Alteration of leucine aminopeptidase from Streptomyces septatus TH-2 to phenylalanine aminopeptidase by site-directed mutagenesis

Applied and Environmental Microbiology

Volumn 71, Issue 11, 2005, Pages 7229-7235

  Arima, Jiro ^a   Uesugi, Yoshiko ^a   Iwabuchi, Masaki ^a   Hatanaka, Tadashi ^a  

^a Research Institute for Biological Science OKAYAMA   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCATALYSTS; HYDROLYSIS; MUTAGENESIS; POLYPEPTIDES; REACTION KINETICS; SATURATION (MATERIALS COMPOSITION);

HYDROLYTIC ACTIVITY; LEUCINE AMINOPEPTIDASE; N-TERMINAL RESIDUE; STREPTOMYCES SEPTATUS TH-2 (SSAP);

ENZYME KINETICS;

4 NITROANILINE; CYTOSOL AMINOPEPTIDASE; LEUCINE; PHENYLALANINE AMINOPEPTIDASE; PHENYLALANINE DERIVATIVE; UNCLASSIFIED DRUG;

ENZYME;

AMINO TERMINAL SEQUENCE; ARTICLE; BIOCATALYST; ENZYME SPECIFICITY; HYDROLYSIS; KINETICS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; SITE DIRECTED MUTAGENESIS; STREPTOMYCES; STREPTOMYCES SEPTATUS; THERMOSTABILITY;

AMINOPEPTIDASES; KINETICS; LEUCYL AMINOPEPTIDASE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PHENYLALANINE; STREPTOMYCES; SUBSTRATE SPECIFICITY;

STREPTOMYCES; STREPTOMYCES SEPTATUS;

EID: 31444450427     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.11.7229-7235.2005     Document Type: Article

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