Characterization of native and recombinant A4 glyceraldehyde 3-phosphate dehydrogenase: Kinetic evidence for conformation changes upon association with the small protein CP12

European Journal of Biochemistry

Volumn 270, Issue 1, 2003, Pages 129-136

  Graciet, Emmanuelle ^a   Lebreton, Sandrine ^a   Camadro, Jean Michel ^a   Gontero, Brigitte ^a  

^a INSTITUT JACQUES MONOD   (France)

Author keywords

CP12; GAPDH; Kinetics; Overexpression; Purification

Indexed keywords

CYSTEINE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GLYCERIC ACID; GLYCOLYTIC ENZYME; HISTIDINE; PROTEIN; PROTEIN CP12; RECOMBINANT ENZYME; TETRAMER; UNCLASSIFIED DRUG;

ALKALINITY; ARTICLE; CATALYSIS; CHLAMYDOMONAS REINHARDTII; CHLOROPLAST; ENZYMATIC ASSAY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME PURIFICATION; ESCHERICHIA COLI; GLYCOLYSIS; GREEN ALGA; MASS SPECTROMETRY; MICHAELIS MENTEN KINETICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SPINACH;

ANIMALS; BACTERIAL PROTEINS; CHLAMYDOMONAS REINHARDTII; DIMERIZATION; DIPHOSPHOGLYCERIC ACIDS; ESCHERICHIA COLI; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (NADP+)(PHOSPHORYLATING); HYDROGEN-ION CONCENTRATION; KINETICS; MEMBRANE TRANSPORT PROTEINS; NADP; PLANT PROTEINS; PROTEIN CONFORMATION; PROTEIN SUBUNITS; RECOMBINANT PROTEINS;

ALGAE; CHLAMYDOMONAS; CHLAMYDOMONAS REINHARDTII; CHLOROPHYTA; EMBRYOPHYTA; ESCHERICHIA COLI; SPINACIA OLERACEA;

EID: 0037220140     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2003.03372.x     Document Type: Article

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