Efficient and precise engineering of a 200 kb β-globin human/bacterial artificial chromosome in E. coli DH10B using an inducible homologous recombination system

Gene Therapy

Volumn 6, Issue 3, 1999, Pages 442-447

  Narayanan, K ^a,b   Williamson, R ^a   Zhang, Y ^c   Stewart, A F ^c   Ioannou, P A ^a,d  

^a ROYAL CHILDREN'S HOSPITAL   (Australia)

^b UNIVERSITI MALAYSIA SARAWAK   (Malaysia)

^c EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^d CYPRUS INSTITUTE OF NEUROLOGY AND GENETICS   (Cyprus)

Author keywords

globin; BAC; Homologous recombination; PAC; recE; recT

Indexed keywords

BETA GLOBIN;

ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE CLUSTER; GENE MUTATION; GENE REARRANGEMENT; GENE SEQUENCE; GENETIC ENGINEERING; GENETIC RECOMBINATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPORTER GENE; YEAST ARTIFICIAL CHROMOSOME;

CLONING, MOLECULAR; ELECTROPORATION; ESCHERICHIA COLI; GENETIC ENGINEERING; GENETIC VECTORS; GLOBINS; HUMANS; POLYMERASE CHAIN REACTION;

EID: 0033002084     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3300901     Document Type: Article

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