Protocol for amplification of GC-rich sequences from Pseudomonas aeruginosa

BioTechniques

Volumn 37, Issue 5, 2004, Pages 752-756

  Raychaudhuri, Aniruddha ^a,b   Tipton, Peter A ^a  

^a UNIVERSITY OF MISSOURI   (United States)

^b University of Missouri   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; GENOMIC DNA;

ARTICLE; BACTERIAL GENE; DNA BASE COMPOSITION; GENE AMPLIFICATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PSEUDOMONAS AERUGINOSA;

BACTERIA (MICROORGANISMS); PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 8444245605     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/04375bm08     Document Type: Article

References (5)

1. Wilson, R. and R.B. Dowling. 1998. Lung infections 3. Pseudomonas aeruginosa and other related species. Thorax 53:213-219.
2. Stover, C.K., X.Q. Pham, A.L. Erwin, S.D. Mizoguchi, P. Warrener, M.J. Hickey, F.S. Brinkman, W.O. Hufnagle, et al. 2000. Complete genome sequence of Pseudomonas aeruginosa PA01. an opportunistic pathogen. Nature 406:959-964.
3. Goldberg, J.B. and D.E. Ohman. 1984. Cloning and expression in Pseudomonas aeruginosa of a gene involved in the production of alginate. J. Bacteriol. 158:1115-1121.
4. Chen, W. and T. Kuo. 1993. A simple and rapid method for the preparation of gram negative bacterial genomic DNA. Nucleic Acids Res. 21:2260.
5. Kureishi, A. and L.E. Bryan. 1992. Pre-boiling high GC content, mixed primers with 3′ complementation allows the successful PCR amplification of Pseudomonas aeruginosa DNA. Nucleic Acids Res. 20:1155.