Chemical modification of papain and subtilisin: An active site comparison

Journal of Chemical Education

Volumn 81, Issue 7, 2004, Pages 1048-1050

  St Vincent, Mireille ^a   Dickman, Michael ^a  

^a University Saint Boniface   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL MODIFICATION; ENZYME INHIBITION; ENZYME KINETICS; ENZYMES; EXPERIMENTS; STUDENTS; TEACHING;

ENZYME CHEMISTRY; METHYL METHANETHIOSULFONATE; PAPAIN; PHENYLMETHYLSULFONYL FLUORIDE; SUBTILISIN;

BIOCHEMISTRY;

EID: 3142672129     PISSN: 00219584     EISSN: None     Source Type: Journal    
DOI: 10.1021/ed081p1048     Document Type: Article

References (9)

1. Fersht, A. Structure and Mechanism in Protein Science; Freeman: New York, 1999; Chapter 9.
2. Columbus, L.; Hubbell, W. L. TiBS, 2002, 27, 288-295.
3. Singh, R.; Blättler, W. A.; Collinson, A. R. Methods Enzymol. 1995, 251, 229-236.
4. Gold, A. M.; Fahrney, D. Biochemistry 1964, 3, 783-791.
5. Whitaker, J. R.; Perez-Villasenor, J. Arch. Biochem. Biophys. 1968, 124, 70-78.
6. Klein, I. B.; Kirsch, J. F. Biochem. Biophys. Res. Commun. 1969, 34, 575-581.
7. Singh, R.; Blättler, W. A.; Collinson, A. R. Anal. Biochem. 1993, 213, 49-56.
8. Roberts, D. D.; Lewis, S. D.; Ballou, D. P.; Olson, S. T.; Shafer, J. A. Biochemistry 1986, 25, 5595-5601.
9. Hsia, C. Y.; Ganshaw, G.; Paech, C.; Murray, C. J. Anal. Biochem. 1996, 242, 221-227.