Coenzyme site-directed mutants of photosynthetic A4-GAPDH show selectively reduced NADPH-dependent catalysis, similar to regulatory AB-GAPDH inhibited by oxidized thioredoxin

Journal of Molecular Biology

Volumn 340, Issue 5, 2004, Pages 1025-1037

  Sparla, Francesca ^a   Fermani, Simona ^a   Falini, Giuseppe ^a   Zaffagnini, Mirko ^a   Ripamonti, Alberto ^a   Sabatino, Piera ^a   Pupillo, Paolo ^a   Trost, Paolo ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

BPGA, 1,3 bisphosphoglycerate; enzyme structure; GAPDH, glyceraldehyde 3 phosphate dehydrogenase (EC 1.2.1.13); light regulation; NADP; photosynthetic glyceraldehyde 3 phosphate dehydrogenase; site specific mutants

Indexed keywords

ALANINE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; MUTANT PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; PROTEIN SUBUNIT; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; REGULATOR PROTEIN; SERINE; TETRAMER; THIOREDOXIN; THREONINE;

ARTICLE; CATALYSIS; CHLOROPLAST; COENZYME; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; ENZYME REGULATION; ENZYME STRUCTURE; ESCHERICHIA COLI; METABOLITE; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; SITE DIRECTED MUTAGENESIS; SPINACH; STEADY STATE; WILD TYPE;

EMBRYOPHYTA; ESCHERICHIA COLI; FELIS CATUS; SPINACIA OLERACEA;

EID: 3242801382     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2004.06.005     Document Type: Article

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