Apoptosis: A four-week laboratory investigation for advanced molecular andcellular biology students

Cell Biology Education

Volumn 2, Issue 4, 2003, Pages 275-295

  DiBartolomeis, Susan M ^a   Moné, James P ^a  

^a MILLERSVILLE UNIVERSITY   (United States)

Author keywords

Apoptosis; DNA laddering; Fluorescence microscopy; HL 60 cells; Western blot

Indexed keywords

DNA FRAGMENT;

APOPTOSIS; ARTICLE; CHROMATIN CONDENSATION; CONTROLLED STUDY; CYTOLOGY; DNA DETERMINATION; DNA ISOLATION; EXPERIMENTAL DESIGN; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IMMUNOBLOTTING; LABORATORY TEST; MOLECULAR BIOLOGY; PROTEIN ISOLATION; RESEARCH; SCIENCE; SCIENTIFIC LITERATURE; SIMULATION; STATISTICAL ANALYSIS; STUDENT; WESTERN BLOTTING;

APOPTOSIS; BLOTTING, WESTERN; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CHROMATIN; CURRICULUM; DNA FRAGMENTATION; EDUCATIONAL MEASUREMENT; HUMANS; LEARNING; MOLECULAR BIOLOGY; PROTEINS; RESEARCH; STAINING AND LABELING; STUDENTS; TEACHING; UNIVERSITIES;

EID: 3142740358     PISSN: 15367509     EISSN: None     Source Type: Journal    
DOI: 10.1187/cbe.03-06-0027     Document Type: Article

References (14)

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (2002). Current Protocols in Molecular Biology, New York: John Wiley and Sons.
2. Chang, H.Y., and Yang, X. (2000). Proteases for cell suicide: Functions and regulation of caspases. Micro. Mol. Biol. Rev. 64(4), 821-846.
3. Freshney, R.I. (2000). Culture of Animal Cells: A Manual of Basic Technique, 4th ed., New York: John Wiley and Sons.
4. Hengartner, M.O. (2000). The biochemistry of apoptosis. Nature 407, 770-776.
5. Howard Hughes Medical Institute. (1996). Beyond Bio 101: The Transformation of Undergraduate Biology Education, Chevy Chase, MD: HHMI. Available at http://www.hhmi.org/BeyondBio101.
6. Martin, B.M. (1994). Tissue Culture Techniques, Boston, MA: Birkhauser.
7. Martins, L.M., Kottke, T., Mesner, P.W., Basi, G.S., Sinha, S., Frigon, N., Jr., Tatar, E., Tung, J.S., Bryant, K., Takahashi, A., Svingen, P.A., Madden, B.J., McCormick, D.J., Earnshaw, W.C., and Kaufmann, S.H. (1997). Activation of multiple interleukin-1β converting enzyme homologues in cytosol and nuclei of HL-60 cells during etoposide-induced apoptosis. J. Biol. Chem. 272(11), 7421-7430.
8. National Research Council of the National Academies. (2003). Bio 2010: Transforming Undergraduate Education for Future Research Biologists, Washington, DC: National Academies Press.
9. Odom, D.P., and Grossel, M.J. (2002). Using the two-hybrid screen in the classroom laboratory. Cell Biol. Educ. 1, 43-62.
10. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
11. Smyth, P.G., Berman, S.A., and Bursztajn, S. (2002). Markers of apoptosis: Methods for elucidating the mechanism of apoptotic cell death from the nervous system. Biotechniques 32(3), 648-665.
12. Studzinski, G.P. (1999). Apoptosis: A Practical Approach, New York: Oxford University Press.
13. Wright, R., and Boggs, J. (2002). Learning cell biology as a team: A project-based approach to upper-division cell biology. Cell Biol. Education 1, 145-153.
14. Yoshida, A., Shao, R., and Pommier, Y. (1999). Assessment of DNA damage in apoptosis. In: Apoptosis: A Practical Approach, ed. G.P. Studzinski, New York: Oxford University Press, 41-55.