Suppression of GABAB receptor function in vivo by disulfide reducing agent, DL-dithiothreitol (DTT)

Psychopharmacology

Volumn 174, Issue 2, 2004, Pages 283-290

  Carai, Mauro A M ^a   Vacca, Giovanni ^a   Serra, Salvatore ^a   Colombo, Giancarlo ^b   Froestl, Wolfgang ^c   Gessa, Gian Luigi ^a,b  

^a UNIVERSITY OF CAGLIARI   (Italy)

^b CNR   (Italy)

^c NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Dithiothreitol (DTT); Baclofen; Discriminative stimulus effects; GABA; Receptor; Sedation hypnosis; Hydroxybutyric acid (GHB)

Indexed keywords

3 AMINOPROPYL(METHYL)PHOSPHINIC ACID; 4 AMINOBUTYRIC ACID B RECEPTOR STIMULATING AGENT; 4 HYDROXYBUTYRIC ACID; 5,5 DIMETHYL 2 MORPHOLINEACETIC ACID; ALCOHOL; BACLOFEN; CGP 44532; DIAZEPAM; DITHIOTHREITOL; KETAMINE; UNCLASSIFIED DRUG;

ANIMAL BEHAVIOR; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DISCRIMINATIVE STIMULUS; DRUG DISCRIMINATION; DRUG EFFECT; DRUG RECEPTOR BINDING; DRUG SPECIFICITY; EVALUATION; MALE; MEDICAL ASSESSMENT; MOUSE; NONHUMAN; PRIORITY JOURNAL; RAT; RAT STRAIN; RECEPTOR BLOCKING; SEDATION; SPECIES COMPARISON;

EID: 3142784465     PISSN: 00333158     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00213-003-1737-y     Document Type: Article

References (25)

1. Aizenman E, Lipton SA, Loring RH (1989) Selective modulation of NMDA responses by reduction and oxidation. Neuron 2:1257-1263
2. Bockaert J, Pin JP (1999) Molecular tinkering of G protein-coupled receptors: an evolutionary success. EMBO J 18:1723-1729
3. Bolser DC, Blythin DJ, Chapman RW, Egan RW, Hey JA, Rizzo C, Kuo S-C, Kreutner W (1995) The pharmacology of SCH 50911: a novel, orally-active GABA-B receptor antagonist. J Pharmacol Exp Ther 274:1393-1398
4. B receptors: first of the functional metabotropic heterodimers. J Pharmacol Exp Ther 292:2-7
5. B receptors: structure and function. Pharmacol Rev 54:247-264
6. B receptors in the sedative/hypnotic effect of γ-hydroxybutyric acid. Eur J Pharmacol 428:315-321
7. B receptors via its conversion into γ-hydroxybutyric acid. Eur J Pharmacol 441:157-163
8. B receptors in the discriminative stimulus effects of γ-hydroxybutyrate in rats: time course and antagonism studies. J Pharmacol Exp Ther 305:668-674
9. Cleland WW (1964) Dithiothreitol, a new protective reagent for SH groups. Biochemistry 3:480-482
10. B receptors in the mediation of discriminative stimulus effects of γ-hydroxybutyric acid. Physiol Behav 64:293-302
11. Colombo G, Lobina C, Agabio R, Brunetti G, Diaz G, Littera M, Melis S, Pani M, Reali R, Serra S, Vacca G, Carai MAM, Gessa GL (2001) Selective breeding of two rat lines differing in sensitivity to GHB and baclofen. Brain Res 902:127-130
12. B receptors: a new paradigm in G protein signaling. Mol Cell Neurosci 16:296-312
13. Dudek BC, Fanelli RJ (1980) Effects of γ-butyrolactone, amphetamine, and haloperidol in mice differing in sensitivity to alcohol. Psychopharmacology 68:87-97
14. B receptor extracellular domain support a Venus flytrap mechanism for ligand binding. J Biol Chem 274:13362-13369
15. Goudie AJ, Leathley MJ (1993) Drug-discrimination assays. In: Sahgal A (ed) Behavioural neuroscience: a practical approach, vol II. Oxford University Press, Oxford, pp 145-167
16. Grant KA, Colombo G (1993) Discriminative stimulus effects of ethanol: effect of training dose on the substitution of N-methyl-D-aspartate antagonists. J Pharmacol Exp Ther 264:1241-1247
17. B receptors uncovers similarity to metabotropic glutamate receptors. Nature 386:239-246
18. Kuriyama K, Taguchi J (1987) Glycoprotein as a constituent of purified γ-aminobutyric acid/benzodiazepine receptor complex: structures and physiological roles of its carbohydrate chain. J Neurochem 48:1897-1903
19. B receptors. Neuropharmacology 38:1667-1673
20. Lobina C, Colombo G, Brunetti G, Diaz G, Melis S, Pani M, Serra S, Vacca G, Cessa GL, Carai MAM (2001) Procedure of bidirectional selective outbreeding for production of two rat lines differing in sensitivity to the sedative/hypnotic effect of γ-hydroxybutyric acid. Brain Res Protoc 8:74-81
21. Marzullo G, Hine B (1980) Opiate receptor function may be modulated through an oxidation-reduction mechanism. Science 208:1171-1173
22. Simon EJ, Groth J (1975) Kinetics of opiate receptor inactivation by sulfhydryl reagents: evidence for conformational change in presence of sodium ions. Proc Natl Acad Sci USA 72:2404-2407
23. Skowronski R, Beaumont K, Fanestil DD (1987) Modification of the peripheral-type benzodiazepine receptor by arachidonate, diethylpyrocarbonate and thiol reagents. Eur J Pharmacol 143:305-314
24. Tabakoff B, Hoffman PL (1987) Biochemical pharmacology of alcohol. In: Meltzer HY (ed) Psychopharmacology: the third generation of progress. Raven Press, New York, pp 1521-1526
25. 1-adrenergic receptors of turkey erythrocyte membranes. Inactivation by dithiothreitol. J Biol Chem 254:4462-4469