Phylogenetic identification and substrate uptake patterns of sulfate-reducing bacteria inhabiting an oxic-anoxic sewer biofilm determined by combining microautoradiography and fluorescent in situ hybridization

Applied and Environmental Microbiology

Volumn 68, Issue 1, 2002, Pages 356-364

  Ito, T ^a   Nielsen, J L ^a   Okabe, S ^a   Watanabe, Y ^a   Nielsen, P H ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOFILMS; CELLS; DNA; FLUORESCENCE; NITRATES; RADIOGRAPHY; RNA;

PHYLOGENETIC IDENTIFICATION;

BACTERIA;

CARBON 14; CARBON DIOXIDE; HYDROGEN; NITRATE; OXYGEN; PROPIONIC ACID; RIBOSOME DNA; RNA 16S; SULFATE;

PHYLOGENETICS;

AEROBIC METABOLISM; ANAEROBIC METABOLISM; ARTICLE; AUTORADIOGRAPHY; BACTERIAL METABOLISM; BACTERIUM IDENTIFICATION; BIODIVERSITY; BIOFILM; CARBON DIOXIDE FIXATION; CONTROLLED STUDY; DESULFOBULBUS; DESULFOMICROBIUM; DESULFOVIBRIO; ELECTRON TRANSPORT; FATTY ACID TRANSPORT; FLUORESCENCE IN SITU HYBRIDIZATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; RNA PROBE; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SEWER; SPECIES DIFFERENCE; SULFATE REDUCING BACTERIUM;

AEROBIOSIS; ANAEROBIOSIS; AUTORADIOGRAPHY; BIOFILMS; DELTAPROTEOBACTERIA; DNA, RIBOSOMAL; IN SITU HYBRIDIZATION, FLUORESCENCE; MOLECULAR SEQUENCE DATA; PHYLOGENY; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SEWAGE; SULFATES; SULFUR-REDUCING BACTERIA;

BACTERIA (MICROORGANISMS); DESULFOBULBUS; DESULFOMICROBIUM; DESULFOMICROBIUM HYPOGEIUM; DESULFOVIBRIO; DESULFOVIBRIO DESULFURICANS;

EID: 0036136673     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.1.356-364.2002     Document Type: Article

References (39)

1. Basic local alignment search tool
2. In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes
3. Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms
4. Application of microautoradiography to the study of substrate uptake by filamentous microorganisms in activated sludge
5. Autoradiography as a tool in microbial ecology
6. Spectrophotometric determination of hydrogen sulfide in natural waters
7. Development of oligonucleotide probes and PCR primers for detecting phylogenetic subgroups of sulfate-reducing bacteria
8. 2 or nitrate by sulfate-reducing bacteria
9. Genus- and group-specific hybridization probes for determinative and environmental studies of sulfate-reducing bacteria
10. Physiology, phylogenetic relationships, and ecology of filamentous sulfate-reducing bacteria (genus Desulfonema)
11. Use of combined microautoradiography and fluorescence in situ hybridization to determine carbon metabolism in mixed natural communities of uncultured bacteria from the genus Achromatium
12. Use of nucleopore filters for counting bacteria by fluorescence microscopy
13. The sulfur cycle of a coastal marine sediment (Limfjorden, Denmark)
14. Mineralization of organic matter in the sea bed-the role of sulfate reduction
15. A sulfate-reducing bacterium from the oxic layer of a microbial mat from Solar Lake (Sinai), Desulfovibrio oxyclinae sp. nov.
16. 2-utilizing bacteria, Desulfomicrobium hypogeium sp. nov. and Acetobacterium psammolithicum sp. nov., and their ecological roles
17. Microsensor measurement of sulfate reduction and sulfide oxidation in compact microbial communities of aerobic biofilms
18. Combination of fluorescent in situ hybridization and microautoradiography - A new tool for structure-function analyses in microbial ecology
19. The RDP (Ribosomal Database Project)
20. Abundance and spatial organization of gram-negative sulfate-reducing bacteria in activated sludge investigated by in situ probing with specific 16S rRNA targeted oligonucleotides
21. Unexpected population distribution in a microbial mat community: Sulfate-reducing bacteria localized to the highly oxic chemocline in contrast to a eukaryotic preference for anoxia
22. Diversity of sulfate-reducing bacteria in oxic and anoxic regions of a microbial mat characterized by comparative analysis of dissimilatory sulfite reductase genes
23. Studies on the in situ physiology of Thiothrix spp. in activated sludge
24. Analyses of spatial distribution of sulfate-reducing bacteria and their activity in aerobic wastewater biofilms
25. Combined microautoradiography-16S rRNA probe technique for determination of radioisotope uptake by specific microbial cell types in situ
26. Degradative capacities and 16S rRNA-targeted whole cell hybridization of sulfate-reducing bacteria in an anaerobic environment culture utilizing alkylbenzenes from crude oil
27. Distribution of bacterial populations in a stratified fjord (Mariager Fjord, Denmark) quantified by in situ hybridization and related to chemical gradients in the water column
28. 2S in photosynthetic biofilms determined by oligonucleotide probe and microelectrodes
29. Community structure of a microbial mat: The phylogenetic dimension
30. Phylogenetic and physiological diversity of sulphate-reducing bacteria isolated from a salt marsh sediment
31. The neighbor-joining method: A new method for constructing phylogenetic trees
32. Structural and functional dynamics of sulfate-reducing populations in bacterial biofilms
33. Vertical distribution of sulfate-reducing bacteria at the oxic-anoxic interface in sediments of the oligotrophic Lake Stechlin
34. Distribution of sulfate-reducing bacteria in a stratified fjord (Mariager Fjord, Denmark) as evaluated by most-probable-number counts and denaturing gradient gel electrophoresis of PCR-amplified ribosomal DNA fragments
35. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
36. Phylogeny of dissimilatory sulfite reductases supports an early origin of sulfate respiration
37. Microbiology and ecology of sulfate-and sulfur-reducing bacteria
38. Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. II. Incomplete oxidation of propionate by Desulfobulbus propionicus gen. nov., sp. nov.