Parallel analysis of mutant human glucose 6-phosphate dehydrogenase in yeast using PCR colonies

Biotechnology and Bioengineering

Volumn 92, Issue 5, 2005, Pages 519-531

  Merritt, Joshua ^a   Butz, James A ^a   Ogunnaike, Babatunde A ^a   Edwards, Jeremy S ^b  

^a University of Delaware   (United States)

^b UNIVERSITY OF NEW MEXICO HEALTH SCIENCES CENTER   (United States)

Author keywords

Functional complementation; Glucose 6 phosphate dehydrogenase; High throughput sequencing; Polonies

Indexed keywords

ENZYME KINETICS; GENETIC ENGINEERING; GLUCOSE; PHOSPHATES; THROUGHPUT; YEAST;

FUNCTIONAL COMPLEMENTATION; GLUCOSE 6-PHOSPHATE DEHYDROGENASE; HIGH THROUGHPUT SEQUENCING; POLONIES;

MUTAGENESIS;

ARGININE; ASPARTIC ACID; BINDING PROTEIN; GLUCOSE 6 PHOSPHATE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; GLYCINE; HISTIDINE; ISOLEUCINE; LEUCINE; LYSINE; MUTANT PROTEIN; NUCLEOTIDE; TYROSINE;

AMINO ACID SUBSTITUTION; ARTICLE; ENZYME ANALYSIS; FUNGAL GENE; FUNGAL STRAIN; FUNGUS GROWTH; GENE DELETION; GENE MUTATION; GENETIC COMPLEMENTATION; MISSENSE MUTATION; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; TECHNIQUE; YEAST; ZWF1 GENE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; DNA MUTATIONAL ANALYSIS; GLUCOSEPHOSPHATE DEHYDROGENASE; HUMANS; MOLECULAR SEQUENCE DATA; MUTATION; PEPTIDE LIBRARY; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ANALYSIS, PROTEIN;

FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 29544450052     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20726     Document Type: Article

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