Expression, purification, and kinetic constants for human and Escherichia coli pyridoxal kinases

Protein Expression and Purification

Volumn 36, Issue 2, 2004, Pages 300-306

  Di Salvo, Martino L ^a   Hunt, Sharyn ^b   Schirch, Verne ^b  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

^b VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

Author keywords

Pyridoxal kinase; Pyridoxal phosphate

Indexed keywords

ADENOSINE TRIPHOSPHATE; AMINO ACID; ESCHERICHIA COLI PROTEIN; PYRIDOXAL KINASE; PYRIDOXINE; RECOMBINANT PROTEIN;

ARTICLE; CARBOHYDRATE METABOLISM; CHEMISTRY; COENZYME; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; PHYSIOLOGY; PLASMID;

ADENOSINE TRIPHOSPHATE; AMINO ACIDS; CARBOHYDRATE METABOLISM; COENZYMES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; HUMANS; METABOLISM; PLASMIDS; PYRIDOXAL KINASE; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; VITAMIN B 6;

ESCHERICHIA COLI;

EID: 3142531317     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.04.021     Document Type: Article

References (24)

1. Sivaraman J., Li Y., Banks J., Cane D.E., Matte A., Cygler M. Crystal structure of Escherichia coli PdxA, and an enzyme involved in the pyridoxal phosphate biosynthesis pathway. J. Biol. Chem. 278:2003;43682-43690
2. Dempsey W.B. Synthesis of pyridoxal phosphate. Neidhardt F.C., Ingraham J.L., Los K.B., Magasanik B., Schaechter M., Umbarger H.E. Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology. 1980;539-543 American Society for Microbiology, Washington, DC
3. 6 and Pyridoxal Phosphate, Part A. vol. 1:1986;417-476 Wiley Interscience, New York
4. 6) kinase in Escherichia coli K-12. FEMS Microbiol. Lett. 141:1996;89-95
5. ′ -phosphate biosynthesis in Escherichia coli K-12 J. Bacteriol. 180:1998;1814-1821
6. Maras B., Valiante S., Orru S., Simmaco M., Barra D., Churchich J.E. Structure of pyridoxal kinase from sheep brain and role of the tryptophanyl residues. J. Protein Chem. 8:1999;259-268
7. Gao Z.G., Lau C.K., Lo S.C., Choi S.Y., Churchich J.E., Kwok F. Porcine pyridoxal kinase cDNA cloning, expression and confirmation of its primary sequence. Int. J. Biochem. Cell Biol. 12:1998;1379-1388
8. Hanna M.C., Turner A.J., Kirkness E.F. Human pyridoxal kinase: cDNA cloning, expression, and modulation by ligands of the benzodiazepine receptor. J. Biol. Chem. 272:1997;10756-10760
9. Gao G.L., Fonda M.L. Evidence for a phosphoenzyme intermediate formed during catalysis by pyridoxal phosphatase from human erythrocytes. Arch. Biochem. Biophys. 313:1994;166-172
10. ′ -phosphate oxidase J. Mol. Biol. 315:2002;385-397
11. ′ -phosphate oxidase Protein Sci. 12:2003;1455-1463
12. Lee H.-S., Moon B.J., Choi S.Y., Kwon O.-S. Human pyridoxal kinase: overexpression and properties of the recombinant enzyme. Mol. Cells. 10:2000;452-459
13. Scott T.C., Phillips M.A. characterization of Trypanosoma brucei pyridoxal kinase: purification, gene isolation and expression in Escherichia coli. Mol. Biochem. Parasitol. 88:1997;1-11
14. Lum H.K., Kwok F., Lo S.C. Cloning and characterization of Arabidopsis thaliana pyridoxal kinase. Planta. 215:2002;870-879
15. Li M.H., Kwok F., Chang W.R., Lau C.K., Zhang J.P., Liu S.O., Leung Y.C., Jiang T., Liang D.C. Crystal structure of brain pyridoxal kinase, a novel member of the ribokinase superfamily. J. Biol. Chem. 277:2002;46385-46390
16. Li M.-H., Kwok F., Chang W.-R., Liu S.-Q., Lo S.C.L., Zhang J.-P., Jiang T., Liang D.-C. Conformational changes in the reaction of pyridoxal kinase. J. Biol. Chem. 279:2004;17459-17465
17. Fu T.-F., di Salvo M., Schirch V. Enzymatic determination of homocysteine in cell extracts. Anal. Biochem. 290:2001;359-365
18. Layne E. Spectrophotometric and turbidimetric methods for measuring protein. Kaplan N.O. Methods in Enzymology. vol. III:1957;447-454 Academic Press, New York
19. Fu T.-F., di Salvo M., Schirch V. Distribution of B6 vitamers in Escherichia coli as determined by enzymatic assay. Anal. Biochem. 298:2001;314-321
20. Gill S.C., von Hippel P.H. Calculation of protein extinction coefficients from amino acid sequence data. Anal. Biochem. 182:1989;219-236
21. 6 kinase from Escherichia coli B. Biochemistry. 9:1970;4070-4074
22. McCormick D.B., Gregory M.E., Snell E.E. Pyridoxal phosphokinases: I. assay, distribution, purification, and properties. J. Biol. Chem. 236:1961;2076-2084
23. Kwok F., Churchich J.E. Brain pyridoxal kinase: purification, substrate specificities, and sensitized photodestruction of an essential histidine. J. Biol. Chem. 254:1979;6489-6495
24. Fong C.-C., Lai W.-P., Leung Y.-C., Lo C.-L., Wong M.-S., Yang M. Study of substrate-enzyme interaction between immobilized pyridoxamine and recombinant porcine pyridoxal kinase using surface plasmon resonance biosensor. Biochim. Biophys. Acta. 1596:2002;95-107