Crystal structure of the non-regulatory A4 isoform of spinach chloroplast glyceraldehyde-3-phosphate dehydrogenase complexed with NADP

Journal of Molecular Biology

Volumn 314, Issue 3, 2001, Pages 527-542

  Fermani, Simona ^a   Ripamonti, Alberto ^a   Sabatino, Piera ^a   Zanotti, Giuseppe ^b   Scagliarini, Sandra ^a   Sparla, Francesca ^a   Trost, Paolo ^a   Pupillo, Paolo ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b UNIVERSITY OF PADOVA   (Italy)

Author keywords

Cofactor selectivity; Crystal structure; NADP; Photosynthetic GAPDH; Spinach chloroplasts

Indexed keywords

ADENOSINE 2' PHOSPHATE; CYSTEINE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; ISOPROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; NUCLEOTIDE; THREONINE;

ARTICLE; CHLOROPLAST; COMPLEX FORMATION; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME PURIFICATION; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; HYDROGEN BOND; NONHUMAN; OPTICAL RESOLUTION; PHOTOSYNTHESIS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FAMILY; PROTEIN FOLDING; SPECIES DIFFERENCE; SPINACH;

ANIMALIA; EUKARYOTA; SPINACIA OLERACEA;

EID: 0035976784     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.5172     Document Type: Article

References (58)

1. The chimeric nature of nuclear genomes and the antiquity of introns as demonstrated by the GAPDH gene system
2. Glyceraldehyde-3-phosphate dehydrogenase gene diversity in eubacteria and eukaryotes: Evidence for intra- and inter-kingdom gene transfer
3. Subunit structure of three glyceraldehyde-3-phosphate dehydrogenases of some flowering plants
4. Subunit structure of higher plant glyceraldehyde-3-phosphate dehydrogenase
5. Chloroplast glyceraldehyde-3-phosphate dehydrogenase (NADP): Amino acid sequence of the subunits from isoenzyme I and structural relationship with isoenzyme II
6. The non-regulatory isoform of NADP(H)-glyceraldehyde-3-phosphate dehydrogenase from spinach chloroplasts
7. Evolutionary origin of cryptomonad microalgae: Two novel chloroplast/cytosol-specific GAPDH genes as potential markers of ancestral endosymbiont and host cell components
8. The phylogeny of glyceraldehyde-3-phosphate dehydrogenase indicates lateral gene transfer from cryptomonads to dinoflagellates
9. Compartment-specific isoforms of TPI and GAPDH are imported into diatom mitochondrial as a fusion protein: Evidence in favour of a mitochondrial origin of the eukaryotic glycolytic pathway
10. Primary structure of D-glyceraldehyde-3-phosphate dehydrogenase from the thermophilic archaebacterium Methanothermus fervidus
11. The reversible depolymerization of spinach chloroplast glyceraldehyde-3-phosphate dehydrogenase. Interaction with nucleotides and dithiothreitol
12. Activation of spinach chloroplast glyceraldehyde-3-phosphate dehydrogenase: Effect of glycerate 1,3-bisphosphate
13. Reductive modification and non-reductive activation of spinach chloroplast NADP-glyceraldehyde-3-phosphate dehydrogenase
14. Light activation and molecular mass changes of NAD(P) glyceraldehyde-3-phosphate dehydrogenases of spinach and maize leaves
15. 4 complex is mediated by the B-subunit carboxy terminus
16. Expression and characterization of pea chloroplastic glyceraldehyde-3-phosphate dehydrogenase composed of only the B-subunit
17. C-terminal truncation of spinach chloroplast NAD(P)-glyceraldehyde-3-phosphate dehydrogenase prevents inactivation and reaggregation
18. Evolutionary conserved light regulation of Calvin cycle activity by NAPDH-mediated reversible phosphoribulokinase/CPl2/ glyceraldehyde-3-phosphate-dehydrogenase complex dissociation
19. Limited proteolysis of chloroplast glyceraldehyde-3-phosphate dehydro enase (NADP) from Spinaca oleracea
20. Twinning in crystals of human skeletal muscle D-glyceraldehyde-3-phosphate dehydrogenase
21. Studies of asymmetry in the three-dimensional structure of lobster D-glyceraldehyde-3-phosphate dehydrogenase
22. Structure of the holo-glyceraldehyde-3-phosphate dehydrogenase from Palinurus versicolor refined at 2.0 Å resolution
23. Refined 3.2 Å resolution structure of glycosomal glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma brucei brucei
24. Trypanosoma cruzi glycosomal glyceraldehyde-3-phosphate dehydrogenase: Structure, catalytic mechanism and targeted inhibitor design
25. Crystal structure of glycosomal glyceraldehyde-3-phosphate dehydrogenase from Leishmania mexicana: Implications for structure-based drug design and a new position for the inorganic phosphate binding site
26. Comparison of the structures of wild-type and a N313T mutant of Escherichia coli glyceraldehyde-3-phosphate dehydrogenases: Implication for NAD binding and cooperativity
27. Sequence and structure of D-glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus
28. Structure of the holo-glyceraldehydde-3-phosphate dehydrogenase from Bacillus stearothermophilus at 1.8 Å of resolution
29. The crystal structure of holo-glyceraldehyde-3-phosphate dehydrogenase from hyperthermophilic bacterium Thermotoga maritima at 2.5 Å resolution
30. Determinants of enzyme thermostability observed in the molecular structure of Thermus aquaticus D-glyceraldehyde-3-phosphate dehydrogenase at 2.5 Å resolution
31. Crystal structure of the glyceraldehyde-3-phosphate dehydrogenase from hyperthermophilic archeon Sulfolobus solfataricus
32. + at 2.1 Å of resolution
33. Molecular symmetry of glyceraldehyde-3-phosphate dehydrogenase from Bacillus coagulans
34. Crystallization and preliminary crystallographic analysis of glyceraldehyde-3-phosphate dehydrogenase from Saccharomyces cerevisiae (baker's yeast)
35. The CCP4 suite: Programs for protein crystallography
36. PROCHECK: A program to check the stereochemical quality of protein structures
37. +
38. Evolutionary and structural relationships among dehydrogenases
39. Recognition of different nucleotide-binding sites in primary structures using a property-pattern approach
40. The interpretation of protein structures; estimation of accessibility
41. Glyceraldehyde-3-phosphate dehydrogenase in photosynthetic tissues: Kinetic evidence for competitivity between NADP and NAD
42. Purification and properties of a nicotinamide adenine phosphate requiring glyceraldehyde phosphate dehydrogenase from spinach leaves
43. NADP-dependent enzymes. I. Conserved stereochemistry of cofactor binding
44. Crystallographic study of coenzyme, coenzyme analogue and substrate binding in 6-phosphogluconate dehydrogenase: Implication for NADP specificity and the enzyme mechanism
45. Structure of trypanothione reductase from Crithidia fasciculata at 2.6 Å resolution; enzyme-NADP interaction at 2.8 Å resolution
46. The three-dimensional structure of a NAD(P)H:quinone reductase, a flavoprotein involved in cancer chemoprotection and chemoterapy: Mechanism of the two-electron reduction
47. Effects of substitution of Thr63 by alanine on the structure and function of Lactobacillus casei dihydrofolate reductase
48. Role of Arg231 and Tyr284 of Thermus thermophilus isocitrate dehydrogenase in the coenzyme specificity
49. Site-directed mutagenesis of a serine residue in cynnamyl alcohol dehydrogenase, a plant NADPH-dependent dehydrogenase, affects the specificity for the coenzyme
50. Determinants of coenzyme specificity in glyceraldehyde-3-phosphate dehydrogenase: Role of the acidic residue in the fingerprint region of the nucleotide binding fold
51. Crystallization and preliminary X-ray study of chloroplast glyceraldehyde-3-phosphate dehydrogenase
52. Processing of X-ray diffraction data collected in oscillation mode
53. Solvent content of protein crystals
54. Automated refinement of protein models
55. Improved methods for building models in electron density maps and the location of errors in these models
56. Crystallography & NMR System (CNS): A new software system for macromolecular structure determination
57. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
58. A superposition