Study of substrate-enzyme interaction between immobilized pyridoxamine and recombinant porcine pyridoxal kinase using surface plasmon resonance biosensor

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1596, Issue 1, 2002, Pages 95-107

  Fong, Chi Chun ^a   Lai, Wan Ping ^b   Leung, Yun Chung ^b   Lo, Samuel C L ^b   Wong, Man Sau ^b   Yang, Mengsu ^a  

^a CITY UNIVERSITY OF HONG KONG   (Hong Kong)

^b HONG KONG POLYTECHNIC UNIVERSITY   (Hong Kong)

Author keywords

Pyridoxal kinese; Self assembled monolayer; SPR biosensor

Indexed keywords

11 MERCAPTOUNDECANIC ACID; ACID; BUFFER; MAGNESIUM ION; MANGANESE; POTASSIUM ION; PYRIDOXAL; PYRIDOXAL 5 PHOSPHATE; PYRIDOXAL KINASE; PYRIDOXAMINE; PYRIDOXINE; SODIUM ION; UNCLASSIFIED DRUG; ZINC ION;

ACIDITY; ARTICLE; BINDING AFFINITY; BINDING COMPETITION; BINDING KINETICS; ENZYME BINDING; ENZYME SUBSTRATE COMPLEX; ENZYMIC BIOSENSOR; NONLINEAR SYSTEM; PRIORITY JOURNAL; PROTEIN IMMOBILIZATION; SURFACE PLASMON RESONANCE;

ANIMALS; BINDING, COMPETITIVE; CATIONS, DIVALENT; CATIONS, MONOVALENT; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; KINETICS; PYRIDOXAL KINASE; PYRIDOXAMINE; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; SURFACE PLASMON RESONANCE; SWINE; THERMODYNAMICS;

SUIDAE;

EID: 0036538611     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(02)00208-X     Document Type: Article

References (31)