Development of a Western blotting assay to discriminate Brucella spp. and Yersinia enterocolitica O:9 infections in sheep

New Microbiologica

Volumn 27, Issue 2, 2004, Pages 155-161

  Corrente, M ^a   Desario, C ^a   Greco, G ^a   Buonavoglia, D ^b   Pratelli, A ^a   Madio, A ^a   Scaltrito, D ^c   Consenti, B ^c   Buonavoglia, C ^a  

^a UNIVERSITY OF BARI   (Italy)

^b UNIVERSITY OF MESSINA   (Italy)

^c Ist Zooprofilattico Sperim D   (Italy)

Author keywords

Brucella spp.; OMPs; Sheep; Western blotting; Yersinia enterocolitica O:9

Indexed keywords

BACTERIAL PROTEIN; BACTERIAL ANTIGEN; BACTERIUM ANTIBODY; EPITOPE; OUTER MEMBRANE PROTEIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIAL INFECTION; BACTERIAL STRAIN; BACTERIUM DETECTION; BLOOD SAMPLING; BRUCELLA MELITENSIS; BRUCELLOSIS; CONTROLLED STUDY; CORRELATION ANALYSIS; DIAGNOSTIC VALUE; DISCRIMINANT ANALYSIS; EXPERIMENTAL TEST; INTERMETHOD COMPARISON; NONHUMAN; SERODIAGNOSIS; SEROLOGY; SHEEP; WESTERN BLOTTING; YERSINIA ENTEROCOLITICA; ANIMAL; ANIMAL DISEASE; BLOOD; DIFFERENTIAL DIAGNOSIS; IMMUNOLOGY; ISOLATION AND PURIFICATION; METHODOLOGY; SHEEP DISEASE; YERSINIOSIS;

ANIMALIA; BACTERIA (MICROORGANISMS); BRUCELLA; BRUCELLA MELITENSIS; OVIS; OVIS ARIES; YERSINIA; YERSINIA ENTEROCOLITICA;

ANIMALS; ANTIBODIES, BACTERIAL; ANTIGENS, BACTERIAL; BACTERIAL OUTER MEMBRANE PROTEINS; BLOTTING, WESTERN; BRUCELLOSIS; DIAGNOSIS, DIFFERENTIAL; IMMUNODOMINANT EPITOPES; SHEEP; SHEEP DISEASES; YERSINIA ENTEROCOLITICA; YERSINIA INFECTIONS;

EID: 3042576363     PISSN: 11217138     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (22)

1. AHVONEN, P. AND SIEVERS, K. (1969) Yersinia enterocolitica infection associated with Brucella agglutinins. Clinical features of 24 patients. Acta Medica Scandinavica, 185, 121-125.
2. th Edition. Office International des Epizooties, Paris.
3. AKKERMANS, J.P.W.M. AND HILL, W.K.W. (1972) Yersinia enterocolitica serotype 9 infection as a factor interfering with serodiagnosis of Brucella infections in swine. Netherlands Journal of Veterinary Science, 5, 73-80.
4. 2+ dependency, is related to ExsB of Pseudomonas aeruginosa. Journal of Bacteriology, 177, 4230-4237.
5. BRICKER, B.J. (2002) Diagnostic strategies used for the identification of Brucella. Veterinary Microbiology, 90, 433-434.
6. CLOECKAERT, A., VIZCAINO, N., PAQUET, J-Y., BOWDEN, R.A. AND ELZER, P.H. (2002) Major outer membrane proteins of Brucella spp.: past, present and future. Veterinary Microbiology, 90, 229-247.
7. CORBEL, M.J. AND CULLEN, G.A. (1970) Differentiation of the serological response to Yersinia enterocolitica and Brucella abortus in cattle. Journal of Hygiene, 68, 519-530.
8. DECRETO MINISTERIALE 2 luglio 1992, n. 453: "Regolamento concernente il piano nazionale per la eradicazione della brucellosi negli allevamenti ovini e caprini".
9. DECRETO MINISTERIALE 27 agosto 1994, n. 651: "Regolamento concernente il piano nazionale per la eradicazione della brucellosi negli allevamenti bovini".
10. DUFEY, J. (1992) Le dépistage confronté aux réalités du terrain. Annales de Médecine Vétérinaire, 136, 281-283.
11. GARIN-BASTUJI, B., HUMMEL, N. GERBIER, G., CAU, C., POUILLOT, R., DA COSTA, M. AND FONTAINE, J.J. (1999) Non specific serological reactions in the diagnosis of bovine brucellosis: experimental oral infection of cattle with repeated doses of Yersinia enterocolitica 0:9. Veterinary Microbiology, 66, 223-233.
12. GODFROID, J., SAEGERMAN, C., WELLEMANS, V., WALRAVENS, K., LETESSON, J-J., TIBOR, A., Mc MILLAN, A., SPENCER, S., SANNA, M., BAKKER, D., POUILLOT, R. AND GARIN-BASTUJI, B. (2002). How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing. Veterinary Microbiology, 90, 461-477.
13. HEMMEN, F., WEYNANTS, V., SCARCEZ, T., LETESSON J.-J. AND SAMAN, E. (1995). Cloning and sequence analysis of a newly identified Brucella abortus gene and serological evaluation of the 17-kilodalton antigen that it encodes. Clinical and Diagnostic Laboratory Immunology, 2, 263-267.
14. KITTELBERGER, R., HILBINK, F., HANSEN, M.F., PENROSE, M., DE LISLE, G.W., LETESSON, J.-J., GARIN-BASTUJI, B., SEARSON, J., FOSSATI, C.A., CLOECKAERT, A., AND SCHURIG, G. (1995) Serological crossreactivity between Brucella abortus and Yersinia enterocolitica 0:9. I Immunoblot analysis of the antibody response to Brucella protein antigens in bovine brucellosis. Veterinary Microbiology, 47, 257-270.
15. LAEMMLI U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
16. MICHAUX-CHARACHON, S., FOULONGNE, V., O'CALLAGHAN D. AND RAMUZ M. (2002) Brucella à l'aube du troisième millénaire: organisation du génome et pouvoir pathogène. Pathologie Biologie, 50, 401-412.
17. NIELSEN, K. (2002) Diagnosis of brucellosis by serology. Veterinary Microbiology, 90, 447-459.
18. SAMBROOK, J., FRITSCH, E. F., AND MANIATIS, T. (1989) Plasmid vectors. In: Molecular cloning: a laboratory manual, Second Edition. Plainview, New York, Cold Spring Harbor Laboratory Press.
19. SANTOS, J. M. VERSTREATE, D.R., PERERA, V.Y. AND WINTER, A.J. (1984). Outer membrane proteins from rough strains of four Brucella species. Infection and Immunity, 46, 188-194.
20. THIBODEAU, V., FROST, E.H. AND QUESSY, S. (2001) Development of an ELISA procedure to detect swine carriers of pathogenic Yersinia enterocolitica. Veterinary Microbiology, 82, 249-259.
21. VERSTREATE, D.R AND WINTER, A.J. (1984). Comparison of Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Profiles and Antigenic Relatedness Among Outer Membrane Proteins of 49 Brucella abortus Strains. Infection and Immunity, 46, 182-187.
22. WEYNANTS, V., TIBOR, A., DENOEL, P.A., SAEGERMAN, C., GODFROID, J., THIANGE, P. AND LETESSON J.J. (1996). Infection of cattle with Yersinia enterocolitica 0:9 a cause of the false positive serological reactions in bovine brucellosis diagnostic tests. Veterinary Microbiology, 48, 101-12.