Discovery and characterization of an antibody, anti-egressin, that is able to inhibit Trypanosoma cruzi egress in vitro

Journal of Parasitology

Volumn 90, Issue 3, 2004, Pages 524-530

  Moore Lai, Deborah ^a,b   Rowland, Edwin ^a  

^a OHIO UNIVERSITY   (United States)

^b HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EGRESSIN ANTIBODY; IMMUNOGLOBULIN G2A; PARASITE ANTIBODY; POLYCLONAL ANTIBODY; UNCLASSIFIED DRUG; ANTIEGRESSIN, TRYPANOSOMA CRUZI; IMMUNOGLOBULIN G;

ANTIBODY; CHAGAS DISEASE; ETIOLOGY; HOST; IMMUNITY; IMMUNOASSAY; INFECTIVITY; INHIBITION; PARASITE; PROTOZOAN; RODENT;

ANTIGEN ANTIBODY REACTION; ARTICLE; CELL DIVISION; CELL MEDIATED CYTOTOXICITY; CHAGAS DISEASE; COMPLEMENT ACTIVATION; DRUG INHIBITION; FIBROBLAST CULTURE; HOST CELL; IMMUNE RESPONSE; IMMUNITY; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; NONHUMAN; OPSONIZATION; PARASITE; PARASITEMIA; QUANTITATIVE ASSAY; SPLEEN CELL; SURVIVAL; TRYPANOSOMA CRUZI; TRYPOMASTIGOTE; VIRUS NEUTRALIZATION; ANIMAL; BAGG ALBINO MOUSE; BLOOD; CELL LINE; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; FIBROBLAST; FRACTIONATION; IMMUNOHISTOCHEMISTRY; IMMUNOLOGY; METHODOLOGY; MOLECULAR WEIGHT; MOUSE; PARASITOLOGY;

PROTOZOA; TRYPANOSOMA CRUZI;

ANIMALS; CELL LINE; CHEMICAL FRACTIONATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; FIBROBLASTS; IMMUNOGLOBULIN G; IMMUNOHISTOCHEMISTRY; MICE; MICE, INBRED BALB C; MOLECULAR WEIGHT; TRYPANOSOMA CRUZI;

EID: 3142677753     PISSN: 00223395     EISSN: None     Source Type: Journal    
DOI: 10.1645/GE-145R     Document Type: Article

References (30)

1. ALIBERTI, J. C. S., M. A. G. CARDOSO, G. A. MARTINS, R. T. GAZZINELLI, L. Q. VIERIA, AND J. S. SILVA. 1996. IL-12 mediates resistance to Trypanosoma cruzi in mice and is produced by murine macrophages in response to live trypomastigotes. Infection and Immunity 64: 1961-1967.
2. ALMEIDA, I. C., M. A. J. FERGUSON, S. SCHENKMAN, AND L. R. TRAVASSOS. 1994. Lytic anti-a-galactosyl antibodies from patients with chronic Chagas disease recognize novel O-linked oligosaccharides on mucin-like glycosylphosphatidylinositol-anchored glycoproteins of Trypanosoma cruzi. Journal of Biochemistry 304: 793-802.
3. BRENER, Z., AND R. T. GAZZINELLI. 1997. Immunological control of Trypanosoma cruzi infection and pathogenesis of Chagas disease. International Archives of Allergy and Immunology 114: 103-110.
4. BRODSKYN, C. I., A. M. M. SILVA, H. A. TAKEHARA, AND I. MOTA. 1989. IgG subclasses responsible for immune clearance in mice infected with Trypanosoma cruzi. Immunology and Cellular Biology 67: 343-348.
5. BUCKNER, F. S., AND W. C. V. VOORHIS. 2000. Immune response to Trypanosoma cruzi: Control of infection and pathogenesis of Chagas' disease. In Effects of microbes on the immune system, M. W. Cunningham and R. S. Fujinami (eds.). Lippincott Williams & Wilkins, Philadelphia, Pennsylvania, p. 569-592.
6. D'IMPERIO, M. R. L., H. EISEN, P. MINOPRIO, M. JOSKOWICZ, AND A. COUTINHO. 1986. Persistence of polyclonal B cell activation with undetectable parasitemia in late stages of experimental Chagas disease. Journal of Immunology 127: 353-356.
7. -, M. JOSKOWICZ, A. COUTINHO, T. KIPNIS, AND H. EISEN. 1985. Very large and isotypically atypical polyclonal plaque-forming cell responses in mice infected with Trypanosoma cruzi. European Journal of Immunology 15: 201-203.
8. ELHOUHDIDI, A., C. TRUYENS, M. T. RIVERA, H. HAZIN, AND Y. CARLIER. 1994. Trypanosoma cruzi infection in mice induces a polyisotypic hypergammaglobulinemia and parasite-specific response involving high IgG2a concentrations and highly avid IgG1 antibodies. Parasite Immunology 16: 69-76.
9. GAZZINELLI, R. T., S. HIENY, T. A. WYNN, S. WOLF, AND A. SHER. 1993. IL-12 is required for the T-lymphocyte-independent induction of IFN-γ by an intracellular parasite and induces resistance in T-cell deficient hosts. Proceedings of the National Academy of Science of the United States of America 90: 6115-6119.
10. HEINZEL, F. P. 1995. Antibodies. In Principles and practice of infectious diseases, G. L. Mandell, J. E. Bennett, and R. Dolin (eds.). Churchill Livingstone, New York, p. 36-57.
11. HINDS, L. B., M. S. ALEXANDRE-MOREIRA, D. DECOTE-RICARDO, M. P. NUNES, AND L. M. T. PECANHA. 2001. Increased immunoglobulin secretion by B lymphocytes from T. cruzi-infected mice after B lymphocyte-NK cell interaction. Parasite Immunology 23: 581-586.
12. KRETTLI, A. U., AND Z. BRENER. 1976. Protective effects of specific antibodies in Trypanosoma cruzi infections. Journal of Immunology 116: 755-760.
13. LIMA-MARTINS, M. V. C., G. A. SANCHEZ, A. U. KRETTLI, AND Z. BRENER. 1985. Antibody-dependent cell cytotoxicity against Trypanosoma cruzi is only mediated by protective antibodies. Parasite Immunology 7: 367-376.
14. MARCIPAR, I. S., M. G. RISSO, A. M. SIBER, S. REVELLI, AND A. J. MARCIPAR. 2001. Antibody maturation in T. cruzi-infected rats. Clinical and Diagnostic Laboratory Immunology 8: 802-805.
15. MARKINE-GORIAYNOFF, D., T. M. DOMINIQUE, C. T. LOGT, T. D. NGUYEN, F. W. A. HESSEN, G. BIGAIGNON, Y. CARLIER, AND J. P. COUTELIER. 2000. IFN-gamma-independent IgG2a production in mice infected with viruses and parasites. International Immunology 12: 223-230.
16. MINOPRIO, P., H. EISEN, L. FORNI, L. M. R. D'IMPERIO, M. JOSKOWICZ, AND A. COUTINHO. 1986. A polyclonal lymphocyte response to murine T. cruzi infection, I. Quantitation of both T and B responses. Scandinavian Journal of Immunology 24: 661-668.
17. -, -, M. JOSKOWICZ, P. PEREIRA, AND A. COUTINHO. 1987. Suppression of polyclonal antibody production in Trypanosoma cruzi-infected mice by treatment with anti-L3T4 antibodies. Journal of Immunology 139: 545-550.
18. MOORE-LAI, D., AND E. C. ROWLAND. 2004. Anti-egressin acts late in the intracellular growth cycle of Trypanosoma cruzi to inhibit parasite egress. Journal of Parasitology 90: 85-91.
19. MOSMANN, T. R., S. SAD, AND D. KAGI. 1996. Perform and fas killing by CD8+ T cells limits their cytokine synthesis and proliferation. Journal of Experimental Medicine 184: 1543-1547.
20. MOTA, I., AND L. F. UMEKITA. 1989. The effect of C3 depletion on the clearance of Trypanosoma cruzi induced by IgG antibodies. Immunology Letters 21: 223-226.
21. NASH, P. B., M. B. PURNER, R. P. LEON, P. CLARKE, R. C. DUKE, AND T. J. CURIEL. 1998. Toxoplasma gondii-infected cells are resistant to multiple inducers of apoptosis. Journal of Immunology 160: 1824-1830.
22. ORTIZ-ORTIZ, L., T. ORTEGA, R. CAPIN, AND T. MARTINEZ. 1976. Enhanced mononuclear phagocytic activity during Trypanosoma cruzi infection in mice. International Archives of Allergy and Applied Immunology 50: 232-242.
23. PAUL, W. E. 1999. Fundamental immunology, 4th ed. Lippincott-Raven Publishers, Philadelphia, Pennsylvania, 837 p.
24. ROTTENBERG, M. E., R. CARDONI, R. ANDERSSON, E. SEGURE, AND A. ORN. 1988. Role of T-helper inducer cells as well as NK cells in resistance to T. cruzi infection. Scandinavian Journal of Immunology 28: 573-582.
25. ROWLAND, E. C., AND Z. CHEN. 2003. Inhibition of Trypanosoma cruzi egress from infected fibroblasts is mediated by CD4+ and μ+ immune cells. Journal of Parasitology 89: 733-737.
26. -, K. S. MIKHAIL, AND T. S. MCCORMICK. 1992. Isotype determination of anti-Trypanosoma cruzi antibodies in murine Chagas disease. Journal of Parasitology 78: 557-561.
27. SCOTT, M. T., AND L. MOYES. 1982. 75Se-methionine labeled Trypanosoma cruzi blood trypomastigotes: Opsonization by chronic infection serum facilitates killing in spleen and liver. Clinical and Experimental Immunology 48: 754-757.
28. SILVA, M. T., P. J. MORRISSEY, K. H. GRABSTEIN, K. M. MOHLER, D. ANDERSON, AND S. G. REED. 1992. Interleukin 10 and Interferon g regulation of experimental Trypanosoma cruzi infection. Journal of Experimental Medicine 175: 169-174.
29. SPINELLA, S., P. LIEGEARD, AND M. HONTEBEYRIEJOSKOWICZ. 1992. T. cruzi predominance of IgG2a in a nonspecific humoral response during experimental Chagas disease. Experimental Parasitology 74: 46-56.
30. WORLD HEALTH ORGANIZATION. 2002. Control of Chagas disease. Technical report series 905. World Health Organization, Geneva, Switzerland, 109 p.