Immortalization of human nucleus pulposus cells by a recombinant SV40 adenovirus vector: Establishment of a novel cell line for the study of human nucleus pulposus cells

Spine

Volumn 29, Issue 14, 2004, Pages 1515-1523

  Sakai, Daisuke ^a   Mochida, Joji ^a   Yamamoto, Yukihiro ^a   Toh, Eiren ^a   Iwashina, Tohru ^a   Miyazaki, Takeshi ^a   Inokuchi, Sadaki ^a   Ando, Kiyoshi ^a   Hotta, Tomomitsu ^a  

^a TOKAI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Cell line; Immortalization; Intervertebral disc; Nucleus pulposus cells

Indexed keywords

ADENOVIRUS VECTOR; DNA; PROTEOGLYCAN; VIRUS T ANTIGEN;

ANTIGEN EXPRESSION; ARTICLE; CARCINOGENESIS; CELL CYCLE; CELL FUNCTION; CELL IMMORTALIZATION; CELL LINE; CELL PROLIFERATION; CELL STRUCTURE; CYTOLOGY; DNA SYNTHESIS; GENE EXPRESSION PROFILING; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; INFORMED CONSENT; KARYOTYPING; NUCLEUS PULPOSUS; PRIORITY JOURNAL; PROTEOGLYCAN SYNTHESIS; SIMIAN VIRUS 40;

ADENOVIRUSES, HUMAN; ADULT; ANIMALS; ANTIGENS, POLYOMAVIRUS TRANSFORMING; CELL DIVISION; CELL LINE, TRANSFORMED; CELL TRANSFORMATION, VIRAL; DNA REPLICATION; FEASIBILITY STUDIES; GENE EXPRESSION PROFILING; GENETIC VECTORS; HUMANS; INTERVERTEBRAL DISK; KARYOTYPING; LUMBAR VERTEBRAE; MALE; PROTEOGLYCANS; RABBITS; SIMIAN VIRUS 40;

EID: 3242672324     PISSN: 03622436     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.BRS.0000131419.25265.23     Document Type: Article

References (32)

1. Gruber HE, Hanley EN. Recent advances in disc cell biology. Spine. 2003;28:186-193.
2. Phillips FM, An HS, Kang JD, et al. Biologic treatment for intervertebral disc degeneration. Spine. 2003;28(suppl):99.
3. An HS, Thonar EJ, Masuda K. Biological repair of intervertebral disc. Spine. 2003;28(suppl):86-92.
4. Roughley PJ. Point of view. Spine. 2001;26:1752.
5. Antoniou J, Steffen T, Nelson F, et al. The human intervertebral disc. J Clin Invest. 1996;98:996-1003.
6. Doren KV, Glunzman Y, et al. Efficient transformation of human fibroblasts by adenovirus-Simian Virus 40 recombinants. Mol Cell Biol. 1984;4:1653-1656.
7. Gruber HE, Hanley EN Jr. Human disc cells in monolayer vs 3D culture: cell shape, division and matrix formation. BMC Musculoskelet Disord. 2000;1:1.
8. Maldonado BA, Oegema TR. Initial characterization of the metabolism of intervertebral disc cells encapsulated in microspheres. J Orthop Res. 1992;10:677-690.
9. Chiba K, Andersson GB, Masuda K. A new culture system to study the metabolism of the intervertebral disc in vitro. Spine. 1998;23:1821-1827.
10. Isobe I, Michikawa M, Yanagisawa K. Enhancement of MTT, a tetrazolium salt, exocytosis by amyloid beta-protein and chloroquine in cultured rat astrocytes. Neurosci Lett. 1999;266:129-132.
11. Okuma M, Mochida J, Nishimura K, et al. Reinsertion of stimulated nucleus pulposus cells retards intervertebral disc degeneration: an in vitro and in vivo experimental study. J Orthop Res. 2000;18:988-997.
12. Nomura T, Mochida J, Okuma M, et al. Nucleus pulposus allograft retards intervertebral disc degeneration. Clin Orthop. 2001;389:94-101.
13. Sakai D, Mochida J, Yamamoto Y, et al. Transplantation of mesenchymal stem cells embedded in Atelocollagen gel to the intervertebral disc: a potential therapeutic model for disc degeneration. Biomaterials. 2003;24:3531-3541.
14. Nishida K, Kang JD, Gilbertson LG, et al. 1999 Volvo Award in basic science. Modulation of the biologic activity of the rabbit intervertebral disc by gene therapy: an in vivo study of adenovirus-mediated transfer of the human transforming growth factor β1 encoding gene. Spine. 1999;24:2419-2425.
15. Mochida J, Nishimura K, Nomura T, et al. The importance of preserving disc structure in surgical approaches to lumbar disc herniation. Spine. 1996;21:1556-1564.
16. Oegema TR Jr. Biochemistry of the intervertebral disc. Clin Sports Med. 1993;12:419-439.
17. Buckwalter JA. Aging and degeneration of the human intervertebral disc. Spine. 1995;20:1307-1314.
18. Pearce RH, Grimmer BJ, Adams ME. Degeneration and the chemical composition of the human lumbar intervertebral disc. J Orthop Res. 1987;5:198-205.
19. Nishimura K, Mochida J. Percutaneous reinsertion of the nucleus pulposus. Spine. 1998;23:1531-1539.
20. Inokuchi S, Ueda M, Hashimoto K, et al. Immortalization of human epidermal keratinocytes by the recombinant SV40-adenovirus vector. In Vitro Cell Dev Biol. 1991;27:827-828.
21. Inokuchi S, Handa H, Imai T, et al. Immortalisation of human oesophageal epithelial cells by a recombinant SV40 adenovirus vector. Br J Cancer. 1995;71:819-825.
22. Moon S-H, Gilbertson LG, Nishida K, et al. Human intervertebral disc cells are genetically modifiable by adenovirus-mediated gene transfer: implications for the clinical management of intervertebral disc disorders. Spine. 2000;25:2573-2579.
23. Kim KW, Lim TH, Kim JG, et al. The origin of chondrocytes in the nucleus pulposus and histologic findings associated with the transition of a notochordal nucleus pulposus to a fibrocartilaginous nucleus pulposus in intact rabbit intervertebral discs. Spine. 2003;28:982-990.
24. Gruber HE, Fisher EC Jr, Desai B, et al. Human intervertebral disc cells from the annulus: three-dimensional culture in agarose or alginate and responsiveness to TGF-β1. Exp Cell Res. 1997;235:13-21.
25. Chelberg MK, Banks GM, Geiger DF, et al. Identification of heterogeneous cell populations in normal human intervertebral disc. J Anat. 1995;186:43-53.
26. Spector DL, Goldman RD, Leinwand LA. Cells: A Laboratory Manual, vol. 1. Col Spring Harbor, IL: Cold Spring Harbor, 1998;2.1-4.3.
27. Osada R, Oshima H, Ishihara H, et al. Autocrine/paracrine mechanism of insulin-like growth factor-1 secretion, and the effect of insulin-like growth factor-1 on proteoglycan synthesis in bovine intervertebral discs. J Orthop Res. 1996;14:690-699.
28. Thompson JP, Oegema TR Jr, Bradford DS, et al. Stimulation of mature canine intervertebral disc by growth factors. Spine. 1991;16:253-260.
29. Benoit B, Penfornis P, Thenet-Gauci S, et al. Immortalization of human articular chondrocytes. In Vitro Cell Dev Biol. 1995;31:174-177.
30. Davies BR, Steele IA, Edmondson RJ, et al. Immortalisation of human ovarian surface epithelium with telomerase and temperature-sensitive SV40 large T antigen. Exp Cell Res. 2003;288:390-402.
31. Gruber HE, Johnson TL, Leslie K, et al. Autologous intervertebral disc cell implantation: a model using Psammomys obesus, the sand rat. Spine. 2002;27:1626-1633.
32. Ronjon P, Haydon RC, Cheng H, et al. Potential use of sox9 gene therapy for transfer of the human transforming growth factor β1 encoding gene. Spine. 1999;24:2419-2425.