Expression, purification, and C-terminal amidation of recombinant human glucagon-like peptide-1

Protein Expression and Purification

Volumn 36, Issue 2, 2004, Pages 292-299

  Zhang, Zhi Zhen ^a,b   Yang, Sheng Sheng ^c   Dou, Hong ^d   Mao, Ji Fang ^c   Li, Kang Sheng ^a  

^a SHANTOU UNIVERSITY MEDICAL COLLEGE   (China)

^b SHANXI UNIVERSITY   (China)

^c SECOND MILITARY MEDICAL UNIVERSITY   (China)

^d University of Texas Medical Branch School of Medicine   (United States)

Author keywords

Fusion protein expression; Purification; Recombinant human glucagon like peptide 1; Transacylation

Indexed keywords

ARGININE; CARBOXYPEPTIDASE C; CYANOGEN BROMIDE; GLUCAGON; GLUCAGON LIKE PEPTIDE 1; GLUCOSE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; INSULIN; LEUCINE; PEPTIDE FRAGMENT; PROTEIN PRECURSOR;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; BLOOD; CHEMISTRY; ESCHERICHIA COLI; FEMALE; GENE EXPRESSION; GENETICS; HUMAN; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; NON INSULIN DEPENDENT DIABETES MELLITUS; RAT; SPRAGUE DAWLEY RAT;

ANIMALS; ARGININE; CARBOXYPEPTIDASE C; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, ION EXCHANGE; CYANOGEN BROMIDE; DIABETES MELLITUS, TYPE 2; ESCHERICHIA COLI; FEMALE; GENE EXPRESSION; GLUCAGON; GLUCAGON-LIKE PEPTIDE 1; GLUCOSE; GLUTATHIONE TRANSFERASE; HUMANS; INSULIN; LEUCINE; PEPTIDE FRAGMENTS; PROTEIN PRECURSORS; RATS; RATS, SPRAGUE-DAWLEY; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 3142647790     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.03.014     Document Type: Article

References (26)

1. Ørskov C., Holst J.J., Seier-Poulsen S., Kirkegaard P. Pancreatic and intestinal processing of proglucagon in man. Diabetologia. 30:1986;874-881
2. Ørskov C., Rabenhoj L., Kofod H., Wettergern A., Holst J.J. Production and secretion of amidated and glycine-extended glucagon-like peptide-1 (GLP-1) in man. Diabetes. 43:1994;535-539
3. Dachicourt N., Serradas P., Bailbé D., Kergoat M., Doaré L., Portha B. Glucagon-like peptide-1 (7-36)-amide confers glucose sensitivity to previously glucose-incompetent β-cells in diabetic rats: in vivo and in vitro studies. J. Endocrinol. 155:1997;369-376
4. Radosavljevic T., Todorovic V., Sikic B. Role of glucagon-like peptide 1 (GLP-1) in regulation of insulin secretion. Srp. Arh. Celok. Lek. 130:2002;416-419
5. Kreymann B., Williams G., Ghattei M.A., Bloom S.R. Glucagon-like peptide-1 7-36: a physiological incretin in man. Lancet II. 1987;1300-1304
6. Nauck M.A., Niedereichholz U., Ettler R. Glucagon-like peptide 1 inhibition of gastric emptying outweighs its insulinotropic effects in healthy humans. Am. J. Physiol. 273(5 Pt. 1):1997;E981-988
7. Gutniak M., Ørskov C., Holst J.J., Ahren B., Efendic S. Antidiabetic effect of glucagon-like peptide-1 [7-36] amide in normal subjects and patients with diabetes. N. Engl. J. Med. 326:1992;1316-1322
8. Willms B., Werner J., Holst J.J., Ørskov C., Creutzfeldt W., Nauck M.A. Gastric emptying, glucose responses, and insulin secretion after a liquid test meal: effects of exogenous glucagon-like peptide-1 (GLP-1)-(7-36) amide in type 2 (noninsulin-dependent) diabetic patients. J. Clin. Endocrinol. Metab. 81:1996;327-332
9. Nauck M.A., Holst J.J., Willms B., Schmiegel W. Glucagon-like peptide-1 (GLP-1) as a new therapeutic approach for type 2-diabetes. Exp. Clin. Endocrinol. Diabetes. 105:1997;187-195
10. Holst J.J. Glucagon-like peptide-1, a gastrointestinal hormone with a pharmaceutical potential. Curr. Med. Chem. 6:1999;1005-1017
11. Drucker D.J. Biological actions and therapeutic potential of the glucagon-like peptides. Gastroenterology. 122:2002;531-544
12. Ahren B. Gut peptides and type 2 diabetes mellitus treatment. Curr. Diabetes Rep. 3:2003;365-372
13. Deacon C.F., Johnsen A.H., Holst J.J. Degradation of glucagon-like peptide-1 by human plasma in vitro yields an N-terminally truncated peptide that is a major endogenous metabolite in vivo. J. Clin. Endocrinol. Metab. 80:1995;952-957
14. Ahren B., Holst J.J., Mari A. Characterization of GLP-1 effects on beta-cell function after meal ingestion in humans. Diabetes Care. 26:2003;2860-2864
15. Suzuki K., Shimoi H., Iwasaki Y., Kawahrra T., Matsuura Y., Nishikawa Y. Elucidation of amidating reaction mechanism by frog amidating enzyme, peptidylglycine alpha-hydroxylating monooxygenase, expressed in insect cell culture. EMBO J. 9:1990;4259-4265
16. Breddam K., Widmer F., Meldal M. Amidation of growth hormone releasing factor (1-29) by serine carboxypeptidase catalysed transpeptidation. Int. J. Pept. Protein Res. 37:1991;153-160
17. Zhang Z.Z., Yang S.S., Mao J.F. Purification and bioactivity of recombinant human glucagons-like peptide-1. Chin. J. Biochem. Mol. Biol. 18:2002;5-8
18. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning. A Laboratory Manual. second ed. 1993;325-332 Science Press, Beijing
19. Holst J.J., Bersani M., Johnsen A.H., Kofod H., Hartmann B., Ørskov C. Proglucagon processing in porcine and human pancreas. J. Biol. Chem. 269:1994;18827-18833
20. Kavianipour M., Ehlers M.R., Malmberg K., Ronquist G., Ryden L., Wikstrom G., Gutniak M. Glucagon-like peptide-1 (7-36) amide prevents the accumulation of pyruvate and lactate in the ischemic and non-ischemic porcine myocardium. Peptides. 24:2003;569-578
21. Nauck M.A., Meier J.J., Creutzfeldt W. Incretins and their analogues as new antidiabetic drugs. Drug News Perspect. 16:2003;413-422
22. Stratton J., Chiruvolu V., Meagher M. High cell-density fermentation. Methods Mol. Biol. 103:1998;107-120
23. Domingues L., Lima N., Teixeira J.A. Contamination of high-cell-density continuous bioreactor. Biotechnol. Bioeng. 68:2000;584-587
24. Gallwitz B., Ropeter T., Morys-Wortman C., Mentlein R., Sieyel E.G., Schmidt W.E. Glp-1-analogues resistant to degradation by dipeptidyl peptidase IV in vitro. Regul. Pept. 86:2000;103-111
25. Xiao Q., Giguere J., Parisien M., Jeng W., St-Pierre S.A., Brubaker P.L., Wheeler M.B. Biological activities of glucagon-like peptide-1 analogues in vitro and in vivo. Biochemistry. 40:2001;2860-2869
26. Parkes D.G., Pittner R., Jodka C., Smith P., Young A. Insulinotropic actions of exendin-4 and glucagon-like peptide-1 in vivo and in vitro. Metabolism. 50:2001;583-589