Expression, purification, and structural analysis of HISUBE2G2 (human ubiquitin-conjugating enzyme)

Protein Expression and Purification

Volumn 45, Issue 2, 2006, Pages 324-328

  Reyes, Luis F ^a   Sommer, César A ^a   Beltramini, Leila M ^b   Henrique Silva, Flávio ^a  

^a FEDERAL UNIVERSITY OF SÃO CARLOS   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Affinity purification; Chromosome 21; Circular dichroism; Class I E2s; HIS tag; Human UBE2G2 UBC7; Ubiquitin system; Ubiquitin binding assay

Indexed keywords

RECOMBINANT PROTEIN; UBE2G2 PROTEIN, HUMAN; UBIQUITIN CONJUGATING ENZYME;

ARTICLE; CHEMISTRY; CIRCULAR DICHROISM; ESCHERICHIA COLI; GENETICS; HELA CELL; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; OPEN READING FRAME; PROTEIN SECONDARY STRUCTURE;

CIRCULAR DICHROISM; CLONING, MOLECULAR; ESCHERICHIA COLI; HELA CELLS; HUMANS; OPEN READING FRAMES; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; UBIQUITIN-CONJUGATING ENZYMES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA;

EID: 31144444775     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.08.018     Document Type: Article

References (31)

1. M. Hochstrasser Ubiquitin-dependent protein degradation Annu. Rev. Genet. 30 1996 405 440
2. A.M. Weissman Themes and variations on ubiquitylation Nat. Rev. Mol. Cell Biol. 3 2001 169 178
3. A. Hershko, H.S. Heller, and C. Ciechanover Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown J. Biol. Chem. 258 13 1983 8206 8214
4. A. Ciechanover, and A.L. Schuartz The ubiquitin system: pathogenesis of human diseases and drug targeting Biochim. Biophys. Acta 29 1695(1-3) 2004 3 17
5. A.L. Haas, and T.J. Siepmann Pathways of ubiquitin conjugation FASEB J. 11 14 1997 1257 1268
6. T.J. Siepmann, R.N. Bohnsack, Z. Tokgo, O.V. Baboshina, and A.L. Haas Protein interactions within the N-end rule ubiquitin ligation pathway J. Biol. Chem. 278 14 2003 9448 9457
7. C.M. Pickart Mechanisms underlying ubiquitination Annu. Rev. Biochem. 70 2001 503 533
8. V. Chau, J.W. Tobias, A. Bachmair, D. Marriot, D.J. Ecker, D.K. Gonda, and A. Varshavsky A multiubiquitin chain is confined to specific lysine in a targeted short-lived protein Science 243 1989 1576 1583
9. K. Matuschewski, H.P. Hauser, M. Treier, and S. Jentsch Identification of a novel family of ubiquitin-conjugating enzymes with distinct amino-terminal extensions J. Biol. Chem. 271 1996 2789 2794
10. W.J. Cook, L.C Jeffrey, Y. Xu, and V. Chau Tertiary structures of class I ubiquitin-conjugating enzymes are highly conserved: crystal structure of yeast Ubc4 Biochemistry 32 50 1993 13809 13817
11. W.J. Cook, P.D. Martin, B.F. Edwards, R.K. Yamazaki, and V. Chau Crystal structure of a class I ubiquitin conjugatin enzyme (UBC7) from Saccharomyces cerevisiae at 2.9 amstrongs resolution Biochemistry 36 1997 1621 1627
12. T.V.C Biederer, and T. Sommer Degradation of subunits of the Sec61p complex, an integral component of the ER membrane, by the ubiquitin-proteasome pathway EMBO J. 15 9 1996 2069 2076
13. S.A. Rose, J.P. Leek, T.P. Moynihan, H.C. Ardley, A.F. Markham, and P.A. Robinson Assignment of the ubiquitin conjugating enzyme gene, UBE2G2, to human chromosome band 21q22.3 by in situ hybridization Cytogenet. Cell Genet. 83 1-2 1998 98 99
14. N. Katsanis, and E.M.C. Fisher Identification, expression, and chromosomal localization of ubiquitin conjugating enzyme 7 (UBE2G2), a human homologue of the Saccharomyces cerevisiae UBE2G2 Gene. Genomics. 51 1998 128 131
15. J. Jungmann, H. Reins, and S. Jentsch Resistance to cadmium mediated by ubiquitin-dependent proteolysis Nature 361 1993 369 371
16. R.K. Yamazaky, and V. Chau Bacterial expression of the Saccharomyces cereviae ubiquitin-conjugating enzyme UBC7 Protein Expr. Purif. 7 1996 122 127
17. S. Tiwari, and A.M. Weissman Endoplasmic reticulumun (ER)-associated degradation of T cell receptor subunits, involvement of ER-associated ubiquitin-conjugating enzymes (E2s) J. Biol. Chem. 276 2001 16200 16913
18. S. Fang, M. Ferrone, C. Yang, J.P. Jensen, S. Tiwari, and A.M. Weissman The tumor autocrine motility factor receptor, gp 78, is a ubiquitin protein ligase implicated in the degradation from the endoplasmic reticulum PNAS 98 2001 14422 14427
19. 2+ J. Biol. Chem. 278 2003 38238 38246
20. B.W. Kim, A.M. Zavacki, C. Curcio-Morelli, M. Dentice, J.M. Harney, P.R. Larsen, and A.C. Bianco Endoplasmic reticulum-associated degradation of the human type 2-iodothyronine deiodinase (D2) is mediated via an association between mammalian UBC7 and the carboxyl region of D2 Mol. Endocrinol. 17 12 2003 2603 2612
21. M. Kikkert, R. Doolman, M. Dai, R. Avner, G. Hassink, S. van Voorden, S. Thanedar, J. Roitelman, V. Chau, and E. Wiertz Human HRD1 is an E3 ubiquitin ligase involved in degradation of proteins from the endoplasmic reticulum J. Biol. Chem. 279 5 2004 3525 3534
22. G. Hassink, M. Kikkert, S. van Voorden, S.J. Lee, R. Spaapen, T. van Laar, C.S. Coleman, E. Bartee, K. Fruh, V. Chau, and E.J. Wiertz TEB4 is a C4HC3 RING finger-containing ubiquitin ligase of the endoplasmic reticulum Biochem. J. 2005 [Epub ahead of print]
23. M. Hakli, K.L. Lorick, A.M. Weissman, O.A. Janne, and J.J. Palvimo Transcriptional coregulator SNURF (RNF4) possesses ubiquitin E3 ligase activity FEBS Lett. 560 1-3 2004 56 62
24. Q. Liu, F. Shang, W. Guo, M. Hobbs, P. Valverde, V. Reddy, and A. Taylor Regulation of the ubiquitin proteasome pathway in human lens epithelial cells during the cell cycle Exp. Eye Res. 78 2 2004 197 205
25. M.M. Bradford Rapid and sensitive for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 1976 248 254
26. N. Sreerama, and R.W. Woody A self-consistent method for the analysis of proteins secondary structure form circular dichroism Anal. Biochem. 209 1993 32 44
27. H. Tong, G. Hateboer, A. Perrakis, R. Bernards, and T.K. Sixma Crystal structure of murine/human Ubc9 provides insight into the variability of the ubiquitin-conjugating system J. Biol. Chem. 272 34 1997 21381 21387
28. J.J. Lichty, J.L. Malecki, H.D. Agnew, D.J. Michelson-Horowitz, and S. Tan Comparison of affinity tags for protein purification Protein Expr. Purif. 41 1 2005 98 105
29. P.Y. Wu, M. Hanlon, M. Eddins, C. Tsui, R.S. Rogersm, J.P. Jensen, M.J. Matunis, A.M. Weisman, C.P. Wolberger, and C.M. Pickart A conserved catalytic residue in the ubiquitin-conjugating enzyme family EMBO J. 23 24 2004 4876
30. M.H. Bucher, A.G. Evdokimov, and D.S. Waugh Differential effects of short affinity tags on the crystallization of Pyrococcus furiosus maltodextrin-binding protein Acta Crystallogr. D. Biol. Crystallogra. 58 2002 392 397
31. A.K. Mohanty, and M.C. Wiener Membrane protein expression and production: effects of polyhistidine tag length and position Protein Expr. Purif. 33 2 2004 m311 m325