Truncated HP1 lacking a functional chromodomain induces heterochromatinization upon in vivo targeting

Histochemistry and Cell Biology

Volumn 125, Issue 1-2, 2006, Pages 53-61

  Brink, Maartje C ^a   Van Der Velden, Yme ^a   De Leeuw, Wim ^b   Mateos Langerak, Julio ^a   Belmont, Andrew S ^c   Van Driel, Roel ^a   Verschure, Pernette J ^a  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b CWI   (Netherlands)

^c UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

Epigenetics; Gene expression; Heterochromatin protein 1; Histone methylation; Large scale chromatin structure

Indexed keywords

HETEROCHROMATIN PROTEIN 1; HISTONE; HISTONE H3; LYSINE;

ANIMAL CELL; ARTICLE; CHROMATIN CONDENSATION; CHROMOSOME; DEACETYLATION; GENE AMPLIFICATION; GENE EXPRESSION; HETEROCHROMATIN; LACTOSE OPERON; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN METHYLATION;

ANIMALS; CHO CELLS; CHROMATIN; CHROMOSOMAL PROTEINS, NON-HISTONE; CRICETINAE; FLUORESCENT ANTIBODY TECHNIQUE; HETEROCHROMATIN; HISTONES; HUMANS; IMAGE INTERPRETATION, COMPUTER-ASSISTED; LYSINE; METHYLATION; MICROSCOPY, CONFOCAL; PLASMIDS;

ANIMALIA; EUKARYOTA; MAMMALIA;

EID: 30044434949     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00418-005-0088-7     Document Type: Article

References (34)

1. Aasland R, Stewart AF (1995) The chromo shadow domain, a second chromo domain in heterochromatin-binding protein 1, HP1. Nucleic Acids Res 23: 3168-3173
2. Badugu R, Yoo Y, Singh PB, Kellum R. (2005) Mutations in the heterochromatin protein 1 (HP1) hinge domain affect HP1 protein interactions and chromosomal distribution. Chromosoma 113: 370-384
3. Bannister AJ, Zegerman P, Partridge JF, Miska EA, Thomas JO, Allshire RC, Kouzarides T (2001) Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain. Nature 410: 120-124
4. Cheutin T, McNairn AJ, Jenuwein T, Gilbert DM, Singh PB, Misteli T (2003) Maintenance of stable heterochromatin domains by dynamic HP1 binding. Science 299: 721-725
5. Cowell IG, Aucott R, Mahadevaiah SK, Burgoyne PS, Huskisson N, Bongiorni S, Prantera G, Fant L, Pimpinelli S, Wu R, Gilbert DM, Shi W, Fundele R, Morrison H, Jeppesen P, Singh PB (2002) Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals. Chromosoma 111: 22-36
6. Dillon N (2004) Heterochromatin structure and function. Biol Cell 96: 631-637
7. Horn PJ, Peterson CL (2002) Molecular biology: Chromatin higher order folding: Wrapping up transcription. Science 297: 1824-1827
8. Horsley D, Hutchnigs A, Butcher GW, Singh PB (1996) M32, a murine homologue of Drosophila heterochromatin protein 1 (HP1), localises to euchromatin within interphase nuclei and is largely excluded from constitutive heterochromatin. Cytogenet Cell Genet 73: 308-311
9. Jacobs SA, Khorasanizadeh S (2002) Structure of HP1 chromodomain bound to a lysine 9-methylated histone H3 tail. Science 295: 2080-2083
10. Jacobs SA, Taverna SD, Zhang Y, Briggs SD, Li J, Eissenberg JC, Allis CD, Khorasanizadeh S (2001) Specificity of the HP1 chromo domain for the methylated N-terminus of histone H3. Embo J 20: 5232-5241
11. Lachner M, Jenuwein T (2002) The many faces of histone lysine methylation. Curr Opin Cell Biol 14: 286-298
12. Lachner M, O'Carroll D, Rea S, Mechtler K, Jenuwein T (2001) Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins. Nature 410: 116-120
13. Lechner MS, Schultz DC, Negorev D, Maul GG, Rauscher FJ3rd (2005) The mammalian heterochromatin protein 1 binds diverse nuclear proteins through a common motif that targets the chromoshadow domain. Biochem Biophys Res Commun 331: 929-937
14. Li YH, Danzer JR, Alvarez P, Belmont AS, Wallrath LL (2003) Effects of tethering HP1 to euchromatic regions of the Drosophila genome. Development 130: 1817-1824
15. Li YH, Kirschmann DA, Wallrath LL (2002) Does heterochromatin protein 1 always follow code? Proc Nat Acad Sci USA 99: 16462-16469
16. Maison C, Almouzni G (2004) HP1 and the dynamics of heterochromatin maintenance. Nat Rev Mol Cell Biol 5: 296-304
17. Meehan RR, Kao CF, Pennings S (2003) HP1 binding to native chromatin in vitro is determined by the hinge region and not by the chromodomain. Embo J 22: 3164-3174
18. Minc E, Allory V, Worman HJ, Courvalin JC, Buendia B (1999) Localization and phosphorylation of HP1 proteins during the cell cycle in mammalian cells. Chromosoma 108: 220-234
19. Muchardt C, Guilleme M, Seeler JS, Trouche D, Dejean A, Yaniv M (2002) Coordinated methyl and RNA binding is required for heterochromatin localization of mammalian HP1 alpha. Embo Rep 3: 975-981
20. Nakayama J, Rice JC, Strahl BD, Allis CD, Grewal SI (2001) Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly. Science 292: 110-113
21. Nielsen AL, OuladAbdelghani M, Ortiz JA, Remboutsika E, Chambon P, Losson R (2001) Heterochromatin formation in mammalian cells: Interaction between histones and HP1 proteins. Mol Cell 7: 729-739
22. Nielsen PR, Nietlispach D, Mott HR, Callaghan J, Bannister A, Kouzarides T, Murzin AG, Murzina NV, Laue ED (2002) Structure of the HP1 chromodomain bound to histone H3 methylated at lysine 9. Nature 416: 103-107
23. Nye AC, Rajendran RR, Stenoien DL, Mancini MA, Katzenellenbogen BS, Belmont AS (2002) Alteration of large-scale chromatin structure by estrogen receptor. Mol Cell Biol 22: 3437-3449
24. Platero JS, Hartnett T, Eissenberg JC (1995) Functional analysis of the chromo domain of HP1. Embo J 14: 3977-3986
25. Rice JC, Briggs SD, Ueberheide B, Barber CM, Shabanowitz J, Hunt DF, Shinkai Y, Allis CD (2003) Histone methyltransferases direct different degrees of methylation to define distinct chromatin domains. Mol Cell 12: 1591-1598
26. Robinett CC, Straight A, Li G, Willhelm C, Sudlow G, Murray A, Belmont AS (1996) In vivo localization of DNA sequences and visualization of large-scale chromatin organization using lac operator/repressor recognition. J Cell Biol 135: 1685-1700
27. Smothers JF, Henikoff S (2001) The hinge and chromo shadow domain impart distinct targeting of HP1- like proteins. Mol Cell Biol 21: 2555-2569
28. Stewart MD, Li J, Wong J (2005) Relationship between histone H3 lystine 9 methylation, transcription repression and heterochromatin protein 1 recruitment. Mol Cell Biol 21: 2525-2538
29. Thiru A, Nietlispach D, Mott HR, Okuwaki M, Lyon D, Nielsen PR, Hirshberg M, Verreault A, Murzina NV, Laue ED (2004) Structural basis of HP1/PXVXL motif peptide interactions and HP1 localisation to heterochromatin. EMBO J 23: 489-499
30. Tumbar T, Sudlow G, Belmont AS (1999) Large-scale chromatin unfolding and remodeling induced by VP16 acidic activation domain. J Cell Biol 145: 1341-1354
31. Verschure PJ, Van Der Kraan I, Enserink JM, Mone MJ, Manders EM, Van Driel R (2002) Large-scale chromatin organization and the localization of proteins involved in gene expression in human cells. J Histochem Cytochem 50: 4552-4564
32. Verschure PJ (2004) Positioning the genome within the nucleus. Biol Cell 25: 96: 569-577
33. Verschure PJ, Van Der Kraan I, De Leeuw W, Van der Vlag J, Carpenter AE, Belmont AS, Van Driel R (2005) In vivo HP1 targeting causes large-scale chromatin condensation and enhanced histone lysine methylation. Mol Cell Biol 25: 4552-4564
34. Wreggett KA, Hill F, James PS, Hutchings A, Butcher GW, Singh PB (1994) A Mammalian Homologue of Drosophila Heterochromatin Protein 1 (HP1) is a component of constitutive heterochromatin. Cytogenetics Cell Genet 66: 99-103