Optimisation of a serine protease coupling to Eudragit S-100 by experiment design techniques

Journal of Chemical Technology and Biotechnology

Volumn 81, Issue 1, 2006, Pages 8-16

  Silva, Carla J S M ^a   Gübitz, Georg ^b   Cavaco Paulo, Artur ^a  

^a UNIVERSITY OF MINHO   (Portugal)

^b GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

Coupling; Eudragit; Factorial designs; Serine proteases

Indexed keywords

AGENTS; CHEMICAL REACTIONS; CONCENTRATION (PROCESS); ELECTROPHORESIS; ENZYME IMMOBILIZATION; GELS; OPTIMIZATION; POLYMERS;

BLOCKING AGENT CONCENTRATION; CARBODIIMIDE CONCENTRATION; EUDRAGIT; FACTORIAL DESIGN; POLYMER CONCENTRATION; SERINE PROTEASE COUPLING; SERINE PROTEASES; TIME OF REACTION;

ENZYMES;

CYANAMIDE; EUDRAGIT; POLYMER; SERINE PROTEINASE;

AGENTS; CHEMICAL REACTIONS; CONCENTRATION (PROCESS); ELECTROPHORESIS; ENZYME IMMOBILIZATION; ENZYMES; GELS; OPTIMIZATION; POLYMERS;

ARTICLE; CHEMICAL REACTION; CONCENTRATION RESPONSE; CONTROLLED STUDY; CROSS COUPLING REACTION; CROSS LINKING; ENZYME ASSAY; POLYACRYLAMIDE GEL ELECTROPHORESIS; REACTION OPTIMIZATION; REACTION TIME; TEMPERATURE SENSITIVITY;

EID: 31444440561     PISSN: 02682575     EISSN: 10974660     Source Type: Journal    
DOI: 10.1002/jctb.1350     Document Type: Article

References (20)

1. Heine E and Höcker H, Enzyme treatments for wool and cotton. Rev Prog Coloration 25:57-63 (1995).
2. Fujimura M, Mori T and Tosa T, Preparation and properties of soluble-insoluble immobilized proteases. Biotechnology and Bioengineering 29:747-752 (1987).
3. Taniguchi M, Kobayashi M and Fujii M, Properties of a reversible soluble-insoluble cellulose and its application to repeated hydrolysis of crystalline cellulose. Biotechnology and Bioengineering 34:1092-1097 (1989).
4. Silva CJSM, Sousa F, Gübitz G and Cavaco-Paulo A, Chemical modifications on proteins using glutaraldehyde. Food Technol Biotechnol 42:51-56 (2004).
5. Rodrigues AR, Cabral JMS and Taipa MA, Immobilization of Chromobacterium viscosum lipase on Eudragit S-100: coupling, characterization and kinetic application in organic and biphasic media. Enzyme and Microbial Technology 31:133-141 (2002).
6. Sardar M, Agarwal R, Kumar A and Gupta MN, Noncovalent immobilization of enzymes on an enteric polymer Eudragit S-100. Enzyme and Microbial Technology 20:361-367 (1997).
7. Tyagi R, Roy I, Agarwal R and Gupta MN, Carbodiimide coupling of enzymes to the reversibly soluble insoluble polymer Eudragit S-100. Biotechnol Appl Biochem 28:201-206 (1998).
8. Arasaratnam V, Galaev IY and Mattiasson B, Reversibly soluble biocatalyst: optimization of trypsin coupling to Eudragit S-100 and biocatalyst activity in soluble and precipitated forms. Enzyme and Microbial Technology 27:254-263 (2000).
9. Silva CJSM and Roberto IC, Statistical screening method for selection of important variables on xylitol biosynthesis from rice straw hydrolysate by Candida guilliermondii FTI 20 037. Biotechnology Techniques 13:743-747 (1999).
10. Serralha FN, Lopes JM, Lemos F, Ramôa Ribeiro F, Prazeres DMF, Aires-Barros MR and Cabral JMS, Application of factorial design to the study of an alcoholysis transformation promoted by cutinase immobilized on NaY zeolite and Accurel PA6. Journal of Molecular Catalysis B: Enzymatic 27:19-27 (2004).
11. Moyo S, Gashe BA, Collison EK and Mpuchane S, Optimising growth conditions for the pectinolytic activity of Kluyveromyces wickerhamii by using response surface methodology. International Journal of Food Microbiology 85:87-100 (2003).
12. Cortez EV, Pessoa Jr A, Felipe MGA, Roberto IC and Vitolo M, Optimized extraction by cetyl trimethyl ammonium bromide reversed micelles of xylose reductase and xylitol dehydrogenase from Candida guilliermondii homogenate. Journal of Chromatography B 807:47-54 (2004).
13. Mayerhoff ZDVL, Roberto IC and Franco TT, Purification of xylose reductase from Candida mogii in aqueous two-phase systems. Biochemical Engineering Journal 18:217-223 (2004).
14. Box GEP, Hunter WG and Hunter SJ, Statistics for Experimenters. Wiley, New York (1978).
15. Lowry OH, Rosebrough NJ, Farr AL and Randall RJ, Quantitation of protein using Folin Ciocalteau reagent. J Biol Chem 193:265-275 (1951).
16. Laemmli UK, Cleavage of structural proteins during assembly of head of bacteriofage-T4. Nature 227:680-685 (1970).
17. Dourado F, Bastos M, Mota M and Gama FM, Studies on the properties of Celluclast/Eudragit L-100 conjugate. Journal of Biotechnology 99:121-131 (2002).
18. Sardar M, Roy I and Gupta MN, Simultaneous purification and immobilization of Aspergillus niger xylanase on the reversibly soluble polymer Eudragit™ L-100. Enzyme and Microbial Technology 27:672-679 (2000).
19. Sharma S, Kaur P, Jain A, Rajeswari MR and Gupta MN, A smart bioconjugate of chymotrypsin. Biomacromolecules 4:330-336 (2003).
20. Silva CJSM and Roberto IC, Optimization of xylitol production by Candida guilliermondii FTI 20 037 using response surface methodology. Process Biochemistry 36:1119-1124 (2001).