Reduced toxicity of lipo-oligosaccharide from a phoP mutant of Neisseria meningitidis: An in vitro demostration

Journal of Endotoxin Research

Volumn 12, Issue 1, 2006, Pages 39-46

  Rustam, Tarick ^a   McClean, Stephen ^b   Newcombe, Jane ^c   McFadden, Johnjoe ^c   Eales Reynolds, Lesley Jane ^a  

^a UNIVERSITY OF PORTSMOUTH   (United Kingdom)

^b UNIVERSITY OF ULSTER   (United Kingdom)

^c UNIVERSITY OF SURREY   (United Kingdom)

Author keywords

Lipo oligosaccharide; Neisseria meningitidis; PhoP; Pro inflammatory mediators; TNF alpha; Two component regulatory system

Indexed keywords

CYTOKINE; LIPOOLIGOSACCHARIDE; MUTANT PROTEIN; NITROGEN; PHOP PROTEIN; REACTIVE OXYGEN METABOLITE;

ARTICLE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; CONTROLLED STUDY; CYTOKINE RELEASE; HUMAN; HUMAN CELL; IMMUNOTOXICITY; IN VITRO STUDY; INFLAMMATION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR MODEL; NEISSERIA MENINGITIDIS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; REGULATORY MECHANISM; STRAIN DIFFERENCE; WILD TYPE;

BACTERIAL PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FREE RADICALS; HUMANS; LIPOPOLYSACCHARIDES; MONOCYTES; MUTATION; NEISSERIA MENINGITIDIS; NITROGEN; PLASMIDS; REACTIVE OXYGEN SPECIES; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TUMOR NECROSIS FACTOR-ALPHA;

BACTERIA (MICROORGANISMS); NEISSERIA MENINGITIDIS;

EID: 31644436459     PISSN: 09680519     EISSN: None     Source Type: Journal    
DOI: 10.1179/096805106X89116     Document Type: Article

References (28)

1. Apicella MA. Neisseria meningitides. In: Mandell GL, Bennett JE, Dolin R. (eds) Principles and Practice of Infectious Diseases, vol 2,5th edn. Philadelphia, PA: Churchill Livingstone, 2000; 2228-2241.
2. Rosenstein NE, Perkins BA, Stephens DS, Popovic T, Hughes JM. Meningococcal disease. N Engl J Med 2001; 344: 1378-1388.
3. Galanos C. Physical state and biological activity of lipopolysaccharides. Toxicity and inummogenicity of the lipid A component. Z Immunol Forsch 1975; 149: 214-219.
4. Kutshin VA, Zabringer U, Lindner B et al. Structural characterization of the lipid A component of pathogenic Neisseria meningitidis. J Bacteriol 1992; 174: 1793-1800.
5. Raetz CRH. Bacterial endotoxins: extraordinary lipids that activate eukaryotic signal transduction. J Bacteriol 1993; 175: 5745-5753.
6. Lee N, Sunshine M, Engstrom J, Gibson B, Apicella MA. Mutation of the htrB locus of Haemophilus influenzae non-typeable strain 2019 is associated with modifications of lipid A and phosphorylation of the lipooligosacchatide. J Biol Chem 1995;270:27151-27159.
7. Sunshine M, Gibson BW, Engstrom JJ, Nichols WA, Jones BD, Apicella MA. Mutation of the htrB gene in a virulent Salmonella typhimurium strain by intergenic transduction; strain construction and phenotypic characterization. J Bacteriol 1997; 179: 5521-5533.
8. Kahler CM, Stephens DS. Genetic bases for biosynthesis, structure and function of meningococcal lipo-oligosaccharide. Crit Rev Microbiol 1998; 24: 281-334.
9. Rahman MM, Stephens DS, Kahler CM, Glushka J, Carlson RW. The lipooligosaccharide (LOS) of Neisseria meningitidis serogroup B strain NMB contains L2, L3, and novel oligosaccharides, and lacks the lipid-A 4′-phosphate substituent. Carbohydr Res 1998; 307: 311-324.
10. Brandenburg Y, Wiese A. Endotoxins: relationships between structure, function and activity. Curr Top Med Chem 2004; 4. 112-146.
11. Groisman EA, Chiao E, Lipps CJ, Heffron R Salmonella typhimurium phoP virulence gene is a transcriptional regulator. Proc Natl Acad Sci USA 1989; 86: 7077-7081.
12. Groisman EA. The pleiotropic two-component regulatory system PhoP-PhoQ. J Bacteriol 2001; 183: 1835-1842.
13. Hitchen PG, Prior JL, Oyston PC et al. Structural characterization of lipo-oligosaccharide (LOS) from Yersinia pestis: regulation of LOS structure by the PhoPQ system. Mol Microbiol 2002; 44: 1637-1650.
14. Guo L, Lim KB, Poduje CM et al. Lipid A acylation and bacterial resistance against vertebrate antimicrobial peptides. Cell 1998;95: 189-198.
15. Trent MS, Pabich W, Raetz CR, Miller SI. A PhoP/PhoQ-induced lipase (PagL) that catalyzes 3-O-deacylation of lipid A precursors in membranes of Salmonella typhimurium. J Biol Chem 2001; 276: 9083-9092.
16. Guo L, Lim KB, Gunn JS et al. Regulation of lipid A modifications by Salmonella typhimurium virulence genes phoP-phoQ. Science 1997; 276 250-253.
17. Johnson CR, Newcombe J, Thorne S et al. Generation and characterisation of a PhoP homologue mutant of Neisseria meningitidis. Mol Microbiol 2001; 39: 1345-1355.
18. Newcombe J, Eales-Reynolds LJ, Wootton L et al. Infection with an avirulent phoP mutant of Neisseria meningitidis confers broad cross-reactive immunity. Infect Immun 2004; 72: 338-344.
19. Steeghs L, Tommassen J, Leusen JH, van de Winkel JG, van der Ley P, Teasing apart structural determinants of 'toxicity' and 'adjuvanticity': implications for meningococcal vaccine development. J Endoxin Res 2004; 10: 113-119.
20. Braun JM, Backwell CC, Poxton IR et al. Proinflammatory responses to lipo-oligosaccharide of Neisseria meningitides immunotype strains in relation to virulence and disease. J Infect Dis 2002; 185: 1431-1438.
21. Zughaier SM, Tzeng YL, Zimmer SM, Datta A, Carlson RW, Stephens DS. Neisseria meningitides lipooligosaccharide structure-dependent activation of the macrophage CD14/Toll-like receptor 4 pathway. Infect Immun 2004; 72: 371-380.
22. Uronen-Hansson H, Steeghs L, Allen J et al. Human dendritic cell activation by Neisseria meningitidis: phagocytosis depends on expression of lipooligosaccharide (LOS) by the bacteria and is required for optimal cytokine production. Cell Microbiol 2004; 6: 625-637.
23. Westphal O, Jann K. Bacteria] lipopolysaccharides: extraction with phenol-water and further applications of the procedure. In: Whistler RL. (ed) Methods in Carbohydrate Chemistry. New York, NY. Academic Press, 1965; 83-101.
24. Saha DC, Barua RS, Astiz MF, Rackow EC, Eales-Reynolds LJ. Monophosphoryl lipid A stimulated up-regulation of reactive oxygen intermediates in human monocytes in vitro. J Leukoc Biol 2001;70:381-385.
25. Seydel U, Brandenburg K, Rietschel ET. A case for an endotoxic conformation. Prog Clin Biol Res 1994; 388: 17-30.
26. Alexander C, Rietschel ET. Bacterial lipopolysaccharides and innate immunity. J Endotoxin Res 2001; 7: 167-202.
27. Gibson BW, Melaugh W, Phillips NJ, Apicella MA, Campagnari AA, McLeod Griffiss J. Investigation of the structural heterogeneity of lipooligosaccharides from pathogenic Haernophilus and Neisseria species and of R-type lipopolysaccharides from Salmonella typhimurnmi by electrospray mass spectrometry. J Bacteriol 1993; 175: 2702-2712.
28. Tzeng YL, Datta A, Ambrose K et al. The MisR/MisS two-component regulatory system influences inner core structure and immunotype of lipooligosaccharide in Neisseria meningitidis. J Biol Chem 2004; 279; 35053-35062.