Fusion of Aequorea victoria GFP and aequorin provides their Ca 2+-induced interaction that results in red shift of GFP absorption and efficient bioluminescence energy transfer

Biochemical and Biophysical Research Communications

Volumn 320, Issue 3, 2004, Pages 703-711

  Gorokhovatsky, Andrey Yu ^a   Marchenkov, Victor V ^b   Rudenko, Natalia V ^a   Ivashina, Tanya V ^b,c   Ksenzenko, Vladimir N ^b   Burkhardt, Nils ^d   Semisotnov, Gennady V ^b   Vinokurov, Leonid M ^a   Alakhov, Yuli B ^a  

^a SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

^b INSTITUTE OF PROTEIN RESEARCH   (Russian Federation)

^c SKRYABIN INSTITUTE OF BIOCHEMISTRY AND PHYSIOLOGY OF MICROORGANISMS   (Russian Federation)

^d BAYER AG   (Germany)

Author keywords

Aequorin; Bioluminescence energy transfer; Fusion proteins; Green fluorescent protein; Obelin; Protein protein interaction

Indexed keywords

AEQUORIN; CALCIUM ION; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; OBELIN; PHOTOPROTEIN; UNCLASSIFIED DRUG;

AEQUOREA VICTORIA; ARTICLE; BIOLUMINESCENCE; BIOLUMINESCENCE RESONANCE ENERGY TRANSFER; BLUE LIGHT; CALCIUM SIGNALING; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION; GENE EXPRESSION SYSTEM; IN VITRO STUDY; JELLYFISH; MOLECULAR INTERACTION; NONHUMAN; OBELIA LONGISSIMA; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION;

AEQUORIN; ANIMALS; CALCIUM; ENERGY TRANSFER; HYDROZOA; KINETICS; LUMINESCENT MEASUREMENTS; LUMINESCENT PROTEINS; RECOMBINANT FUSION PROTEINS; SCYPHOZOA;

AEQUOREA; AEQUOREA VICTORIA; ESCHERICHIA COLI; OBELIA; OBELIA LONGISSIMA; SCYPHOZOA; VICTORIA;

EID: 3042727962     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2004.06.014     Document Type: Article

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