Recombining overlapping BACs into a single larger BAC

BMC Biotechnology

Volumn 4, Issue , 2004, Pages

  Kotzamanis, George ^a   Huxley, Clare ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; CHLORAMPHENICOL; CONTIG; ENDONUCLEASE; GENTAMICIN; SPECTINOMYCIN; TRANSMEMBRANE CONDUCTANCE REGULATOR; CFTR PROTEIN, HUMAN; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; BACTERIAL STRAIN; CHROMOSOME IDENTIFICATION; CLONING VECTOR; CONTROLLED STUDY; ELECTROPORATION; EXON; GENE INSERTION; GENE SEQUENCE; GENETIC TRANSCRIPTION; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN GENOME; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROMOTER REGION; SEQUENCE HOMOLOGY; CONTIG MAPPING; GENE VECTOR; GENETICS; METHODOLOGY;

MAMMALIA;

CHROMOSOMES, ARTIFICIAL, BACTERIAL; CONTIG MAPPING; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; GENETIC VECTORS; HUMANS;

EID: 3042561446     PISSN: 14726750     EISSN: None     Source Type: Journal    
DOI: 10.1186/1472-6750-4-1     Document Type: Article

References (17)

1. Lee GR, Fields PE, Griffin TJ, Flavell RA: Regulation of the Th2 cytokine locus by a locus control region. Immunity 2003, 19:1-2.
2. Mburu P, Mustapha M, Varela A, Weil D, El-Amraoui A, Holme RH, Rump A, Hardisty RE, Blanchard S, Coimbra RS, Perfettini I, Parkinson N, Mallon AM, Glenister P, Rogers MJ, Paige AJ, Moir L, Clay J, Rosenthal A, Liu XZ, Blanco G, Steel KP, Petit C, Brown S: Defects in whirlin, a PDZ domain molecule involved in stereocilia elongation, cause deafness in the whirler mouse and families with DFNB31. Nat Genet 2003, 34:421-428.
3. Narayanan K, Williamson R, Zhang Y, Stewart AF, Ioannou PA: Efficient and precise engineering of a 200 kb b-globin human/bacterial artificial chromosome in E. coli DH10B using an inducible homlogous recombination system. Gene Ther 1999, 6:442-447.
4. Copeland NG, Jenkins NA, Court DL: Recombineering: a powerful new tool for mouse functional genomics. Nat Rev Genet 2001, 2:769-779.
5. Muyrers JPP, Zhang Y, Stewart AF: Techniques: recombinogenic engineering - new options for cloning and manipulating DNA. Trends Biochem Sci 2001, 26:327-331.
6. Yu D, Ellis HM, Lee E-Chiang, Jenkins NA, Copeland NG, Court DL: An efficient recombination system for chromosome engineering in Escherichia coli. Proc Natl Acad Sci U S A 2000, 97:5978-5983.
7. Lee E-C, Yu D, de Velasco JM, Tessarollo L, Swing DA, Court DL, Jenkins NA, Copeland NG: A highly efficient Escherichia colibased chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA. Genomics 2001, 73:56-65.
8. Zhang X-M, Huang J-D: Combination of overlapping bacterial artificial chromosomes by a two-step recombinogenic engineering method. Nucleic Acids Res 2003, 31:e81.
9. Muyrers JPP, Zhang Y, Testa G, Stewart AF: Rapid modification of bacterial artificial chromosomes by ET-recombination. Nucleic Acids Res 1999, 27:1555-1557.
10. Zhang Y, Buchholz F, Muyrers JPP, Stewart AF: A new logic for DNA engineering using recombination in Escherichia coli. Nat Genet 1998, 20:123-128.
11. Liu P, Jenkins NA, Copeland NG: A highly efficient recombineering-based method for generating conditional knockout mutations. Genome Res 2003, 13:476-484.
12. Wittmayer PK, McKenzie JL, Raines RT: Degenerate DNA recognition by I-PpoI endonuclease. Gene 1998, 206:11-21.
13. Mejia JE, Larin Z: The assembly of large BACs by in vivo recombination. Genomics 2000, 70:165-170.
14. Kim U-J, Birren BW, Slepak T, Mancino V, Boysen C, Kang H-L, Simon MI, Shizuya H: Construction and characterization of a human bacterial artificial chromosome library. Genomics 1996, 34:213-218.
15. Magin-Lachmann C, Kotzamanis G, D'Aiuto L, wagner E, Huxley C: Retrofitting BACs with G418 resistance, luciferase, and oriP and EBNA-1 - new vectors for in vitro and in vivo delivery. BMC Biotechnology 2003, 3:2.
16. Alexeyev MF, Shokolenko IN, Croughan TP: Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector construction and in vitro deletion/insertion mutagenesis. Gene 1995, 160:63-67.
17. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning : A Laboratory Manual. 2ndth edition. Cold Spring Harbor, New York, Cold Spring Harbor Laboratory; 1989.