Use of microautoradiography to study in situ physiology of bacteria in biofilms

Reviews in Environmental Science and Biotechnology

Volumn 2, Issue 2-4, 2003, Pages 261-268

  Nielsen, Jeppe Lund ^a   Wagner, Michael ^b   Nielsen, Per Halkjœr ^a  

^a AALBORG UNIVERSITY   (Denmark)

^b UNIVERSITY OF VIENNA   (Austria)

Author keywords

Biofilm; Fluorescence in situ hybridization; In situ physiology; Microautoradiography

Indexed keywords

BACTERIA (MICROORGANISMS);

EID: 31944434759     PISSN: 15691705     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:RESB.0000040464.52512.44     Document Type: Review

References (39)

1. Andreasen K & Nielsen PH (1997) Application of microauto-radiography for the study of substrate uptake by filamentous microorganisms in activated sludge. Appl. Environ. Microbiol. 63: 3662-3668
2. Andreasen K & Nielsen PH (2000) Growth of Microthrix parvicella in nutrient removal activated sludge plants: Studies of in situ physiology. Water Res. 34: 1559-1569
3. Bourdier G, Bohatier M & Feuillade J (1989) Amino acid incorporation by natural population of Oscillatoria rubescens. A microautoradiographic study. FEMS Microbiol. Ecol. 62: 185-190
4. Brock TD & Brock ML (1966) Autoradiography as a tool in microbial ecology. Nature 209: 734-736
5. Brock ML & Brock TD (1968) The application of microauto-radiographic techniques to microbial ecology. Fur Theor. Ang. Limnol. 15: 1-29
6. Carman KR (1990) Radioactive labelling of a natural assemblage of marine sedimentary bacteria and microalgae for the trophic studies: An autoradiographic study. Microbial Ecol. 19: 279-290
7. Carman K (1993) Microautoradiographic detection of microbial activity. In: Kemp PF, Sherr BF, Sherr EB & Cole JJ (eds) Handbook of Methods in Aquatic Microbial Ecology (pp 397-404). Lewis Publishers, London
8. Cottrell MT & Kirchman DL (2000) Natural assemblage of marine proteobacteria and members of the Cytophagaflavobacter cluster consuming low and high-molecularweight dissolved organic matter. Appl. Enivron. Microbiol. 66: 1360-1363
9. Daims H, Bruehl A, Amann R, Schleifer KH & Wagner M (1999) The Domain-specific probe EUB338 is insufficient for the detection of all Bacteria: Development and evaluation of a more comprehensive probe set. Syst. Appl. Microbiol. 22: 434-444
10. Daims H, Nielsen JL, Nielsen PH, Schleifer K-H & Wagner M (2001) In situ characterization of Nitrospira-like nitrite-oxidizing bacteria active in wastewater treatment plants. Appl. Environ. Microbiol. 67: 5273-5284
11. Fliermans CB & Schmidt EL (1975) Autoradiography and immuno-fluorescence combined for autecological study of single cell activity with Nitrobacter as a model system. Appl. Microbiol. 30: 676-684
12. Gray ND, Howarth R, Pickup RW, Jones JG & Head IM (2000) Use of combined microautoradiography and fluorescence in situ hybridization to determine carbon metabolism in mixed natural communities of uncultured bacteria from the genus Achromatium. Appl. Environ. Microbiol. 66: 4518-4522
13. Grossmann S (1994) Bacterial activity in sea ice and open water of the Weddel Sea, Antarctica: A microautoradiographic study. Microbial. Ecol. 28: 1-18
14. Hermansson M & Dahlback B (1983) Bacterial activity at the air/water interface. Microbial Ecol. 9: 317-328
15. Hoppe H-G (1976) Determination and properties of actively metabolizing heterotrophic bacteria in the sea, investigated by means of micro-autoradiography. Mar. Bio. 36: 291-302
16. Ito T, Nielsen JL, Okabe S, Watanabe Y & Nielsen PH (2002) Phylogenetic identification and substrate uptake patterns of sulfate-reducing bacteria inhabiting an aerobic-anaerobic sewer biofilm. Appl. Environ. Microbiol. 68: 356-364
17. Lee N, Nielsen PH, Andreasen K, Juretschko S, Nielsen JL, Schleifer K-H & Wagner M (1999) Combination of fluorescent in situ hybridization and microautoradiography-a new tool for structure-function analysis in microbial ecology. Appl. Environ. Microbiol. 65: 1289-1297
18. Lee N, Nielsen PH, Aspegren H, Henze M, Schleifer K-H & la Cour Jansen J (2003) Long-term population dynamics and in situ physiology in activated sludge systems with enhanced biological phosphorus removal operated with and without nitrogen removal. Sys. Appl. Microbiol. 26: 211-227
19. Meyer-Reil L-A (1978) Autoradiography and epifluorescence microscopy combined for the determination of number and spectrum of actively metabolizing bacteria in natural waters. Appl. Environ. Microbiol. 36: 506-512
20. Nielsen PH, Andreasen K, Lee N & Wagner M (1999) Use of microautoradiography and fluorescent in situ hybridization for characterization of microbial activity in activated sludge. Water Sci. Technol. 39(1): 1-9
21. Nielsen PH, Aquino de Muro M & Nielsen JL (2000) Studies on the in situ physiology of Thiothrix spp. in activated sludge. Environ. Microbiol. 2: 389-398
22. Nielsen JL, Christensen D, Kloppenborg M & Nielsen PH (2003) Quantification of cell-specific substrate uptake by probe-defined bacteria under in situ conditions by microautoradiography and fluorescence in situ hybridization. Environ. Microbiol. 5(3): 202-211
23. Nielsen JL & Nielsen PH (2002a) Enumeration of acetate-consuming bacteria by microautoradiography under oxygen-and nitrate respiring conditions in activated sludge. Water Res. 36: 421-428
24. Nielsen JL & Nielsen PH (2002b) Enumeration of functional bacterial groups in activated sludge by microautoradiography. Water Sci. Tech. 46(1-2): 389-395
25. Nielsen PH, Roslev P, Dueholm T & Nielsen JL (2002) Microthrix parvicella, a specialized lipid consumer in anaerobic-aerobic activated sludge plants Water Sci. Technol. 46(1-2): 73-80
26. Nilsson C & Sundback K (1996) Amino acid uptake in natural microphytobenthic assemblages studied by microautoradiography. Hydrobiologia 332: 119-129
27. Ouverney CC & Fuhrman JA (1999) Combined microautoradiography-16S rRNA probe technique for determination of radioisotope uptake by specific microbial cell type in situ. Appl. Enivron. Microbiol. 65: 1746-1752
28. Ouverney CC & Fuhrman JA (2000) Marine planktonic Archaea take up amino acids. Appl. Enivron. Microbiol. 66: 4829-4833
29. Pearl HW (1984) Alteration of metabolic activities in association with detritus. Bull. Mar. Sci. 35: 393-408
30. Pearl HW (1991) Ecophysiological and tropical implications of light-stimulated amino acid utilization in marine picoplankton. Appl. Environ. Microbiol. 57: 473-479
31. Pearl HW & Priscu JC (1998) Microbial phototrophic, heterotrophic and diazotrophic activities associated with aggregates in the permanent ice cover of lake Bonney, Antarctica. Microbial Ecol. 36: 221-230
32. Pearl HW & Stull EA (1979) In defense of grain density autoradiography. Limnol. Oceanogr. 24: 1166-1169
33. Pedros-Alio C & Nedwell S (1989) Microautoradiographic study of thymidine uptake in brackish waters around Sapelo Island, Georgia, USA. Mar. Ecol-Prog. Ser. 55: 83-94
34. Rogers AW (1979) Techniques of Autoradiography. Elsevier, New York
35. Roszak DB & Colwell RR (1987) Metabolic activity of bacteria cells enumerated by direct viable count. Appl. Environ. Microbiol. 53: 2889-2983
36. Tabor SP & Neihof RA (1982) Improved microautoradiographic method to determine individual microorganisms active in substrate uptake in natural waters. Appl. Environ. Microbiol. 44: 945-953
37. Tabor SP & Neihof RA (1984) Direct determination of activities for microorganisms of Chesapeake Bay populations. Appl. Environ. Microbiol. 48: 1012-1019
38. Thomsen TR, Kjellerup BV, Nielsen JL, Hugenholtz P & Nielsen PH (2002) In situ studies of the phylogeny and physiology of filamentous bacteria with attached growth. Environ. Microbiol. 4: 383-391
39. Vaniman DA, Furlano S, Chipera J, Thompson K & Triay I (1996) Microautoradiography in studies of Pu(V) sorption by trace and fracture minerals in tuff. Mat. Res. Soc. Symp. Proc. 412: 639-646