Activity and expression of progesterone metabolizing 5α-reductase, 20α-hydroxysteroid oxidoreductase and 3α9(β -hydroxysteroid oxidoreductase in tumorigenic (MCF-7, MDA-MB-231, T-47D) and nontumorigenic (MCF-10A) human breast cancer cells

BMC Cancer

Volumn 3, Issue , 2003, Pages

  Wiebe, John P ^a   Lewis, Michael J ^a  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

20ALPHA HYDROXYSTEROID DEHYDROGENASE; 3ALPHA HYDROXYSTEROID DEHYDROGENASE; 5ALPHA PREGNANE DERIVATIVE; CARBON 14; PROGESTERONE; STEROID 5ALPHA REDUCTASE; TRITIUM; 3 ALPHA HYDROXYSTEROID DEHYDROGENASE (B SPECIFIC); 3(OR 17)BETA HYDROXYSTEROID DEHYDROGENASE; CHOLESTENONE 5ALPHA REDUCTASE;

ARTICLE; BREAST CANCER; CANCER CELL CULTURE; CELL TYPE; CONTROLLED STUDY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; INCUBATION TIME; PROGESTERONE METABOLISM; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STATISTICAL ANALYSIS; BREAST TUMOR; ENZYMOLOGY; FEMALE; METABOLISM; TUMOR CELL LINE;

20-ALPHA-HYDROXYSTEROID DEHYDROGENASE; 3-ALPHA-HYDROXYSTEROID DEHYDROGENASE (B-SPECIFIC); 3-HYDROXYSTEROID DEHYDROGENASES; BREAST NEOPLASMS; CELL LINE, TUMOR; CHOLESTENONE 5 ALPHA-REDUCTASE; FEMALE; HUMANS; PROGESTERONE;

EID: 3042716504     PISSN: 14712407     EISSN: None     Source Type: Journal    
DOI: 10.1186/1471-2407-3-9     Document Type: Article

References (49)

1. Wiebe JP, Muzia D, Hu J, Szwajcer D, Hill SA and Seachrist JL The 4-pregnene and 5α-pregnane progesterone metabolites formed in nontumorous and tumorous breast tissue have opposite effects on breast cell proliferation and adhesion Cancer Res 2000, 60:936-943
2. Wiebe JP and Muzia D The endogenous progesterone metabolite, 5α-pregnane-3,20-dione, decreases cell-substrate attachment, adhesion plaques, vinculin expression, and polymerized F-actin in MCF-7 breast cancer cells Endocrine 2001, 16:7-14
3. Weiler PJ and Wiebe JP Plasma membrane receptors for the cancer-regulating progesterone metabolites, 5α-pregnane-3,20-dione and 3α-hydroxy-4-pregnen-20- one in MCF-7 breast cancer cells Biochem Biophys Res Commun 2000, 272:731-737
4. Soule HD, Maloney TM, Wolman SR, Peterson WD Jr, Brenz R, Mc-Grath CM, Russo J, Pauley RJ, Jones RF and Brooks SC Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10 Cancer Res 1990, 50:6075-6086
5. Anderson WA, Perotti ME, McManaway M, Lindsey S and Eckberg WR Similarities and differences in the ultrastructure of two hormone-dependent and one independent human breast carcinoma grown in athymic nude mice: comparison with the rat DMBA-induced tumor and normal secretory mammocytes J Submicrosc Cytol 1984, 16:673-690
6. Soto AM, Murai JT, Siiteri PK and Sonnenschein C Control of cell proliferation: evidence for negative control on estrogen-sensitive T47D human breast cancer cells Cancer Res 1986, 46:2271-2275
7. Horwitz KB, Costlow ME and McGuire WL MCF-7; a human breast cancer cell line with estrogen, androgen, progesterone, and glucocorticoid receptors Steroids 1975, 26:785-795
8. Read LD, Snider CE, Miller JS, Greene GL and Katzenellenbogen BS Ligand-modulated regulation of progesterone receptor messenger ribonucleic acid and protein in human breast cancer cell lines Mol Endocrinol 1988, 2:263-271
9. Wiebe JP, Barr KJ and Buckingham KD A radioimmunoassay for the regulatory allylic steroid, 3α-hydroxy-4-pregnen-20-one (3αHP) J Steroid Biochem Mol Biol 1991, 38:505-512
10. Wiebe JP, Deline C, Buckingham KD, Dave V and Stothers JB Synthesis of the allylic gonadal steroids, 3α -hydroxy-4-pregnen-20-one and 3α-hydroxy-4-androsten-17-one, and of 3α-hydroxy-5 alpha-pregnan-20-one Steroids 1985, 45:39-51
11. Wiebe JP, Dave V and Stothers JB Synthesis and characteristics of allylic 4-pregnene-3,20-diols found in gonadal and breast tissues Steroids 1986, 47:249-259
12. Glasier MA, Wiebe JP and Hobkirk R Progesterone metabolism by guinea pig intrauterine tissues J Steroid Biochem Mol Biol 1994, 51:199-207
13. Wiebe JP, Boushy D and Wolfe M Synthesis, metabolism and levels of the neuroactive steroid, 3α-hydroxy-4-pregnen-20-one (3αHP), in rat pituitaries Brain Res 1997, 764:158-166
14. Bradford MM A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 1976, 72:248-254
15. Chen W, Zouboulis CC, Fritsch M, Blume-Peytavi U, Kodelja V, Goerdt S, Luu-The V and Orfanos CE Evidence of heterogeneity and quantitative differences of the type 1 5α-reductase expression in cultured human skin cells - evidence of its presence in melanocytes J Invest Dermatol 1998, 110:84-89
16. Boehmer AL, Brinkmann AO, Nijman RM, Verleun-Mooijman MC, de Ruiter P, Niermeijer MF and Drop SL Phenotypic variation in a family with partial androgen insensitivity syndrome explained by differences in 5α-dihydrotestosterone availability J Clin Endocrinol Metab 2001, 86:1240-1246
17. Schmittgen TD and Zakrajsek BA Effect of experimental treatment on housekeeping gene expression: validation by realtime, quantitative RT-PCR J Biochem Biophys Methods 2000, 46:69-81
18. Mori M and Tamaoki BI In vitro metabolism of progesterone in the mammary tumor and the normal mammary gland of GRS/A strain of mice and dependency of some steroid-metabolizing enzyme activities upon ovarian function Eur J Cancer 1980, 16:185-193
19. Mori M, Tominaga T and Tamaoki BI Steroid metabolism in normal mammary gland and in the dimethylbenzanthracene-induced mammary tumor of rats Endocrinology 1978, 102:1387-1397
20. Mori M, Tominaga T, Kitamura M, Saito T and Tamaoki B Effect of oophorectomy on progesterone metabolism in DMBA-induced mammary tumours of the rat Eur J Cancer 1980, 16:1373-1375
21. Eechaute W, de Thibault de Boesinghe L and Lacroix E Steroid metabolism and steroid receptors in dimethylbenz(a)anthracene-induced rat mammary tumors Cancer Res 1983, 43:4260-4265
22. Lloyd RV Studies on the progesterone receptor content and steroid metabolism in normal and pathological human breast tissues J Clin Endocrinol Metab 1979, 48:585-593
23. Verma U, Kapur MM and Laumas KR Characterization of progesterone receptors and metabolism of progesterone in the normal and cancerous human mammary gland J Steroid Biochem 1978, 9:569-577
24. Russell DW and Wilson JD Steroid 5α-reductase: two genes/two enzymes Annu Rev Biochem 1994, 63:25-61
25. Andersson S and Russell DW Structural and biochemical properties of cloned and expressed human and rat steroid 5α -reductases Proc Natl Acad Sci U S A 1990, 87:3640-3644
26. Andersson S, Berman DM, Jenkins EP and Russell DW Deletion of steroid 5α-reductase 2 gene in male pseudohermaphroditism Nature 1991, 354:159-161
27. Russell DW, Berman DM, Bryant JT, Cala KM, Davis DL, Landrum CP, Prihoda JS, Silver RI, Thigpen AE and Wigley WC The molecular genetics of steroid 5α-reductases Recent Prog Horm Res 1994, 49:275-284
28. Thigpen AE, Silver RI, Guileyardo JM, Casey ML, McConnell JD and Russell DW Tissue distribution and ontogeny of steroid 5α -reductase isozyme expression J Clin Invest 1993, 92:903-910
29. Suzuki T, Darnel AD, Akahira JI, Ariga N, Ogawa S, Kaneko C, Takeyama J, Moriya T and Sasano H 5α-reductases in human breast carcinoma: possible modulator of in situ androgenic actions J Clin Endocrinol Metab 2001, 86:2250-2257
30. Spurdle AB, Hopper JL, Chen X, Dite GS, McCredie MR, Giles GG, Venter DJ, Southey MC, Purdie DM and Chenevix-Trench G The steroid 5α-reductase type II TA repeat polymorphism is not associated with risk of breast or ovarian cancer in Australian women Cancer Epidemiol Biomarkers Prev 2001, 10:1287-1293
31. Scorilas A, Bharaj B, Giai M and Diamandis EP Codon 89 polymorphism in the human 5α-reductase gene in primary breast cancer Br J Cancer 2001, 84:760-767
32. Bharaj B, Scorilas A, Giai M and Diamandis EP TA repeat polymorphism of the 5α-reductase gene and breast cancer Cancer Epidemiol Biomarkers Prev 2000, 9:387-393
33. Normington K and Russell DW Tissue distribution and kinetic characteristics of rat steroid 5α-reductase isozymes. Evidence for distinct physiological functions J Biol Chem 1992, 267:19548-19554
34. Poletti A, Rabuffetti M and Martini L Effect of suramin on the biological activity of the two isoforms of the rat 5α-reductase Steroids 1996, 61:504-505
35. Thigpen AE, Cala KM and Russell DW Characterization of Chinese hamster ovary cell lines expressing human steroid 5α -reductase isozymes J Biol Chem 1993, 268:17404-17412
36. Wilson JD The pathogenesis of benign prostatic hyperplasia Am J Med 1980, 68:745-756
37. Bruchovsky N, Rennie PS, Batzold FH, Goldenberg SL, Fletcher T and McLoughlin MG Kinetic parameters of 5α-reductase activity in stroma and epithelium of normal, hyperplastic, and carcinomatous human prostates J Clin Endocrinol Metab 1988, 67:806-816
38. Iehle C, Delos S, Guirou O, Tate R, Raynaud JP and Martin PM Human prostatic steroid 5α-reductase isoforms - a comparative study of selective inhibitors J Steroid Biochem Mol Biol 1995, 54:273-279
39. Bruchovsky N, Sadar MD, Akakura K, Goldenberg SL, Matsuoka K and Rennie PS Characterization of 5α-reductase gene expression in stroma and epithelium of human prostate J Steroid Biochem Mol Biol 1996, 59:397-404
40. Pollow K, Lubbert H, Boquoi E and Pollow B Progesterone metabolism in normal human endometrium during the menstrual cycle and in endometrial carcinoma J Clin Endocrinol Metab 1975, 41:729-737
41. Zhang Y, Dufort I, Rheault P and Luu-The V Characterization of a human 20α-hydroxysteroid dehydrogenase J Mol Endocrinol 2000, 25:221-228
42. Dufort I, Soucy P, Labrie F and Luu-The V Molecular cloning of human type 3 3α-hydroxysteroid dehydrogenase that differs from 20α-hydroxysteroid dehydrogenase by seven amino acids Biochem Biophys Res Commun 1996, 228:474-479
43. Penning TM, Burczynski ME, Jez JM, Hung CF, Lin HK, Ma H, Moore M, Palackal N and Ratnam K Human 3α-hydroxysteroid dehydrogenase isoforms (AKR1C1-AKR1C4) of the aldo-keto reductase superfamily: functional plasticity and tissue distribution reveals roles in the inactivation and formation of male and female sex hormones Biochem J 2000, 351:67-77
44. Jez JM, Bennett MJ, Schlegel BP, Lewis M and Penning TM Comparative anatomy of the aldo-keto reductase superfamily Biochem J 1997, 326:625-636
45. Dufort I, Labrie F and Luu-The V Human types 1 and 3 3α-hydroxysteroid dehydrogenases: differential lability and tissue distribution J Clin Endocrinol Metab 2001, 86:841-846
46. Raju U, Sklarew RJ, Post J and Levitz M Steroid metabolism in human breast cancer cell lines Steroids 1978, 32:669-680
47. Fennessey PV, Pike AW, Gonzalez-Aller C and Horwitz KB Progesterone metabolism in T47Dco human breast cancer cells - I. 5α-pregnan-3β,6α-diol-20-one is the secreted product J Steroid Biochem 1986, 25:641-648
48. Theriault C and Labrie F Multiple steroid metabolic pathways in ZR-75-1 human breast cancer cells J Steroid Biochem Mol Biol 1991, 38:155-164
49. Henderson BE and Feigelson HS Hormonal carcinogenesis Carcinogenesis 200, 21:427-433