Degradation of subunits of the Sec61p complex, an integral component of the ER membrane, by the ubiquitin-proteasome pathway

EMBO Journal

Volumn 15, Issue 9, 1996, Pages 2069-2076

  Biederer, Thomas ^a   Volkwein, Corinna ^a   Sommer, Thomas ^a  

^a MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

Author keywords

ER degradation; Membrane proteins; Sec61; Ubiquitin proteasome pathway; Yeast

Indexed keywords

MEMBRANE PROTEIN; PROTEASOME; PROTEIN SUBUNIT; UBIQUITIN; UBIQUITIN CONJUGATING ENZYME;

ARTICLE; CYTOPLASM; ENDOPLASMIC RETICULUM; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN MODIFICATION; PROTEIN TRANSPORT; YEAST;

EID: 0029985369     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1002/j.1460-2075.1996.tb00560.x     Document Type: Article

References (35)

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (1992) Short Protocols in Molecular Biology. 2nd edn. John Wiley and Sons, New York.
2. Bonifacino, J.S. and Lippincott-Schwartz, J. (1991) Degradation of proteins within the endoplasmic reticulum. Curr. Opin. Cell Biol., 3, 592-600.
3. Chen, P., Johnson, P., Sommer, T, Jentsch, J. and Hochstrasser, M (1993) Multiple ubiquitin-conjugating enzymes participate in the in vivo degradation of the yeast MATα2 repressor. Cell, 74, 357-369.
4. Ciechanover, A. (1994) The ubiquitin-proteasome proteolytic pathway. Cell, 79, 13-21.
5. Deshaies, R. and Schekman, R. (1987) A yeast mutant defective at an early stage in import of secretory protein precursors into the endoplasmic reticulum. J. Cell Biol., 105, 633-645.
6. Deveraux, Q., Ustrell, V., Pickart, C. and Rechsteiner, M. (1994) A 26S protease subunit that binds ubiquitin conjugates. J. Biol. Chem., 269, 7059-7061.
7. Esnault, Y., Blondel, M.-O., Deshaies, R., Schekman, R. and Kepes, F (1993) The yeast SSS1 gene is essential for secretory protein translocation and encodes a conserved protein of the endoplasmic reticulum. EMBO J., 12, 4083-4093
8. Esnault, Y., Feldheim, D., Blondel, M.-O , Schekman, R. and Kepes, F (1994) SSS1 encodes a stabilizing component of the Sec61 subcomplex of the yeast protein translocation apparatus. J. Biol. Chem., 269, 27478-27485.
9. Finke, K., Plath, K., Panzner, S., Prehn, S., Rapoport, T.A , Hartmann, E and Sommer, T. (1996) A second trimeric complex containing homologs of the Sec61p-complex functions in protein transport across the ER membrane of S. cerevisiae. EMBO J., 15, 1482-1494.
10. Finley, D., Sadis, S., Monia, B.P., Boucher, P., Ecker, D.J., Crooke, S.T. and Chau, V. (1994) Inhibition of proteolysis and cell cycle progression in a multiubiquitination-deficient yeast mutant. Mol. Cell. Biol., 14, 5501-5509.
11. Gardner, A.M., Aviel, S. and Argon, Y. (1993) Rapid degradation of an unassembled immunoglobulin light chain is mediated by a serine protease and occurs in a pre-golgi compartment. J. Biol. Chem , 268, 25940-25947.
12. Gorlich, D. and Rapoport, T.A. (1993) Protein translocation into proteoliposomes reconstituted from purified components of the endoplasmic reticulum membrane. Cell, 75, 615-630.
13. Görlich, D., Prehn, S., Hartmann, E , Kalies, K.-U and Rapoport T.A. (1992) A mammalian homolog of SEC61p and SECYp is associated with ribosomes and nascent polypeptides during translocation Cell, 71, 489-503.
14. Graham, T.R. and Emr, S.D. (1991) Compartmental organization of Golgi-specific protein modification and vacuolar protein sorting events defined in a yeast sec18 (NSF) mutant. J. Cell Biol., 114, 207-218.
15. Guthrie, C. and Fink, G.R. (1991) Guide to Yeast Genetics and Molecular Biology. Academic Press, San Diego, CA.
16. Hartmann, E., Sommer, T., Prehn, S., Görlich, D., Jentsch, S and Rapoport, T A. (1994) Evolutionary conservation of the components of the protein translocation complex. Nature, 367, 654-657.
17. Hememeyer, W., Kleinschmidt, J.A., Saidowsky, J., Escher, C. and Wolf, D.H. (1991) Proteinase yscE, the yeast proteasome/multicalalytic multifunctional proteinase: mutants unravel its function in stress induced proteolysis and uncover its necessity for cell survival. EMBO J., 10, 555-562.
18. Jensen, T.J., Loo, M.A., Pind, S., Williams, D.B., Goldberg, A.L and Riordan, J R. (1995) Multiple proteolytic systems, including the proteasome, contribute to CFTR processing. Cell, 83, 129-135.
19. Jungmann, J., Reins, H.-A., Schobert, C. and Jentsch, S. (1993) Resistance to cadmium mediated by ubiquitin-dependent proteolysis Nature, 361, 369-371.
20. Kihara, A., Akiyama, Y. and Ito, K. (1995) FtsH is required for proteolytic elimination of uncompleted forms of SecY, an essential protein translocase subunit. Proc. Natl. Acad. Sci. USA, 92, 4532-4536.
21. Klausner, R.D. and Sitia, R. (1990) Protein degradation in the endoplasmic reticulum. Cell, 62, 611-614.
22. Lipp, J. and Dobberstein, B (1986) The membrane-spanning segment of invariant chain (I gamma) contains a potentially cleavable signal sequence. Cell, 46, 1103-1112.
23. Löwe, J., Stock, D., Jap, B., Zwickl, P., Baumeister, W. and Huber, R. (1995) Crystal structure of the 20S proteasome from the archacon T. acidophilum at 3.4 Å resolution. Science, 268, 533-539.
24. Mullins, C., Lu, Y.Q., Campbell, A., Fang, H. and Green, N. (1995) A mutation affecting signal peptidase inhibits degradation of an abnormal membrane protein in Saccharomyces cerevisiae. J. Biol. Chem., 270, 17139-17147
25. Panzner, S., Dreier, L., Hartmann, E., Kostka, S. and Rapoport, T. (1995) Post-translational protein translocation in yeast reconstituted with a purified complex of Sec proteins and Kar2p. Cell, 81, 561-570.
26. Peters, J.-M. (1994) Proteasomes, protein degradation machines of the cell. Trends Biochem. Sci., 19, 377-382.
27. Rose, M.D., Misra, L.M. and Vogel, J.P. (1989) KAR2, a karyogamy gene, is the yeast homolog of the mammalian BiP/GRP78 gene. Cell, 57, 1211-1221.
28. Schmid, S.R. and Spiess, M. (1988) Deletion of the amino-terminal domain of asialoglycoprotein receptor H1 allows cleavage of the internal signal sequence. J. Biol. Chem., 263, 16886-16891
29. Seufert, W. and Jentsch, S. (1992) In vivo function of the proteasome in the ubiquitin pathway. EMBO J., 11, 3077-3080.
30. Sogaard, M., Tani, K., Ye, R.R., Geromanos, S., Tempst, P., Kirchhausen, T , Rothman, J.E. and Söllner, T. (1994) A rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles. Cell, 78, 937-948
31. Sommer, T and Jentsch, S (1993) A protein translocation defect linked to ubiquitin conjugation at the endoplasmic reticulum. Nature, 365, 176-179.
32. Sterling, C.J., Rothblatt, J., Hosobuchi, M. Deshaies, R. and Schekman, R. (1992) Protein translocation mutants defective in the insertion of integral membrane proteins into the endoplasmic reticulum. Mol. Biol Cell, 3, 129-142.
33. Taura, T., Baba, T., Akiyama, Y. and Ito, K. (1993) Determinants of the quantity of the stable SecY complex in Escherichia coli. Cell. J. Bac., 175, 7771-7775.
34. Ward, C L , Omura, S. and Kopito, R.R (1995) Degradation of CFTR by the ubiquitin-proteasome pathway. Cell, 83, 121-127.
35. Yuk, M.H. and Lodish, H.F. (1993) Two pathways for the degradation of the H2 subunit of the asialoglycoprotein receptor in the endoplasmic reticulum J. Cell Biol , 123, 1735-1749.