Molecular dynamics simulations of active site mutants of rat liver arginase

Electronic Journal of Biotechnology

Volumn 4, Issue 3, 2001, Pages 65-71

  Canales, Mauricio ^a   Westermeyer, Linda ^b   Carvajal, Nelson ^a  

^a UNIVERSITY OF CONCEPCIÓN   (Chile)

^b UNIVERSIDAD ADVENTISTA DE CHILE   (Chile)

Author keywords

Arginase; Arginine; Molecular dynamics; Optimization

Indexed keywords

ARGINASE; LIVER ENZYME;

ARTICLE; CATALYSIS; CRYSTALLOGRAPHY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; GENETIC VARIABILITY; MOLECULAR DYNAMICS; MUTATION; SIMULATION;

EID: 3242679933     PISSN: 07173458     EISSN: None     Source Type: Journal    
DOI: 10.2225/vol4-issue3-fulltext-6     Document Type: Article

References (12)

1. Aminlari, M. and Vaseghi, T. (1992). Arginase distribution in tissues of domestic animals. Comparative Biochemistry and Physiology B - Biochemistry and Molecular Biology 103:385-389.
2. Hypercube, Inc. HyperChem Computational Chemistry. Publication 100033-01, March 1992.
3. Bewley, M.C.; Jeffery, P.D.; Patchett, M.L.; Kanyo, Z.F. and Baker, E.N. (1999). Crystal structures of Bacillus caldovelox arginase in complex with substrate and inhibitors reveal new insights into activation, inhibition and catalysis in the arginase superfamily. Structure with Folding and Design 7:435-448.
4. Biosym Technologies. Insight II Version 2.3.0 User Guide, Part 1. Biosym, San Diego, USA, 1995.
5. Carvajal, N.; Torres, C.; Uribe, E. and Salas, M. (1995). Interaction of arginase with metal ions: Studies of the enzyme from human liver and comparison with other arginases. Comparative Biochemistry and Physiology B - Biochemistry and Molecular Biology 112:153-159.
6. Carvajal, N.; Olate, J.; Salas M.; Uribe, E.; Lopez, V.; Herrera, P. and Cerpa, J. (1999). Chemical modification and site-directed mutagenesis of human liver arginase: Evidence that the imidazole group of histidine-141 is not involved in substrate binding. Archives of Biochemistry and Biophysics 371:2 202-206.
7. Carvajal, N. and Uribe, E. (1995). Attempted reactivation after inactivation of rat liver arginase by guanidinium chloride. Biochemical Archives 11:95-101.
8. Cavalli, R.L.; Burke, C.J.; Kawamoto, S.; Soprano, D.R. and Ash D.E. (1994). Mutagenesis of rat liver arginase expressed in Escherichia coli: Role of conserved histidines. Biochemistry 33:10652-10657.
9. Chang, Cl.; Lias, J.C. and Kuo, L. (1998). Arginase modulates nitric oxide production in Activated macrophages. American Journal of Physiology - Heart and Circulatory Physiology 274:H342-H348.
10. Kanyo, Z.F.; Scolnick, L.R.; Ash, D.E. and Christianson, D.W. (1996). Structure of a unique binuclear manganese cluster in arginase. Nature 383:554-557.
11. Khangulov, S.V.; Sossong, T.M.; Ash, D.E. and Dismukes G.C. (1998). L-Arginine binding to liver arginase requires proton transfer to gateway residue His 141 and coordination of the guanidinium group to the dimangenese (II,II) center. Biochemistry 37:8539-8550.
12. Perozich, J.; Hempel, J. and Morris, S.M. (1998). Roles of conserved residues in the arginase family. Biochimica et Biophysica Acta 1382:23-37.