Superoxide produced by activated neutrophils efficiently reduces the tetrazolium salt, WST-1 to produce a soluble formazan: A simple colorimetric assay for measuring respiratory burst activation and for screening anti-inflammatory agents

Journal of Immunological Methods

Volumn 238, Issue 1-2, 2000, Pages 59-68

  Tan, An S ^a   Berridge, Michael V ^a  

^a MALAGHAN INSTITUTE OF MEDICAL RESEARCH   (New Zealand)

Author keywords

Anti inflammatory; NADPH oxidase; Respiratory burst; Superoxide; Tetrazolium; WST 1

Indexed keywords

2 (4 IODOPHENYL) 3 (4 NITROPHENYL) 5 (2,4 DISULFOPHENYL) 2H TETRAZOLIUM SODIUM; AZIDE; CATALASE; CYTOCHROME C OXIDASE; DIPHENYLENEIODINIUM; FORMYLMETHIONYLLEUCYLPHENYLALANINE; PHORBOL 13 ACETATE 12 MYRISTATE; RESINIFERATOXIN; SUPEROXIDE; SUPEROXIDE DISMUTASE; TETRAZOLIUM; UNCLASSIFIED DRUG; WST 1;

ANTIINFLAMMATORY ACTIVITY; ARTICLE; COLORIMETRY; CONTROLLED STUDY; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; LEUKOCYTE ACTIVATION; NORMAL HUMAN; PRIORITY JOURNAL; RESPIRATORY BURST; SCREENING; TECHNIQUE;

ANTI-INFLAMMATORY AGENTS; CELLS, CULTURED; COLORIMETRY; CYTOCHROME C GROUP; DRUG EVALUATION, PRECLINICAL; FORMAZANS; HUMANS; MULTIENZYME COMPLEXES; NADH, NADPH OXIDOREDUCTASES; NADPH OXIDASE; NEUTROPHIL ACTIVATION; NEUTROPHILS; RESPIRATORY BURST; SUPEROXIDE DISMUTASE; SUPEROXIDES; TETRAZOLIUM SALTS;

EID: 0034697387     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(00)00156-3     Document Type: Article

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