Nonspecific enhancement of gene expression by compounds identified in high-throughput cell-based screening

BioTechniques

Volumn 37, Issue 1, 2004, Pages 120-122

  Cunningham, Steven C ^a   Ryu, Byungwoo ^a   Sohn, Taylor A ^a   Kern, Scott E ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

1 PHENYL 3 (2 THIAZOLYL)THIOUREA; 2 METHYL 4 PHENYLPYRIDO[1,2 A]PYRIMIDIN 5 YLIUM; 3 [2 HYDRAZONO 2 (4 TOLYL)ETHYLIDENE] 3,4 DIHYDRO 1H QUINOXALIN 2 ONE; 4 [(4 BENZOTHIAZOL 2 YLPHENYLIMINO)METHYL]PHENOL; 5 (4 HYDROXYBENZYLIDENE) 3 (4 HYDROXYPHENYL) 2 THIOXOTHIAZOLIDIN 4 ONE; 5 BENZENESULFONYLAMINO 2 METHYL 1 PHENYL 1H INDOLE 3 CARBOXYLIC ACID ETHYL ESTER; ACRIDIN 9 YL [4 (4 METHYLPIPERAZIN 1 YL)PHENYL]AMINE; APIGENIN; BENZO[G]QUINOLIN 4 YL (1 ETHYLPIPERIDIN 3 YL)AMINE; DRUG; HYDRALAZINE; MEPACRINE; N4 BENZO[G]QUINOLIN 4 YL N1,N1 DIETHYLPENTANE 1,4 DIAMINE; PIPERINE; PYRIMIDINE DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; DRUG POTENTIATION; DRUG SCREENING; ENHANCER REGION; GENE ACTIVATION; GENE EXPRESSION REGULATION; GENETIC TRANSFECTION; HIGH THROUGHPUT SCREENING; REPORTER GENE;

EID: 3142655243     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/04371dd01     Document Type: Article

References (15)

1. Jensen, L.E. and A.S. Whitehead. 2003. ELAM-1/E-selectin promoter contains an inducible AP-1/CREB site and is not NF-kappa B-specific. BioTechniques 35:54-58.
2. Hong, S.J., H. Chae, and K.S. Kim. 2002. Promoterless luciferase reporter gene is transactivated by basic helix-loop-helix transcription factors. BioTechniques 33:1236-1240.
3. Osborne, S.A. and K.F. Tonissen. 2002. pRL-TK induction can cause misinterpretation of gene promoter activity. BioTechniques 33:1240-1242.
4. Annicotte, J.S., K. Schoonjans, C. Haby, and J. Auwerx. 2001. An E-box in pGL3 reporter vectors precludes their use for the study of sterol regulatory element-binding proteins. BioTechniques 31:993-996.
5. McGovern, S.L., E. Caselli, N. Grigorieff, and B.K. Shoichet. 2002. A common mechanism underlying promiscuous inhibitors from virtual and high-throughput screening. J. Med. Chem. 45:1712-1722.
6. Su, G.H., T.A. Sohn, B. Ryu, and S.E. Kern. 2000. A novel histone deacetylase inhibitor identified by high-throughput transcriptional screening of a compound library. Cancer Res. 60:3137-3142.
7. Yoshida, M., M. Kijima, M. Akita, and T. Beppu. 1990. Potent and specific inhibition of mammalian histone deacetylase both in vivo and in vitro by trichostatin A. J. Biol. Chem. 265:17174-17179.
8. Sohn, T.A., R. Bansal, G.H. Su, K.M. Murphy, and S.E. Kern. 2002. High-throughput measurement of the Tp53 response to anticancer drugs and random compounds using a stably integrated Tp53-responsive luciferase reporter. Carcinogenesis 23:949-957.
9. Mulholland, N.M., E. Soeth, and C.L. Smith. 2003. Inhibition of MMTV transcription by HDAC inhibitors occurs independent of changes in chromatin remodeling and increased historic acetylation. Oncogene 22:4807-4818.
10. Kelly, W.K., V.M. Richon, O. O'Connor, T. Curley, B. MacGregor-Curtelli, W. Tong, M. Klang, L. Schwartz, et al. 2003. Phase I clinical trial of histone deacetylase inhibitor: suberoylanilide hydroxamic acid administered intravenously. Clin. Cancer Res. 9:3578-3588.
11. Wang, W. and W.S. El-Deiry. 2003. Bioluminescent molecular imaging of endogenous and exogenous p53-mediated transcription in vitro and in vivo using an HCT116 human colon carcinoma xenograft model. Cancer Biol. Ther. 2:196-202.
12. Torrance, C.J., V. Agrawal, B. Vogelstein, and K.W. Kinzler. 2001. Use of isogenic human cancer cells for high-throughput screening and drug discovery. Nat. Biotechnol. 19:940-945.
13. Gossen, M. and H. Bujard. 1992. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. USA 89:5547-5551.
14. Taipale, J., J.K. Chen, M.K. Cooper, B. Wang, R.K. Mann, L. Milenkovic, M.P. Scott, and P.A. Beachy. 2000. Effects of oncogenic mutations in Smoothened and Patched can be reversed by cyclopamine. Nature 406:1005-1009.
15. Wilson, V.S., K. Bobseine, C.R. Lambright, and L.E. Gray, Jr. 2002. A novel cell line, MDA-kb2, that stably expresses an androgen- and glucocorticoid-responsive reporter for the detection of hormone receptor agonists and antagonists. Toxicol. Sci. 66:69-81.