Point mutations in transmembrane helices 2 and 3 of ExbB and TolQ affect their activities in Escherichia coli K-12

Journal of Bacteriology

Volumn 186, Issue 13, 2004, Pages 4402-4406

  Braun, Volkmar ^a   Herrmann, Christina ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; EXBB PROTEIN; GLUTAMIC ACID; MEMBRANE PROTEIN; MUTANT PROTEIN; TOLQ PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; CELL MEMBRANE TRANSPORT; GENETIC CONSERVATION; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTON TRANSPORT;

BACTERIAL PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FERRICHROME; POINT MUTATION; PROTEIN STRUCTURE, SECONDARY; STRUCTURE-ACTIVITY RELATIONSHIP;

ESCHERICHIA COLI; ESCHERICHIA COLI K12; NEGIBACTERIA;

EID: 3042615583     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.186.13.4402-4406.2004     Document Type: Article

References (29)

1. Blair, D. F. 2003. Flagellar movement driven by proton translocation. FEBS Lett. 545:86-95.
2. Blair, D. F., and H. C. Berg. 1990. The MotA protein of E. coli is a proton-conducting component of the flagellar motor. Cell 60:439-449.
3. Blair, D. F., and H. C. Berg. 1991. Mutations in the MotA protein of E. coli reveal domains critical for proton conduction. J. Mol. Biol. 221:1433-1442.
4. Bradbeer, C. 1993. The proton motive force drives the outer membrane transport of cobalamin in Escherichia coli. J. Bacteriol. 175:3146-3150.
5. Braun, V. 1989. The structurally related exbB and tolQ genes are interchangeable in conferring tonB-dependent colicin, bacteriophage, and albomycin sensitivity. J. Bacteriol. 171:6387-6390.
6. Braun, V. 1995. Energy-coupled transport and signal transduction through the gram-negative outer membrane via TonB-ExbB-ExbD-dependent receptor proteins. FEMS Microbiol. Rev. 16:295-307.
7. Braun, V., S. Gaisser, C. Herrmann, K. Kampfenkel, H. Killmann, and I. Traub. 1996. Energy-coupled transport across the outer membrane of Escherichia coli: ExbB binds ExbD and TonB in vitro, and leucine 132 in the periplasmic region and aspartate 25 in the transmembrane region are important for ExbD activity. J. Bacteriol. 178:2836-2845.
8. Braun, V., and C. Herrmann. 1993. Evolutionary relationship of uptake systems for biopolymers in Escherichia coli: cross-complementation between the TonB-ExbB-ExbD and the TolA-TolQ-TolR proteins. Mol. Microbiol. 8:261-268.
9. Braun, V., S. I. Patzer, and K. Hantke. 2002. Ton-dependent colicins and microcins: modular design and evolution. Biochimie 84:365-380.
10. Cascales, E., R. Lloubès, and J. N. Sturgis. 2001. The TolQ-TolR proteins energize TolA and share homologies with the flagellar motor proteins MotA-MotB. Mol. Microbiol. 42:795-807.
11. Chang, C., A. Mooser, A. Plückthun, and A. Wlodawer. 2001. Crystal structure of the dimeric C-terminal domain of TonB reveals a novel fold. J. Biol. Chem. 276:27535-27540.
12. Eick-Helmerich, K., and V. Braun. 1989. Import of biopolymers into Escherichia coli: nucleotide sequences of the exbB and exbD genes are homologous to those of the tolQ and tolR genes, respectively. J. Bacteriol. 171:5117-5126.
13. Higgs, P. I., R. A. Larsen, and K. Postle. 2002. Quantification of known components of the Escherichia coli TonB energy transduction system: TonB, ExbB, ExbD and FepA. Mol. Microbiol. 44:271-281.
14. Kampfenkel, K., and V. Braun. 1992. Membrane topology of the Escherichia coli ExbD protein. J. Bacteriol. 174:5485-5487.
15. Kampfenkel, K., and V. Braun. 1993. Topology of the ExbB protein in the cytoplasmic membrane of Escherichia coli. J. Biol. Chem. 268:6050-6057.
16. Kampfenkel, K., and V. Braun. 1993. Membrane topologies of the TolQ and TolR proteins of Escherichia coli: inactivation of TolQ by a missense mutation in the proposed first transmembrane segment. J. Bacteriol. 175:4485-4491.
17. Kojima, S., and D. F. Blair. 2001. Conformational change in the stator of the bacterial flagellar motor. Biochemistry 40:13041-13050.
18. Larsen, R. A., and K. Postle. 2001. Conserved residues Ser(16) and His(20) and their relative positioning are essential for TonB activity, cross-linking of TonB with ExbB, and the ability of TonB to respond to proton motive force. J. Biol. Chem. 276:8111-8117.
19. Larsen, R. A., M. G. Thomas, G. E. Wood, and K. Postle. 1994. Partial suppression of an Escherichia coli TonB transmembrane domain mutation (ΔV17) by a missense mutation in ExbB. Mol. Microbiol. 13:627-640.
20. Lazzaroni, J., J.-F. Dubuisson, and A. Vianney. 2002. The Tol proteins of Escherichia coli and their involvement in the translocation of group A colicins. Biochimie 84:391-397.
21. Müller, M., A. Vianney, J.-C. Lazzaroni, R. E. Webster, and R. Portalier. 1993. Membrane topology of the Escherichia coli TolR protein required for cell envelope integrity. J. Bacteriol. 175:6059-6061.
22. Postle, K., and R. J. Kadner. 2003. Touch and go: tying TonB to transport. Mol. Microbiol. 49:869-882.
23. Prilipov, A., P. S. Phale, P. Van Gelder, J. P. Rosenbusch, and R. Koebnik. 1998. Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins from E. coli. FEMS Microbiol. Lett. 163:65-72.
24. Sauter, A., P. Howard, and V. Braun. 2003. In vivo evidence for TonB dimerization. J. Bacteriol. 185:5747-5754.
25. Schmitt, R. 2003. Helix rotation model of the flagellar rotary motor. Biophys. J. 85:843-852.
26. Traub, I., S. Gaisser, and V. Braun. 1993. Activity domains of the TonB protein. Mol. Microbiol. 8:409-423.
27. Vianney, A., T. M. Lewin, W. F. Beyer., J. Lazzaroni, R. Portalier, and R. E. Webster. 1994. Membrane topology and mutational analysis of the TolQ protein of Escherichia coli required for the uptake of macromolecules and cell envelope integrity. J. Bacteriol. 176:822-829.
28. Wang, R. F., and S. R. Kushner. 1991. Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli. Gene 100:195-199.
29. Zhai, Y. F., W. Heine, and M. H. Saier. 2003. Molecular modelling of the bacterial outer membrane receptor energizer, ExbBD/TonB, based on homology with the flagellar motor, MotAB. Biochim. Biophys. Acta 1614:201-210.