Homology-model-guided site-specific mutagenesis reveals the mechanisms of substrate binding and product-regulation of adenosine kinase from Leishmania donovani

Biochemical Journal

Volumn 394, Issue 1, 2006, Pages 35-42

  Datta, Rupak ^a   Das, Ishita ^a,b   Sen, Banibrata ^a   Chakraborty, Anutosh ^a,c   Adak, Subrata ^a   Mandal, Chhabinath ^a   Datta, Alok K ^a  

^a INDIAN INSTITUTE OF CHEMICAL BIOLOGY   (India)

^b UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^c JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Adenosine kinase; AMP regulation; Homology modelling; Leishmania donovani

Indexed keywords

CATALYSIS; HYDROGEN BONDS; MUTAGENESIS; PROTEINS; SUBSTRATES;

ADENOSINE KINASE; AMP REGULATION; HOMOLOGY MODELLING; LEISHMANIA DONOVANI;

ENZYMES;

ADENOSINE KINASE; ARGININE; ASPARTIC ACID;

ARTICLE; ENZYME ACTIVITY; LEISHMANIA DONOVANI; MODEL; NEGATIVE FEEDBACK; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; SPECTROSCOPY; STEADY STATE; STRUCTURAL HOMOLOGY; STRUCTURE ANALYSIS;

ADENOSINE KINASE; ADENOSINE MONOPHOSPHATE; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; BINDING SITES; KINETICS; LEISHMANIA DONOVANI; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY;

LEISHMANIA DONOVANI;

EID: 32944465100     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20051513     Document Type: Article

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