Functional role of RhoA in growth regulation of primary hepatocytes

Journal of Biochemistry

Volumn 135, Issue 5, 2004, Pages 631-637

  Dohda, Takeaki ^a   Nakamura, Yuka ^a   Kamihira, Masamichi ^a   Iijima, Shinji ^a  

^a NAGOYA UNIVERSITY   (Japan)

Author keywords

Cadherin; HGF; Monolayer; Primary hepatocyte; Rho; Spheroid

Indexed keywords

BACTERIAL ENZYME; CADHERIN; CALCIUM; CELL PROTEIN; GUANOSINE TRIPHOSPHATASE; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; SCATTER FACTOR;

ADOLESCENT; ANIMAL CELL; ARTICLE; CALCIUM DEPLETION; CELL ADHESION; CELL GROWTH; CELL LEVEL; CLOSTRIDIUM BOTULINUM; CULTURE MEDIUM; ENZYME ACTIVATION; GROWTH REGULATION; LIVER CELL; LIVER CELL CULTURE; MALE; MONOLAYER CULTURE; NONHUMAN; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN SYNTHESIS; RAT;

ADP RIBOSE TRANSFERASES; ANIMALS; BOTULINUM TOXINS; CADHERINS; CALCIUM; CELL PROLIFERATION; CELLS, CULTURED; GENES, DOMINANT; GLUTATHIONE TRANSFERASE; GUANOSINE TRIPHOSPHATE; HEPATOCYTE GROWTH FACTOR; HEPATOCYTES; MALE; RATS; RATS, SPRAGUE-DAWLEY; RECOMBINANT FUSION PROTEINS; RHOA GTP-BINDING PROTEIN; TIME FACTORS;

ANIMALIA; BACTERIA (MICROORGANISMS); CLOSTRIDIUM; CLOSTRIDIUM BOTULINUM;

EID: 3042716239     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvh076     Document Type: Article

References (28)

1. Chen, S.C., Mullon, C., Kahaku, E., Watanabe, F., Hewitt, W., Eguchi, S., Middleton, Y., Arkadopoulos, N., Rozga, J., Solomon, B., and Demetriou, A.A. (1997) Treatment of severe liver failure with a bioartificial liver. Ann. N. Y. Acad. Sci. 831, 350-360
2. Koide, N., Sakaguchi, K., Koide, Y., Asano, K., Kawaguchi, M., Matsushima, H., Takenami, T., Shinji, T., Mori, M., and Tsuji, T. (1990) Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environments. Exp. Cell Res. 186, 227-235
3. Matsushita, T., Ijima, H., Koide, N., and Funatsu, K. (1991) High albumin production by multicellular spheroids of adult rat hepatocytes formed in the pores of polyurethane foam. Appl. Microbiol. Biotechnol. 36, 324-326
4. Tobe, S., Takei, Y., Kobayashi, K., and Akaike, T. (1992) Receptor-mediated formation of multilayer aggregates of primary cultured adult rat hepatocytes on lactose-substituted polystyrene. Biochem. Biophys. Res. Commun. 184, 225-230
5. Hamamoto, R., Yamada, K., Kamihira, M., and Iijima, S. (1998) Differentiation and proliferation of primary rat hepatocytes cultured as spheroids. J. Biochem. 124, 972-979
6. Yamada, K., Kamihira, M., Hamamoto, R., and Iijima, S. (1998) Efficient induction of hepatocyte spheroids in a suspension culture using a water-soluble synthetic polymer as an artificial matrix. J. Biochem. 123, 1017-1023
7. Yamada, K., Kamihira, M., and Iijima, S. (1999) Enhanced cell aggregation and liver functions using polymers modified with a cell-specific ligand in primary hepatocyte cultures. J. Biosci. Bioeng. 88, 557-562
8. Yamada, K., Kamihira, M., and Iijima, S. (2001) Self-organization of liver constitutive cells mediated by artificial matrix and improvement of liver functions in long-term culture. Biochem. Eng. J. 8, 135-143
9. Wu, F.J., Friend, J.R., Lazar, A., Mann, H.J., Remmel, R.P., Cerra, F.B., and Hu, W.S. (1996) Hollow fiber bioartificial liver utilizing collagen-entrapped porcine hepatocyte spheroids. Biotechnol. Bioeng. 52, 34-44
10. Fausto, N. (1991) Growth factors in liver development, regeneration, and carcinogenesis. Prog. Growth Factor Res. 3, 219-234
11. Hamamoto, R., Seko, H., Kamimura, R., Yamada, K., Murai, K., Kamihira, M., and Iijima, S. (1999) Growth induction of rat primary hepatocytes using antisense oligonucleotides. J. Biosci. Bioeng. 88, 310-315
12. Hamamoto, R., Kamihira, M., and Iijima, S. (1999) Intermittent addition of HGF and TGF-β1 in rat primary hepatocyte culture. Cytotechnology 31, 111-120
13. Fukata, M. and Kaibuchi, K. (2001) Rho-family GTPases in cadherin-mediated cell-cell adhesion. Nat. Rev. 2, 887-897
14. Etienne-Manneville, S. and Hall, A. (2002) Rho-GTPases in cell biology. Nature 420, 629-635
15. Burnette, W.N. (1981) "Western blotting": electrophoreic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 112, 195-203
16. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
17. Ren, X.D. and Schwartz, M.A. (2000) Determination of GTP loading on Rho. Methods Enzymol. 325, 264-272
18. Yoshioka, K., Matsumura, F., Akedo, H., and Itoh, K. (1998) Small GTP-binding protein Rho stimulates the actomyosin system, leading to invasion of tumor cells. J. Biol. Chem. 273, 5146-5154
19. Arthur, W.T., Noren, N.K., and Burridge, K. (2002) Regulation of Rho family GTPase by cell-cell and cell-matrix adhesion. Biol. Res. 35, 239-246
20. Noren, N.K., Niessen, C.M., Gumbiner, B.M., and Burridge, K. (2001) Cadherin engagement regulates Rho family GTPases. J. Biol. Chem. 276, 33305-33308
21. Kim, T.H., Bowen, W.C., Stolz, D.B., Runge, D., Mars, W.M., and Michalopoulos, G.K. (1998) Differential expression and distribution of focal adhesion and cell adhesion molecules in rat hepatocyte differentiation. Exp. Cell Res. 244, 93-104
22. 2+-independent regulation of the thyroid epithelial junction complex by protein kinases. Exp. Cell Res. 225, 1-11
23. Noren, N.K., Arthur, W.T., and Burridge, K. (2003) Cadherin engagement inhibits RhoA via p190RhoGAP. J. Biol. Chem. 278, 13615-13618
24. Waf1/Cip1. Nature 394, 295-299
25. Sahai, E., Olson, M.F., and Marshall, C.J. (2001) Cross-talk between Ras and Rho signalling pathways in transformation favours proliferation and increased motility. EMBO J. 20, 755-766
26. Kamei, T., Matozaki, T., Sakisaka, T., Kodama, A., Yokoyama, S., Peng, Y.F., Nakano, K., Takaishi, K., and Takai, Y. (1999) Coendocytosis of cadherin and c-Met coupled to disruption of cell-cell adhesion in MDCK cells-regulation by Rho, Rac, and Rab small G proteins. Oncogene 18, 6776-6784
27. Crepaldi, T., Pollack, A.L., Prat, M., Zborek, A., Mostov, K., and Comoglio, P.M. (1994) Targeting of the SF/HGF receptor to the basolateral domain of polarized epithelial cells. J. Cell Biol. 125, 313-320
28. Rubin, E.J., Gill, D.M., Boquet, P., and Popoff, M.R. (1988) Functional modification of a 21-kilodalton G protein when ADP-ribosylated by exoenzyme C3 of Clostridium botulinum. Mol. Cell. Biol. 8, 418-426