DNA spools under tension

Physical Review Letters

Volumn 92, Issue 22, 2004, Pages

  Kulic I M ^a   Schiessel, H ^a  

^a MAX PLANCK INSTITUTE FOR POLYMER RESEARCH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ADSORPTION; CONTINUUM MECHANICS; DEGREES OF FREEDOM (MECHANICS); DNA; ELASTICITY; ELECTROSTATICS; GENES; HYDROGEN; MATHEMATICAL MODELS; POLYMERS; PROTEINS; RNA; STIFFNESS; STRUCTURE (COMPOSITION);

ADSORPTION ENERGY; BENDING ENERGY; ENERGY DENSITY; GENE REGULATION;

MOLECULAR DYNAMICS;

EID: 3042834082     PISSN: 00319007     EISSN: None     Source Type: Journal    
DOI: 10.1103/PhysRevLett.92.228101     Document Type: Article

References (30)

1. Reviewed in R. M. Saecker and M. T. Record, Curr. Opin. Struct. Biol. 12, 311 (2002).
2. O. V. Tsodikov et al., J. Mol. Biol. 294, 639 (1999).
3. S. C. Kampranis, A. d. Bates, and A. Maxwell, Proc. Natl. Acad. Sci. U.S.A. 96, 8414 (1999).
4. C. Rivetti, M. Guthold, and C. Bustamante. EMBO J. 18, 4464 (1999).
5. K. Luger, A. W. Mäder, R. K. Richmond, D. F. Sargent, and T. J. Richmond, Nature (London) 389, 251 (1997).
6. K. B. Thurmond, E. E. Remsen, T. Kowalewski, and K. L. Wooley, Nucleic Acids Res. 27, 2966 (1999).
7. Reviewed in V. A. Bloomfield, Curr Opin Struct Biol 6. 334 (1996); Biopolymers 44, 269 (1997).
8. Reviewed in V. A. Bloomfield, Curr Opin Struct Biol 6. 334 (1996); Biopolymers 44, 269 (1997).
9. T. R. Strick et al. Rep. Prog. Phys. 66, 1 (2003).
10. M. C. Williams et al., Proc. Natl. Acad. Sci. U.S.A. 98. 6121 (2001); M. Hegner, S. B. Smith, and C. Bustamante, Proc. Natl. Acad. Sci. U.S.A. 96, 10109 (1999).
11. M. C. Williams et al., Proc. Natl. Acad. Sci. U.S.A. 98. 6121 (2001); M. Hegner, S. B. Smith, and C. Bustamante, Proc. Natl. Acad. Sci. U.S.A. 96, 10109 (1999).
12. C. G. Baumann et al., Biophys. J. 78, 1965 (2000).
13. Y. Murayama, Y. Sakamaki, and M. Sano, Phys. Rev. Lett 90, 018102 (2003).
14. K. Svoboda and S. M. Block, Cell 77, 773 (1994); H. Yin et al., Science 270, 1653 (1995).
15. K. Svoboda and S. M. Block, Cell 77, 773 (1994); H. Yin et al., Science 270, 1653 (1995).
16. E. Evans and K. Ritchie, Biophys. J. 72, 1541 (1997); E. Evans, Biophys. Chem. 82, 83 (1999).
17. E. Evans and K. Ritchie, Biophys. J. 72, 1541 (1997); E. Evans, Biophys. Chem. 82, 83 (1999).
18. B. D. Brower-Toland et al., Proc. Natl. Acad. Sci. U.S.A. 99, 1960 (2002).
19. Y. Cui and C. Bustamante, Proc. Natl. Acad. Sci. U.S.A. 97, 127 (2002).
20. R. Bruinsma and J. Rudnick, Biophys. J. 76, 1725 (1999).
21. G. C. Cecchi and M. O. Magnasco, Phys. Rev. Lett. 76, 1968 (1996); D. Bartolo, I. Derényi, and A. Ajdari, Phys. Rev. E 65, 051910 (2002).
22. G. C. Cecchi and M. O. Magnasco, Phys. Rev. Lett. 76, 1968 (1996); D. Bartolo, I. Derényi, and A. Ajdari, Phys. Rev. E 65, 051910 (2002).
23. K. J. Polach and J. Widom, J. Mol. Biol. 254, 130 (1995); 258, 800 (1996).
24. K. J. Polach and J. Widom, J. Mol. Biol. 254, 130 (1995); 258, 800 (1996).
25. The experiments were performed on DNA chains with up to 17 nucleosomes complexed at well-defined positions. In the force range of interest their coupling can be safely neglected since the intranucleosomal distance d ∼ 40 nm exceeds the DNA-linker induced tension length A [16].
26. J. F. Marko and E. D. Siggia, Biophys. J. 73, 2173 (1997); K.-K. Kunze and R. R. Netz, Phys. Rev. E 66, 011918 (2002)
27. J. F. Marko and E. D. Siggia, Biophys. J. 73, 2173 (1997); K.-K. Kunze and R. R. Netz, Phys. Rev. E 66, 011918 (2002)
28. F. In this case all degrees of freedom of the chain are in quasiequilibrium, except for the slowest process, the spool unwrapping; for a general discussion of this issue for semiflexible chains, cf. E. Evans and K. Ritchie, Biophys. J. 76, 2439 (1999).
29. -1 of that frequency.
30. el, that is on the order of 8 to 10 pN - still much smaller than the measured unwrapping forces.