Assessment of cell viability in intact glandular tissue in Chironomus ramosus using dye-exclusion and colorimetric assays

Cytotechnology

Volumn 49, Issue 1, 2005, Pages 59-65

  Nath, B B ^a   Babrekar, A A ^a   Parthasarathy, B ^a  

^a UNIVERSITY OF PUNE   (India)

Author keywords

Cell viability; Chironomus; Dye exclusion assay; MTT; Nigrosin; Salivary gland; Tissue viability; Vital dye

Indexed keywords

DYE; TETRAZOLIUM;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BIOCHEMISTRY; CALORIMETRY; CELL ASSAY; CELL SUSPENSION; CELL VIABILITY; CHIRONOMIDAE; CHIRONOMUS RAMOSUS; NONHUMAN; QUANTITATIVE ANALYSIS; RELIABILITY; SALIVARY GLAND; STANDARDIZATION; TISSUE CULTURE;

ANIMALIA; CHIRONOMIDAE; CHIRONOMUS RAMOSUS;

EID: 32944471723     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10616-005-4538-9     Document Type: Article

References (24)

1. P. Armitage P.S. Cranston L.C.V. Pinder 1994 Chironomidae: Biology & Ecology of Non-biting Midges Chapman & Hall London
2. H.L. Bank 1987 Assessment of islet cell viability using fluorescent dyes Diabetologia 30 812 816
3. T. Bernas J. Dubrucki 2002 Mitochondrial and nonmitochondrial reduction of MTT: interaction of MTT with TMRE, JC-1 and NAO mitochondrial fluorescent probes Cytometry 47 236 242
4. A.O. De Coste M.C. De Assis E.A. Marques M.C. Plotkowski 1999 Comparative analysis of three methods to assess viability of mammalian cells in culture Biocell 23 65 72
5. A.G. Doukes T.J. Flotte 1996 Physical characteristics and biological effects of laser-induced stress waves Ultrasound Med. Biol. 22 151 64
6. T.A. Drixler M.J. Vogten E.D. Ritchie V.T.J. Van M.F. Gebbink E.E. Voest R.I.H. Borel 2002 Liver regeneration is an angiogenesis-associated phenomenon Ann. Surg. 236 703 712
7. R.I. Freshney 2000 Culture of Animal Cells : A Manual of Basic Technique 4 Wiley & Liss Inc New York
8. U. Grossbach 1977 The salivary gland of Chironomus (Diptera): a model system for the study of cell differentiation W. Beermann Results and Problems Cell in Differentiation Vol. 8 Springer-Verlag Berlin 147 196
9. K.H. Jones J.A. Senft 1985 An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide J. Histochem. Cytochem. 33 77 79
10. J.P. Kaltenbach M.H. Kaltenbach W.B. Lyons 1958 Nigrosin as a dye for differentiating live and dead ascites cells Exp. Cell. Res. 15 112 117
11. A.W. Krause W.W. Carley W.W. Webb 1984 Fluorescent erythrosin B is preferable to trypan blue as a vital exclusion dye for mammalian cells in monolayer culture J. Histochem. Cytochem. 32 1084 1090
12. D.T. Loo J.R. Rillema 1998 Measurement of cell death Methods Cell Biol. 57 251 264
13. T.L. Means D.H. Geroski A. Hadley M.J. Lym H.F. Edelhauser 1995 Viability of human corneal epithelium following optisol-GS storage Arch. Ophthalmol. 113 805 809
14. T. Mosmann 1983 Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays J. Immunol. Methods 65 55 63
15. B.B. Nath N.N. Godbole 1998 Technique for mass rearing of Indian Chironomus species (DipteraNematoceraChironomidae) Studia Dipterologica 5 187 193
16. B.B. Nath S.C. Lakhotia 1989 Heat shock response and the effect of developmental stage and tissue type on heat shock protein synthesis Genome 32 461 475
17. P. Olinga K. Groen I.H. Hof R.D. Kanter H.J. Koster W.R. Leeman A.A.J.J.L. Rutten K.V. Twillert G.M.N. Groothuis 1997 Comparison of five incubation systems for rat liver slices using functional and viability parameters J. Pharmacol. Toxicol. Methods 38 59 69
18. T.L. Riss R.A. Moravec 1992 Comparison of MTT, XTT, and a novel tetrazolium compound MTS for in vitro proliferation and chemosensitivity assays Mol. Biol. Cell 3 suppl. 184
19. V. Sorsa 1988 Polytene Chromosomes in Genetic Research Ellis Horwood Ltd Chichester
20. M.G. Stevens S.C. Olsen 1993 Comparative analysis of using MTT and XTT in colorimetric assays for quantitating bovine neutrophil bactericidal activity J. Immunol. Methods 157 225 231
21. H. Tada O. Shiho K. Kuroshima M. Koyama K. Tsukamoto 1986 An improved colorimetric assay for interleukin 2 J. Immunol. Methods 93 157 165
22. A.S. Tan M.V. Berridge 2000 Superoxide produced by activated neutrophils efficiently reduces the tetrazolium salt, WST-1 to produce a soluble formazan: a simple colorimetric assay for measuring respiratory burst activation and for screening antiinflammatory agents J. Immunol. Methods 238 59 68
23. H.N. Traore D. Meyer 2001 Comparing qualitative and quantitative spectroscopic techniques for the detection of the effect of direct iron loading of mammalian cell cultures Methods Cell Sci. 23 175 184
24. H. Yamane K. Konishi H. Iguchi T. Nakagawa S. Shibata M. Takayama K. Nishimura K. Sunami Y. Nakai 1998 Assessment of hair cell death using the dye extrusion method Acta Otolaryngol. Suppl. 538 7 11