSR); VOLTAGE CLAMP; XENOPUS OOCYTES;

BIOLOGICAL MEMBRANES;

AMINO ACID; CARBON 14; COTRANSPORTER; GLUTARIC ACID; SODIUM DICARBOXYLATE COTRANSPORTER; SUCCINIC ACID; TRITIUM; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CHIMERA; CITRIC ACID CYCLE; CONTROLLED STUDY; ION CURRENT; MOLECULAR INTERACTION; MOLECULAR RECOGNITION; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DOMAIN; RABBIT; VOLTAGE CLAMP; XENOPUS;

ANIMALS; DICARBOXYLIC ACID TRANSPORTERS; GLUTARATES; MICE; MUTAGENESIS, SITE-DIRECTED; MUTANT CHIMERIC PROTEINS; OOCYTES; ORGANIC ANION TRANSPORTERS, SODIUM-DEPENDENT; PATCH-CLAMP TECHNIQUES; PROTEIN STRUCTURE, SECONDARY; RABBITS; SYMPORTERS; XENOPUS LAEVIS;

ORYCTOLAGUS CUNICULUS;

EID: 33144463939     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi052328g     Document Type: Article

References (34)

1. Pajor, A. M. (2006) Molecular properties of the SLC13 family of dicarboxylate and sulfate transporters, Pflugers Arch. 451, 597-605.
2. Pajor, A. M. (2000) Molecular properties of sodium/dicarboxylate cotransporters, J. Membr. Biol. 175, 1-8.
3. Dantzler, W. H. and Evans, K. K. (1996) Effect of αKG in lumen on PAH transport by isolated perfused proximal tubules, Am. J. Physiol. 271, F521-F526.
4. Pak, C. Y. C. (1991) Etiology and treatment of urolithiasis, Am. J. Kidney Diseases 18, 624-637.
5. Rogina, B., Reenan, R. A., Nilsen, S. P., and Helfand, S. L. (2000) Extended life-span conferred by cotransporter gene mutations in Drosophila, Science 290, 2137-2140.
6. Fei, Y. J., Inoue, K., and Ganapathy, V. (2003) Structural and functional characteristics of two sodium-coupled dicarboxylate transporters (ceNaDC1 and ceNaDC2) from Caenorhabditis elegans and their relevance to life span, J. Biol. Chem. 278, 6136-6144.
7. +-dicarboxylate cotransporter using antifusion protein antibodies, Am. J. Physiol. 271, C1808-C1816.
8. +/dicarboxylate cotransporter: the N-terminus and hydrophilic loop 4 are located intracellularly, Biochim. Biophys. Acta 1511, 80-89.
9. +/dicarboxylate cotransporter, Biochemistry 38, 6151-6156.
10. +/dicarboxylate co-transporter NaDC-1, Biochem. J. 350, 677-683.
11. +/sulfate cotransporters is located in the carboxy-terminal portion of the protein, Biochim. Biophys. Acta 1370, 98-106.
12. +/dicarboxylate cotransporter, J. Biol. Chem. 273, 18923-18929.
13. Pajor, A. M., and Sun, N. N. (2000) Molecular cloning, chromosomal organization, and functional characterization of a sodium-dicarboxylate cotransporter from mouse kidney, Am. J. Physiol. Renal Physiol. 279, F482-F490.
14. Burckhardt, B. C., Lorenz, J., Kobbe, C., and Burckhardt, G. (2005) Substrate specificity of the human renal sodium dicarboxylate cotransporter, hNaDC-3, under voltage-clamp conditions, Am. J. Physiol. Renal Physiol. 288, F792-F799.
15. Kunkel, T. A. (1985) Rapid and efficient site-specific mutagenesis without phenotypic selection, Proc. Natl. Acad. Sci. U.S.A. 82, 488-492.
16. Pajor, A. M. (1995) Sequence and functional characterization of a renal sodium/dicarboxylate cotransporter, J. Biol. Chem. 270, 5779-5785.
17. +/dicarboxylate cotransporter under voltage clamp conditions, Am. J. Physiol. 279, F54-F64.
18. King, S. C. (2004) The "Transport Specificity Ratio": a structure-function tool to search the protein fold for loci that control transition-state stability in membrane transport catalysis, BMC Biochem. 5, 16.
19. Wells, J. A. (1990) Additivity of mutational effects in proteins, Biochemistry 29, 8509-8517.
20. +-dicarboxylate cotransporters, NaDC-1 and hNaDC-1, Am. J. Physiol. 271, F1093-F1099.
21. Baric, I., Wagner, L., Feyh, P., Liesert, M., Buckel, W., and Hoffmann, G. F. (1999) Sensitivity and specificity of free and total glutaric acid and 3-hydroxyglutaric acid measurements by stable-isotope dilution assays for the diagnosis of glutaric aciduria type I, J. Inherited Metab. Dis. 22, 867-881.
22. Kushnir, M. M., Komaromy-Hiller, G., Shushan, B., Urry, F. M., and Roberts, W. L. (2001) Analysis of dicarboxylic acids by tandem mass spectrometry. High-throughput quantitative measurement of methylmalonic acid in serum, plasma, and urine, Clin. Chem. 47, 1993-2002.
23. +/dicarboxylate cotransporter are conformationally sensitive residues, Biochemistry 41, 1083-1090.
24. +/dicarboxylate co-transporter, J. Biol. Chem. 276, 29961-29968.
25. +/dicarboxylate co-transporter, J. Biol. Chem. 280, 18728-18735.
26. King, S. C., Hu, L. A., and Pugh, A. (2003) Induction of substrate specificity shifts by placement of alanine insertions within the consensus amphipathic region of the Escherichia coli GABA (γ-aminobutyric acid) transporter encoded by GabP, Biochem. J. 376, 645-653.
27. Itokawa, M., Lin, Z., Cai, N. S., Wu, C., Kitayama, S., Wang, J. B., and Uhl, G. R. (2000) Dopamine transporter transmembrane domain polar mutants: ΔG and ΔΔG values implicate regions important for transporter functions, Mol. Pharmacol. 57, 1093-1103.
28. Kasahara, T., and Kasahara, M. (2003) Transmembrane segments 1, 5, 7 and 8 are required for high-affinity glucose transport by Saccharomyces cerevisiae Hxt2 transporter, Biochem. J. 372, 247-252.
29. Buchs, A. E., Sasson, S., Joost, H. G., and Cerasi, E. (1998) Characterization of GLUT5 domains responsible for fructose transport, Endocrinology 139, 827-831.
30. Wu, L., Fritz, J. D., and Powers, A. C. (1998) Different functional domains of GLUT2 glucose transporter are required for glucose affinity and substrate specificity, Endocrinology 139, 4205-4212.
31. Manolescu, A., Salas-Burgos, A. M., Fischbarg, J., and Cheeseman, C. I. (2005) Identification of a hydrophobic residue as a key determinant of fructose transport by the facultative hexose transporter SLC2A7(GLUT7), J. Biol. Chem. 280, 42978-42983.
32. --dependent neurotransmitter transporters, Nature 437, 215-223.
33. Abramson, J., Smirnova, I., Kasho, V., Verner, G., Kaback, H. R., and Iwata, S. (2003) Structure and mechanism of the lactose permease of Escherichia coli, Science 301, 610-615.
34. Klingenberg, M. (2005) Ligand-protein interaction in biomembrane carriers. The induced transition fit of transport catalysis, Biochemistry 44, 8563-8570.