Identification and characterization of a Na+-dependent neutral amino acid transporter, ASCT1, in rabbit corneal epithelial cell culture and rabbit cornea

Current Eye Research

Volumn 30, Issue 11, 2005, Pages 989-1002

  Katragadda, Suresh ^a   Talluri, Ravi Sankar ^a   Pal, Dhananjay ^a   Mitra, Ashim K ^a  

^a UNIVERSITY OF MISSOURI KANSAS CITY   (United States)

Author keywords

Antiviral drugs; Corneal epithelium; Drug delivery; Membrane transport; Transporters

Indexed keywords

ALANINE; AMINO ACID TRANSPORTER; CYSTEINE; OUABAIN; SERINE; SODIUM AZIDE; THREONINE;

AMINO ACID SEQUENCE; AMINO ACID TRANSPORT; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CONTROLLED STUDY; CORNEA; CORNEA EPITHELIUM; ENERGY; EPITHELIUM CELL; MALE; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SODIUM TRANSPORT;

ALANINE; AMINO ACID TRANSPORT SYSTEMS, NEUTRAL; ANIMALS; BINDING, COMPETITIVE; BIOLOGICAL TRANSPORT; CELLS, CULTURED; EPITHELIUM, CORNEAL; HYDROGEN-ION CONCENTRATION; IONS; KINETICS; MALE; MANNITOL; OSMOLAR CONCENTRATION; RABBITS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SODIUM;

EID: 31544435477     PISSN: 02713683     EISSN: None     Source Type: Journal    
DOI: 10.1080/02713680500306439     Document Type: Article

References (45)

1. Dey S, Anand BS, Patel J, Mitra AK. Transporters/receptors in the anterior chamber: pathways to explore ocular drug delivery strategies. Expert Opin Biol Ther. 2003;3:23-44.
2. Anand BS, Mitra AK. Mechanism of corneal permeation of L-valyl ester of acyclovir: targeting the oligopeptide transporter on the rabbit cornea. Pharm Res 2002;19:1194-1202.
3. Jain-Vakkalagadda Blisse PD, Gunda Sriram, Nashed Yasser,Ganapathy Vadivel, Ashim K. Mitra. Identification of a Na+-dependent cationic and neutral amino acid transporter, B0,+, in human and rabbit cornea. Mol. Pharm. 2004;1:338-346.
4. B. Jain-Vakkalagadda, Dey S, Pal D, Mitra AK. Identification and functional characterization of a Na+-independent large neutral amino acid transporter, LAT1, in human and rabbit cornea. Invest Ophthalmol Vis Sci. 2003;44:2919-2927.
5. Takahashi H, Ohara K, Ohmura T, Takahashi R, Zieske JD. Glucose transporter 1 expression in corneal wound repair under high serum glucose level. Jpn J Ophthalmol. 2000;44:470-474.
6. Takahashi H, Kaminski AE, Zieske JD. Glucose transporter 1 expression is enhanced during corneal epithelial wound repair. Exp Eye Res. 1996;63:649-659.
7. Bonanno JA. Lactate-proton cotransport in rabbit corneal epithelium. Curr Eye Res. 1990;9:707-712.
8. ChenZhuo L, Murube J, Latorre A, R. M. del Rio. Different concentrations of amino acids in tears of normal and human dry eyes. Adv Exp Med Biol. 2002;506:617-621.
9. Christensen HN. Interorgan amino acid nutrition. Physiol Rev. 1982;62:1193-1233.
10. Pardridge WM. Molecular biology of the blood-brain barrier. Methods Mol Med. 2003;89:385-399.
11. Ganapathy V BM, Leibach FH. Physiology of the gastrointestinal tract. In Johnson LR. Intestinal transport of amino acids and peptides. New York: Raven Press; 1994:1773-1794.
12. Kilberg MS, Handlogten ME, Christensen HN. Characteristics of system ASC for transport of neutral amino acids in the isolated rat hepatocyte. J Biol Chem. 1981;256:3304-3312.
13. Goldenberg GJ, Lam HY, Begleiter A. Active carrier-mediated transport of melphalan by two separate amino acid transport systems in LPC-1 plasmacytoma cells in vitro. J Biol Chem. 1979;254:1057-1064.
14. Blondeau JP, Beslin A, Chantoux F, Francon J. Triiodothyronine is a high-affinity inhibitor of amino acid transport system L1 in cultured astrocytes. J Neurochem. 1993;60:1407-1413.
15. Lakshmanan M, Goncalves E, Lessly G, Foti D, Robbins J. The transport of thyroxine into mouse neuroblastoma cells, NB41A3: the effect of L-system amino acids. Endocrinology. 1990;126:3245-3250.
16. Su TZ, Lunney E, Campbell G, Oxender DL. Transport of gabapentin, a gamma-amino acid drug, by system I alpha-amino acid transporters: a comparative study in astrocytes, synaptosomes, and CHO cells. J Neurochem. 1995;64:2125-2131.
17. Hatanaka T, Haramura M, Fei YJ, Miyauchi S, Bridges CC, Ganapathy PS, Smith SB, Ganapathy V, Ganapathy ME. Transport of amino acid-based prodrugs by the Na+- and Cl(-) -coupled amino acid transporter ATBO,+ and expression of the transporter in tissues amenable for drug delivery. J Pharmacol Exp Ther. 2004;308:1138-1147.
18. Christensen HN. Role of amino acid transport and countertransport in nutrition and metabolism. Physiol Rev. 1990;70:43-77.
19. Arriza JL, Kavanaugh MP, Fairman WA, Wu YN, Murdoch GH, North RA, Amara SG. Cloning and expression of a human neutral amino acid transporter with structural similarity to the glutamate transporter gene family. J Biol Chem. 1993;268:15329-15332.
20. Shafqat S, Tamarappoo BK, Kilberg MS, Puranam RS, McNamara JO, A. Guadano-Ferraz, Fremeau RT, Jr. Cloning and expression of a novel Na(+)-dependent neutral amino acid transporter structurally related to mammalian Na+/glutamate cotransporters. J Biol Chem. 1993;268:15351-15355.
21. Zerangue N and Kavanaugh MP. ASCT-1 is a neutral amino acid exchanger with chloride channel activity. J Biol Chem. 1996;271:27991-27994.
22. Christensen HN, Liang M, Archer EG. A distinct Na+-requiring transport system for alanine, serine, cysteine, and similar amino acids. J Biol Chem. 1967;242:5237-5246.
23. Howard A, Goodlad RA, Walters JR, Ford D, Hirst BH. Increased expression of specific intestinal amino acid and peptide transporter mRNA in rats fed by TPN is reversed by GLP-2. J Nutr 2004;134:2957-2964.
24. Yamamoto T, Nishizaki I, Nukada T, Kamegaya E, Furuya S, Hirabayashi Y, Ikeda K, Hata H, Kobayashi H, Sora I, Yamamoto H. Functional identification of ASCT1 neutral amino acid transporter as the predominant system for the uptake of L-serine in rat neurons in primary culture. Neurosci Res. 2004;49:101-111.
25. Yamamoto T, Nishizaki I, Furuya S, Hirabayashi Y, Takahashi K, Okuyama S, Yamamoto H. Characterization of rapid and high-affinity uptake of L-serine in neurons and astrocytes in primary culture. FEBS Lett. 2003;548:69-73.
26. Furuyaand S, Watanabe M. Novel neuroglial and glioglial relationships mediated by L-serine metabolism. Arch Histol Cytol. 2003;66:109-121.
27. Younes M, Pathak M, Finnie D, Sifers RN, Liu Y, Schwartz MR. Expression of the neutral amino acids transporter ASCT1 in esophageal carcinomas. Anticancer Res. 2000;20:3775-3779.
28. Liaoand K, Lane MD. Expression of a novel insulin-activated amino acid transporter gene during differentiation of 3T3-L1 preadipocytes into adipocytes. Biochem Biophys Res Commun. 1995;208:1008-1015.
29. Utsunomiya-Tate N, Endou H, Kanai Y. Cloning and functional characterization of a system ASC-like Na+-dependent neutral amino acid transporter. J Biol Chem. 1996;271:14883-14890.
30. Tetsuka K, Takanaga H, Ohtsuki S, Hosoya K, Terasaki T. The I-isomer-selective transport of aspartic acid is mediated by ASCT2 at the blood-brain barrier. J Neurochem. 2003;87:891-901.
31. Fuchs BC, Perez JC, Suetterlin JE, Chaudhry SB, Bode BP. Inducible antisense RNA targeting amino acid transporter ATB0/ASCT2 elicits apoptosis in human hepatoma cells. Am J Physiol Gastrointest Liver Physiol. 2004;286:G467-G478.
32. Kanai Y, Smith CP, Hediger MA. A new family of neurotransmitter transporters: the high-affinity glutamate transporters. FASEB J. 1993;7:1450-1459.
33. Pinilla-Tenas J, Barber A, Lostao MP. Transport of proline and hydroxyproline by the neutral amino-acid exchanger ASCT1. J Membr Biol. 2003;195:27-32.
34. Kekuda R, Torres-Zamorano V, Fei YJ, Prasad PD, Li HW, Mader LD, F. Leibach, H, Ganapathy V. Molecular and functional characterization of intestinal Na(+)-dependent neutral amino acid transporter B0. Am J Physiol. 1997;272:G1463-472.
35. Dey S, Patel J, Anand BS, Jain-Vakkalagadda B, Kaliki P, Pal D, Ganapathy V, Mitra AK. Molecular evidence and functional expression of P-glycoprotein (MDR1) in human and rabbit cornea and corneal epithelial cell lines. Invest Ophthalmol Vis Sci. 2003;44:2909-2918.
36. Tak RV, Pal D, Gao H, Dey S, Mitra AK. Transport of acyclovir ester prodrugs through rabbit cornea and SIRC-rabbit corneal epithelial cell line. J Pharm Sci. 2001;90:1505-1515.
37. Sugawara M, Nakanishi T, Fei YJ, Huang W, Ganapathy ME, Leibach FH, Ganapathy V. Cloning of an amino acid transporter with functional characteristics and tissue expression pattern identical to that of system A. J Biol Chem 2000;275:16473-16477.
38. Peter GJ, Davies A, Watt PW, Birrell J, Taylor PM. Interactions between the thiol-group reagent N-ethylmaleimide and neutral and basic amino acid transporter-related amino acid transport. Biochem J. 1999;343 Pt 1:169-176.
39. Laspiur JP, Burton JL, Weber PS, Kirkwood RN, Trottier NL. Short communication: amino acid transporters in porcine mammary gland during lactation. J Dairy Sci. 2004;87:3235-3237.
40. Irie M, Terada T, Sawada K, Saito H, Inui K. Recognition and transport characteristics of nonpeptidic compounds by basolateral peptide transporter in Caco-2 cells. J Pharmacol. Exp. Ther. 2001;298:711-717.
41. Pinilla J, Barber A, Lostao MP. Active transport of alanine by the neutral amino-acid exchanger ASCT1. Can J Physiol Pharmacol. 2001;79:1023-1029.
42. Makowskeand M, Christensen HN. Contrasts in transport systems for anionic amino acids in hepatocytes and a hepatoma cell line HTC. J Biol Chem. 1982;257:5663-5670.
43. Vadgamaand JV, Christensen HN. Wide distribution of pH-dependent service of transport system ASC for both anionic and zwitterionic amino acids. J Biol Chem. 1984;259:3648-3652.
44. Castagna M, Shayakul C, Trotti D, Sacchi VF, Harvey WR, Hediger MA. Molecular characteristics of mammalian and insect amino acid transporters: implications for amino acid homeostasis. J Exp Biol. 1997;200(Pt 2):269-286.
45. Winterand CG, Christensen HN. Contrasts in neutral amino acid transport by rabbit erythrocytes and reticulocytes. J Biol Chem. 1965;240:3594-3600.