Nitrification in activated sludge batch reactors is linked to protozoan grazing of the bacterial population

Canadian Journal of Microbiology

Volumn 51, Issue 9, 2005, Pages 791-799

  Petropoulos, Penny ^a   Gilbride, Kimberley A ^a  

^a RYERSON UNIVERSITY   (Canada)

Author keywords

Activated sludge; Ammonia oxidizing bacteria; Cycloheximide; Nitrification; Protozoan grazing

Indexed keywords

ACTIVATED SLUDGE PROCESS; AMMONIA; BACTERIA; NITRATES; NITRIFICATION; OXIDATION;

ACTIVATED SLUDGE; AMMONIA-OXIDIZING BACTERIA; CYCLOHEXIMIDE; PROTOZOAN GRAZING;

BIOREACTORS;

AMMONIA; CYCLOHEXIMIDE; NITRATE; NITRITE; NITROGEN;

ACTIVATED SLUDGE; BACTERIUM; INTERSPECIFIC INTERACTION; MICROBIAL ACTIVITY; NITRIFICATION; PROTOZOAN;

ACTIVATED SLUDGE; AMOEBA; ARTICLE; BACTERIAL COUNT; BACTERIUM; BATCH REACTOR; BIOCHEMICAL OXYGEN DEMAND; CHEMICAL OXYGEN DEMAND; CILIATE; CONTROLLED STUDY; FLAGELLATE; NITRIFICATION; NONHUMAN; NUTRIENT; PRIORITY JOURNAL; PROTOZOON; WASTE WATER; WATER SAMPLING;

AMMONIA; ANIMALS; BACTERIA; BIOREACTORS; COLONY COUNT, MICROBIAL; CYCLOHEXIMIDE; NITRITES; PROTOZOA; SEWAGE;

BACTERIA (MICROORGANISMS); CILIOPHORA; MASTIGOPHORA (FLAGELLATES); NITROSOMONADALES; PROTOZOA;

EID: 30344457573     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/W05-069     Document Type: Article

References (37)

1. Albright, L.J., Sherr, E.B., Sherr, B.R, and Fallon, RD. 1987. Grazing of ciliated Protozoa on free and particle-attached bacteria. Mar. Ecol. Prog. Ser. 38: 125-129.
2. Al-Shahawani, S.M., and Horan, N.J. 1991. The use of Protozoa to indicate changes in the performance of activated sludge plants. Water Res. 25: 633-638.
3. American Public Health Association. 1998. Standard methods for the examination of water and wastewater. 20th ed. American Public Health Association, New York.
4. Blackall, L.L., and Burrell, P.C. 1999. The microbiology of nitrogen removal in activated sludge systems. In The microbiology of activated sludge seviour. Edited by R.J and L.L. Blackall. Kluwer Academic Press, Boston, Mass. pp. 204-226.
5. Clarholm, M. 1985. Interactions of bacteria, protozoa and plants leading to mineralization of soil nitrogen. Soil Biol. Biochem. 17: 181-187.
6. Coleman, D.C., Cole, C.V., Hunt, H.W., and Klein, D.A. 1978. Trophic interactions in soils as they affect energy and nutrient dynamics. Microb. Ecol. 4: 345-349.
7. Curds, C.R. 1975. Protozoa. In Ecological aspects of used-water treatment. Vol. 1. Edited by C.R. Curds and H.A. Hawkes. Academic Press, London, U.K. pp. 203-268.
8. Curds, C.R. 1982. The ecology and role of protozoa in aerobic sewage treatment processes. Annu. Rev. Microbiol. 36: 27-46.
9. Curds, C.R., Cockburn, A., and Vandyke, J.M. 1968. An experimental study of the role of ciliated protozoa in the activated sludge process. Water Pollut. Control, 67: 312-328.
10. Daims, H., Nielsen, J., Nielsen, PH., Schleifer, K.H., and Wagner, M. 2001. In situ characterization of Nitrospira-like nitrite oxidizing bacteria in activated sludge. Appl. Environ. Microbiol. 67: 5273-5284.
11. Esteban, G., Tellez, C., and Bautista, L.M. 1991. Dynamics of ciliated protozoa communities in activated sludge process. Water Res. 25: 967-972.
12. Franklin, R.B., Garland, J.L., Bolster, C.H., and Mills, A. 2001. Impact of dilution on microbial community structure and functional potential: comparison of numerical simulations and batch culture experiments. Appl. Environ. Microbiol. 67: 702-712.
13. Galiano, D.F. 1994. The ammonical silver carbonate method as a general procedure in the study of protozoa from sewage (and other) waters. Water Res. 28: 495-496.
14. Hagopian, D.S., and Riley, J.G. 1998. A closer look at bacteriology of nitrification. Aquacult. Eng. 18: 223-244.
15. Juretschko, S., Timmermann, G., Schmid, M., Schleifer, K.H., Pommerening-Roser, A., Koops, H.P., and Wagner, M. 1998. Combined molecular and conventional analysis of nitrifying bacteria diversity in activated sludge: Nitrosococcus mobilis and Nitrospira-like bacteria as dominant populations. Appl. Environ. Microbiol. 64: 3042-3050.
16. Madigan, M.T., Martinko, J.M., and Parker, J. 2000. Brock biology of microorganisms. Prentice Hall, Upper Saddle River, N.J.
17. Madony, P., Davoli, D., and Chierici, E. 1993. Comparative analysis of the activated sludge microfauna in several sewage treatment works. Water Res. 27: 1485-1491.
18. Mobarry, B.K., Wagner, M., Urbain, V., and Ritmann, B.E. 1996. Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria. Appl. Environ. Microbiol. 62: 2156-2162.
19. Nisbet, B. 1984. Nutrition and feeding strategies in protozoa. Croom Helm, London, U.K.
20. Petropoulos, P. 2003. The interaction of bacteria and protozoa in nitrogen cycling in activated sludge process. M.Sc. thesis, Teesside University, U.K.
21. Princic, A., Mahne, I., Megusar, F., Paul, E.A., and Tiedje, J.M. 1998. Effect of pH and oxygen and ammonium concentrations on the community structure of nitrifying bacteria from waste-water. Appl. Environ. Microbiol. 64: 3584-3590.
22. Ratsak, C.H., Maarsen, K.A., and Kooijman, S.A.L.M. 1996. Effects of protozoa on carbon mineralization in activated sludge. Water Res. 30: 1-12.
23. Sanders, R.W., and Porter, K.G. 1986. Use of metabolic inhibitors to estimate protozooplankton grazing and bacterial production in a monomictic eutrophic lake with an anaerobic hypolimnion. Appl. Environ. Microbiol. 52: 101-107.
24. Sherr, B.F., Sherr, E.B., and Hopkinson, C.S. 1988. Trophic interactions within pelagic microbial communities: indications of feedback regulation of carbon flow. Hydrobiologia, 159: 19-23.
25. Sinclair, J.L., and Alexander, M. 1989. Effect of protozoan predation on relative abundance of fast- and slow-growing bacteria. Can. J. Microbiol. 35: 578-582.
26. Stout, D. 1980. The role of protozoa in nutrient cycling and energy flow. Adv. Microb. Ecol. 4: 1-50.
27. Strauss, E.A., and Dodds, W.K. 1997. Influence of protozoa and nutrient availability on nitrification rates in substrate sediments. Microb. Ecol. 34: 155-165.
28. Varma, M.M., Finely, H.E., and Bennett, H. 1975. Population dynamics of protozoa in wastewater. J. Water Pollut. Control Fed. 47: 85-92.
29. Verhagen, F.J.M., and Laanbroek, H.G. 1992. Effects of grazing by flagellates on competition for ammonium between nitrifying and heterotrophic bacteria in chemostats. Appl. Environ. Microbiol. 58: 1962-1969.
30. Victorio, L. 1995. Characterization of microbial communities in wastewater treatment systems. M. Philos, thesis, University of Teesside, Middlesbrough, U.K.
31. Victorio, L., Allen, D.G., Gilbride, K.A., and Liss, S.N. 1996. Rapid monitoring of metabolic and biodegradation activity of microbial communities in wastewater treatment systems. Water Res. 30: 1077-1086.
32. Viessman, W., Jr., and Hammer, M.J. 1998. Water supply and pollution control. Addison Wesley Longman Inc., Don Mills, Ontario.
33. Wagner, M., and Loy, A. 2002. Bacterial community composition and function in sewage treatment plants. Curr. Opin. Biotechnol. 13: 218-227.
34. Wagner, M., Rath, G., Amann, R., Koops, H.P., and Schleifer, K.H. 1995. In situ identification of ammonia oxidizing bacteria. Syst. Appl. Microbiol. 18: 251-264.
35. Wagner, M., Rath, G., Koops, H.-P., Flood, J., and Amann, R. 1996. In situ analyses of nitrifying bacteria in sewage treatment plants. Water Sci. Technol. 34: 237-244.
36. Wagner, M., Noguera, D., Juretschko, S., Rath, G., Koops, H.-P., and Schleifer, K.H. 1998. Combining FISH with cultivation and mathematical modeling to study population structure and function of ammonia oxidizing bacteria in activated sludge. Water Sci. Technol. 37: 441-449.
37. Wheale, G., and Williamson, D.J. 1980. Unusual behaviour of ciliated protozoa in a secondary settlement tank. Water Pollut. Control, 80: 496-500.