A protein-protein binding assay using coated microtitre plates: Increased throughput, reproducibility and speed compared to bead-based assays

Journal of Biochemical and Biophysical Methods

Volumn 60, Issue 1, 2004, Pages 49-60

  Craig, Tim J ^a   Ciufo, Leonora F ^a   Morgan, Alan ^a  

^a UNIVERSITY OF LIVERPOOL   (United Kingdom)

Author keywords

Binding assay; GST; Proteomics; Pull down

Indexed keywords

GLUTATHIONE TRANSFERASE; SEPHAROSE; SYNTAXIN;

ARTICLE; BINDING ASSAY; BIOLOGY; CALORIMETRY; ENZYME LINKED IMMUNOSORBENT ASSAY; ISOTHERMAL TITRATION CALORIMETRY; KINETICS; MICROTITER PLATE ASSAY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEOMICS; REPRODUCIBILITY; SCINTILLATION; SURFACE PLASMON RESONANCE; VALIDATION PROCESS;

AUTOMATION; BIOCHEMISTRY; CALORIMETRY; CHROMATOGRAPHY, AFFINITY; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GLUTATHIONE; GLUTATHIONE TRANSFERASE; KINETICS; MEMBRANE PROTEINS; MUNC18 PROTEINS; NERVE TISSUE PROTEINS; PROTEIN BINDING; PROTEIN BIOSYNTHESIS; QA-SNARE PROTEINS; RECOMBINANT PROTEINS; SEPHAROSE; SURFACE PLASMON RESONANCE; TIME FACTORS; VESICULAR TRANSPORT PROTEINS;

EID: 3242727778     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbbm.2004.04.015     Document Type: Article

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