Purification of potato polyphenol oxidase (PPO) by partitioning in aqueous two-phase system

Biochemical Engineering Journal

Volumn 28, Issue 2, 2006, Pages 161-166

  Vaidya, Bhalchandra K ^a   Suthar, Hitesh K ^a   Kasture, Sangita ^a   Nene, Sanjay ^a  

^a NATIONAL CHEMICAL LABORATORY   (India)

Author keywords

Aqueous two phase systems; Downstream processing; Enzyme purification; Polyphenol oxidase; Protein recovery

Indexed keywords

DERIVATIVES; MOLECULAR WEIGHT; PHASE COMPOSITION; PHENOLS; PLANTS (BOTANY);

AQUEOUS TWO-PHASE SYSTEMS; DOWNSTREAM PROCESSING; ENZYME PURIIFCATION; PROTEIN RECOVERY;

ENZYMES;

CATECHOL OXIDASE; MACROGOL; PHOSPHATE;

AQUEOUS SOLUTION; ARTICLE; ENZYME ACTIVITY; ENZYME PURIFICATION; MOLECULAR WEIGHT; PH; POTATO; PRIORITY JOURNAL; PURIFICATION;

SOLANUM TUBEROSUM;

EID: 31944444062     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2005.10.006     Document Type: Article

References (30)

1. S.J. Brooks, E.M. Doyle, C. Hewage, J.P.G. Malthouse, W. Duetz, and K.E. O' Connor Biotransformation of halophenols using crude cell extracts of Pseudomonas putida F6 Appl. Microbiol. Biotechnol. 64 2004 486 492
2. J. Cabanes, F. Garcia-Canovas, and F. Garcia-Carmona Chemical and enzymatic oxidation of 4-methylcatechol in the presence and absence of l-serine. Spectrophotometric determination of intermediates Biochim. Biophys. Acta 917 1994 190 197
3. J.C. Espin, M. Morales, P.A. Garcia-Ruiz, J. Tudela, and F. Garcia-Canovas Improvement of a continuous spectrophotometric method for determining the monophenolase and diphenolase activities for mushroom polyphenol oxidase J. Agric. Food Chem. 45 1997 1084 1090
4. J.C. Espin, C. Soler-Rivas, E. Cantos, F.A. Tomas-Barberan, and H.J. Wichers Synthesis of the antioxidant hydroxytyrosol using tyrosinase as biocatalyst J. Agric. Food Chem. 49 2001 1187 1193
5. J.N. Rodriquez-Lopez, J. Escribano, and F. Garcia-Canovas A continuous spectrophotometric method for the determination of monophenolase activity of tyrosinase using 3-methyl-2-benzothiazolinone hydrazone Anal. Biochem. 216 1994 205 212
6. P. Pialis, and B.A. Saville Production of l-DOPA from tyrosinase immobilized on nylon 6,6: enzyme stability and scale-up Enzyme Microb. Technol. 22 1998 261 268
7. M. Held, W. Suske, A. Schmid, K.-H. Engesser, H.-P.E. Kohler, and M.G. Wubbolts Preparative scale production of 3-substituted catechols using a novel monooxygenase from Pseudomonas azelaica HBP-1 J. Mol. Catal. B: Enzym. 5 1998 87 93
8. K.L. Kirk, and C.R. Creveling The chemistry and biology of ringfluorinated biogenic amines Med. Res. Rev. 4 1984 189 220
9. A.L. Ghindilis, V.P. Gavrilova, and A.I. Yaropolov Laccase-based biosensor for determination of polyphenols: determination of catechols in tea Biosens. Bioelectron. 7 1992 127 131
10. S.S. Rosatto, L.T. Kubota, and G. Oliveira-Neto Biosensor for phenol based on the direct electron transfer blocking of peroxidase immobilising on silica-titanium Anal. Chim. Acta 390 1999 65 72
11. R.S. Freire, N. Duran, and L.T. Kubota Development of laccase based flow injection electrochemical biosensor for the determination of phenolic compounds and its application for monitering remediation of Kraft E1 paper mill effluent Anal. Chim. Acta 463 2002 229 238
12. K.W. Wissemann, and M.W. Montgomery Purification of d'anjou pear (Pyrus communis L.) polyphenol oxidase Plant Physiol. 78 1985 256
13. C. Marri, A. Frazzoli, A. Hochkoeppler, and V. Poggi Purification of a polyphenol oxidase isoform from potato (Solanum tuberosum) tubers Phytochemistry 63 2003 745 752
14. J.C. Partington, and G.P. Bolwell Purification of polyphenol oxidase free of the storage protein patatin from potato tuber Phytochemistry 42 1996 1499 1502
15. J. Zawistowski, R.J. Weselake, G. Blank, and E.D. Murray Fractionation of Jerusalem artichoke phenolase by immobilized copper affinity chromatography Phytochemistry 26 1987 2905 2907
16. S.P. Kowalski, N.T. Eanetta, A.L. Hirzel, and J.C. Steffens Purification and characterization of polyphenol oxidase from glandular trichomes of Solanum berthaultii Plant Physiol. 100 1992 677
17. S.U. Pathak, and V.S. Ghole Affinity purification and properties of polyphenoloxidase from Solanum tuberosum Phytochemistry 36 5 1994 1165 1167
18. 4 system Biotechnol. Appl. Biochem. 23 1996 237 241
19. E. Andersson, A. Johansson, and G.B. Hagerdal Amylase production in aqueous two-phase system with Bacillus subtilis Enzyme Microb. Technol. 7 1985 333 338
20. S. Tanuja, N.D. Srinivas, K.S.M.S. Raghavarao, and M.K. Gowthaman Aqueous two phase extraction for downstream processing of amyloglucosidase Process Biochem. 32 1997 635 641
21. M. Li, Z.Q. Zhu, and L.H. Mei Partitioning of amino acids by aqueous two-phase systems combined with temperature induced phase formation Biotechnol. Prog. 13 1997 105 108
22. K.S.M.S. Raghavarao, N.K. Rastogi, M.K. Gowthaman, and N.G. Karanth Aqueous two phase extraction for downstream processing of enzymes:proteins Adv. Appl. Microbiol. 41 1995 97 171
23. N.D. Srinivas, K.R. Rashmi, and K.S.M.S. Raghavarao Extraction and purification of a plant peroxidase by aqueous two-phase extraction coupled with gel filtration Process Biochem. 35 1999 43 48
24. O.H. Lowry, N. Roseburgh, A.L. Farr, and R. Randall Protein measurement with the phenol reagent J. Biol. Chem. 193 1951 265 275
25. A.P.B. Rabelo, E.B. Tambourgi, and A. Pessoa Jr. Bromelain partitioning in two-phase aqueous systems containing PEO-PPO-PEO block copolymers J. Chromatogr. B 807 2004 61 68
26. P.A. Alred, A. Kozlowski, J. Milton Harris, and F. Tjerneld Application of temperature-induced phase partitioning at ambient temperature for enzyme purification J. Chromatogr. A 659 1994 289 298
27. M.A. Bim, and T.T. Franco Extraction in aqueous two-phase systems of alkaline xylanase produced by Bacillus pumilus and its application in kraft pulp bleaching J. Chromatogr. B 743 2000 349 356
28. T.T. Franco, A.T. Andrews, and J.A. Asenjo Use of chemically modified proteins to study the effect of a single protein property on partitioning in aqueous two-phase systems: effect of surface hydrophobicity Biotech. Bioeng. 49 1996 300 308
29. F. Tjerneld J.M. Harris Poly(ethylene glycol) Chemistry: Biotechnical and Biomedical Applications 1992 Plenum Press New York 85 102
30. P.A. Albertsson Partition of Cell Particles and Macromolecules third ed. 1986 John Wiley and Sons New York pp. 60-64