Mutational analysis of the active-site residues crucial for catalytic activity of adenosine kinase from Leishmania donovani

Biochemical Journal

Volumn 387, Issue 3, 2005, Pages 591-600

  Datta, Rupak ^a   Das, Ishita ^a,b   Sen, Banibrata ^a   Chakraborty, Anutosh ^a,c   Adak, Subrata ^a   Mandal, Chhabinath ^a   Datta, Alok K ^a  

^a INDIAN INSTITUTE OF CHEMICAL BIOLOGY   (India)

^b UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^c JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Active site; Adenosine kinase; Leishmania donovani; Purine salvage; Ribokinase; Site directed mutagenesis

Indexed keywords

AMINO ACIDS; CATALYSIS; FREE ENERGY; MATHEMATICAL MODELS; MUTAGENESIS; REDUCTION;

AMINO ACID RESIDUE; LEISHMANIA DONOVANI ADENOSINE KINASE (LDADK); MUTANTS; MUTATIONAL ANALYSIS;

ENZYMES;

ADENOSINE; ADENOSINE KINASE; ARGININE; ASPARTIC ACID; GLYCINE; PHENYLGLYOXAL; PHOSPHATE;

ALPHA HELIX; AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME INACTIVATION; ENZYME KINETICS; ENZYME PURIFICATION; LEISHMANIA DONOVANI; MUTANT; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS;

ADENOSINE KINASE; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; BINDING SITES; CIRCULAR DICHROISM; KINETICS; LEISHMANIA DONOVANI; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN CONFORMATION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID;

LEISHMANIA DONOVANI;

EID: 18844452613     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20041733     Document Type: Article

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