Pseudomonas aeruginosa C5-mannuronan epimerase: Steady-state kinetics and characterization of the product

Biochemistry

Volumn 45, Issue 2, 2006, Pages 552-560

  Jerga, Agoston ^a   Raychaudhuri, Aniruddha ^a   Tipton, Peter A ^a  

^a University of Missouri   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; BIOFILMS; BIOSYNTHESIS; CATALYSIS; CHEMICAL REACTIONS; SPECTROSCOPIC ANALYSIS; VELOCITY;

C5-MANNURONAN EPIMERASES; GULURONATE COMPOSITION; POLYMERIC SUBSTRATE POLYMANNURONAN; PSEUDOMONAS AERUGINOSA C5-MANNURONAN EPIMERASE;

ENZYME KINETICS;

ALGINIC ACID; EPIMERASE; MANNURONAN EPIMERASE; POLYMER; UNCLASSIFIED DRUG;

ARTICLE; BIOFILM; CATALYSIS; ENZYME ACTIVE SITE; ENZYME PURIFICATION; ENZYME SUBSTRATE; FRACTIONATION; NONHUMAN; PH MEASUREMENT; POLYMERIZATION; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; PROTON TRANSPORT; PSEUDOMONAS AERUGINOSA; REACTION ANALYSIS;

ALGINATES; CALCIUM; CARBOHYDRATE EPIMERASES; GLUCURONIC ACID; HEXURONIC ACIDS; HYDROGEN-ION CONCENTRATION; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; METALS; PSEUDOMONAS AERUGINOSA; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); PSEUDOMONAS AERUGINOSA;

EID: 30744475705     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi051862l     Document Type: Article

References (28)

1. Govan, J. R. W., and Harris, G. S. (1986) Pseudomonas aeruginosa and cystic fibrosis: Unusual bacterial adaptation and pathogenesis, Microbiol. Sci. 3, 302-308.
2. Frederiksen, B., Koch, C., and Hoiby, N. (1997) Antibiotic treatment of initial colonization with Pseudomonas aeruginosa postpones chronic infection and prevents deterioration of pulmonary function in cystic fibrosis, Pediatr. Pulmonol. 23, 330-335.
3. Evans, L. R., and Linker, A. (1973) Production and characterization of the slime polysaccharide of Pseudomonas aeruginosa, J. Bacteriol. 116, 915-924.
4. Smidsrod, O., and Draget, K. (1996) Chemistry and physical properties of alginates, Carbohydr. Eur. 14, 29-36.
5. Chitnis, C. E., and Ohman, D. E. (1993) Genetic analysis of the alginate biosynthetic gene cluster of Pseudomonas aeruginosa shows evidence of an operonic structure, Mol. Microbiol. 8, 583-590.
6. Saxena, I. M., Brown, R. M., Fevre, M., and Geremia, R. A. (1995) Multidomain architecture of β-glycosyl transferases: Implications for mechanism of action, J. Bacteriol. 177, 1419-1424.
7. Franklin, M. J., Chitnis, C. E., Gacesa, P., Sonesson, A., White, D. C., and Ohman, D. E. (1994) Pseudomonas aeruginosa AlgG is a polymer level alginate C5-mannuronan epimerase, J. Bacteriol. 176, 1821-1830.
8. Davidson, J. W., Lawson, C. J., and Sutherland, I. W. (1977) Localization of O-acetyl groups in bacterial alginate, J. Gen. Microbiol. 98, 603-606.
9. Rehm, B. H., Boheim, G., Tommassen, J., and Winkler, U. K. (1994) Overexpression of algE in Escherichia coli: Subcellular localization, purification, and ion channel properties, J. Bacteriol. 176, 5639-5647.
10. Sherbrock-Cox, V., Russell, N. J., and Gacesa, P. (1984) The purification and chemical characterization of the alginate present in extracellular material produced by mucoid strains of Pseudomonas aeruginosa, Carbohydr. Res. 135, 147-154.
11. Ertesvag, H., Doseth, B., Larsen, B., Skjak-Braek, G., and Valla, S. (1994) Cloning and expression of an Azotobacter vinelandii C-5-epimerase gene, J. Bacteriol. 176, 2846-2853.
12. Svanem, B. I. G., Skjak-Braek, G., Ertesvag, H., and Valla, S. (1999) Cloning and expression of three new Azotobacter vinelandii genes closely related to a previously described gene family encoding mannuronan C-5-epimerases, J. Bacteriol. 181, 68-77.
13. Ertesvag, H., Hoidal, H. K., Hals, I. K., Rian, A., Doseth, B., and Valla, S. (1995) A family of modular type mannuronan C5-epimerae genes controls alginate structure in Azotobacter vinelandii, Mol. Microbiol. 16, 719-731.
14. Douthit, S. A., Dlakic, M., Ohman, D. E., and Franklin, M. J. (2005) Epimerase active domain of Pseudomonas aeruginosa AlgG, a protein that contains a right-handed β-helix, J. Bacteriol. 187, 4573-4583.
15. Raychaudhuri, A., and Tipton, P. A. (2004) Protocol for amplification of GC-rich sequences from Pseudomonas aeruginosa, BioTechniques 37, 752-756.
16. Iwamoto, Y., Araki, R., Iriyama, K., Oda, T., Fukuda, H., Hayashida, S., and Muramatsu, T. (2001) Purification and characterization of bifunctional alginate lyase from Alteromonas sp. strain No. 272 and its action on saturated oligomeric substrates, Biosci., Biotechnol., Biochem. 65, 133-142.
17. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olsen, B. J., and Klenk, D. C. (1985) Measurement of protein using bicinchoninic acid, Anal. Biochem. 150, 76-85.
18. Steginsky, C. A., Beale, J. M., Floss, H. G., and Mayer, R. M. (1992) Structural determination of alginic acid and the effects of calcium binding as determined by high-field NMR, Carbohydr. Res. 225, 11-26.
19. Larsen, B., Salem, D. M. S. A., Sallam, M. A. E., Mishrikey, M. M., and Beltagy, A. I. (2003) Characterization of the alginates from algae harvested at the Egyptian Red Sea coast, Carbohydr. Res. 338, 2325-2336.
20. Valla, S., Li, J.-P., Ertesvag, H., Barbeyron, T., and Lindahl, U. (2001) Hexuronyl C5-epimerases in alginate and glycosaminoglycan biosynthesis, Biochemie 83, 819-830.
21. Poyner, R. R., Cleland, W. W., and Reed, G. H. (2001) Role of metal ions in catalysis by enolase: An ordered kinetic mechanism for a single substrate enzyme, Biochemistry 40, 8009-8017.
22. 13C NMR spectroscopic studies, Biopolymers 33, 703-711.
23. Hsiu, J., Fischer, E. H., and Stein, E. A. (1964) α-Amylases as calcium-metalloenzymes. II. Calcium and the catalytic activity, Biochemistry 3, 61-66.
24. Liu, D., Shriver, Z., Godavarti, R., Venkataraman, G., and Sasisekharan, R. (1999) The calcium-binding sites of heparinase I from Flavobacterium heparinum are essential for enzymic activity, J. Biol Chem. 274, 4089-4095.
25. Hartmann, M., Holm, O. B., Johansen, G. A. B., Skjak-Braek, G., and Stokke, B. T. (2002) Mode of action of recombinant Azotobacter vinelandii mannuronan C-5 epimerases AlgE2 and AlgE4, Biopolymers, 63, 77-88.
26. Cleland, W. W. (1982) The use of pH studies to determine chemical mechanisms of enzyme-catalyzed reactions, Methods Enzymol. 87, 390-405.
27. Schurks, N., Wingender, J., Flemming, H.-C., and Mayer, C. (2002) Monomer composition and sequence of alginates from Pseudomonas aeruginosa, Int. J. Biol. Macromol. 30, 105-111.
28. Skjak-Braek, G., Grasdalen, H., and Larsen, B. (1986) Monomer sequence and acetylation pattern in some bacterial alginates, Carbohydr. Res. 154, 239-250.