P450scc mutant nanostructuring for optimal assembly

IEEE Transactions on Nanobioscience

Volumn 3, Issue 2, 2004, Pages 121-128

  Ghisellini, Paola ^a   Paternolli, Cristina ^a   Antonini, Mirco ^a   Nicolini, Claudio ^a  

^a UNIVERSITY OF GENOA   (Italy)

Author keywords

Cytochrome P450scc; Langmuir Blodgett (LB) technique; Molecular modeling; Mutagenesis

Indexed keywords

BIOSENSORS; BIOSYNTHESIS; ELECTRON TRANSITIONS; ENZYMES; HYDROPHOBICITY; LANGMUIR BLODGETT FILMS; MUTAGENESIS; PROTEINS; SOLVENTS; THERMODYNAMIC STABILITY; ULTRAVIOLET SPECTROSCOPY;

CYTOCHROME P450SCC; MOLECULAR MODELING; PROTEIN IMMOBILIZATION; TOXICOLOGY;

NANOSTRUCTURED MATERIALS;

CYTOCHROME P450;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BIOSYNTHESIS; CHEMICAL STRUCTURE; CHEMISTRY; COMPUTER SIMULATION; ELECTROCHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETIC ENHANCEMENT; GENETICS; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NANOTECHNOLOGY; PROTEIN CONFORMATION; PROTEIN ENGINEERING; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; TEMPERATURE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CLONING, MOLECULAR; COMPUTER SIMULATION; CYTOCHROME P-450 ENZYME SYSTEM; ELECTROCHEMISTRY; ENZYME STABILITY; ESCHERICHIA COLI; GENETIC ENHANCEMENT; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NANOTECHNOLOGY; PROTEIN CONFORMATION; PROTEIN ENGINEERING; SEQUENCE ANALYSIS, PROTEIN; STRUCTURE-ACTIVITY RELATIONSHIP; TEMPERATURE;

EID: 2942659622     PISSN: 15361241     EISSN: None     Source Type: Journal    
DOI: 10.1109/TNB.2004.828267     Document Type: Article

References (47)

1. F. J. Gonzalez, "The molecular biology of cytochrome P450s," Pharmacol. Rev., vol. 40, pp. 243-288, 1988.
2. M. R. Waterman and E. R. Simpson, "Regulation of the biochemical of cytochromes P450 involved in steroid hormone synthesis," Mol. Cell. Endocrinol., vol. 39, pp. 81-89, 1995.
3. J. D. Lambeth, "Cytochrome P-450scc: A review of the specificity and properties of the cholesterol binding site," Endocr. Res., vol. 12, pp. 371-392, 1986.
4. M. Morisaki, C. Duque, N. Ikekawa, and M. Shikita, "Substrates specifity of adrenocortical cytochrome P450scc. I. Effects of structural modification of cholesterol side chain on pregnenolone production," J. Steroid Biochem., vol. 12, pp. 545-550, 1980.
5. V. M. Shkumatov, S. A. Usanov, V. L. Chashchin, and A. A. Akhrem, "Cytochrome P450 dependent pathways in corticosteroid hormones biosynthesis," Pharmazie, vol. 40, pp. 757-766, 1985.
6. J. D. Lambeth, L. M. Geren, and F. Millett, "Adrenodoxin interaction with adrenodoxin reductase and cytochrome P450scc. Cross-linking of protein complexes and effects of adrenodoxin modification by 1-ethyl-3-(3- dimethylaminopropyl)carbodiimide," J. Biol. Chem., vol. 259, pp. 10 025-10 029, 1984.
7. C. Nicolini, "Protein monolayer engineering: Principles and application to biocatalysis," Trends Biotechnol., vol. 15, pp. 395-401, 1997.
8. _, Molecular Manufacturing, ser. EL.B.A. Forum Series, C. Nicolini, Ed. New York: Plenum, 1996, vol. 2, ch. 1.
9. G. Roberts, Langmuir-Blodgett Films. New York: Plenum, 1990.
10. A. Ulman, An Introduction to Ultrathin Organic Films: From Langmuir-Blodgett to Self-Assembly. New York: Academic, 1991.
11. M. Antonini, P. Ghisellini, L. Pastorino, C. Paternolli, and C. Nicolini, "Preliminary electrochemical characterization of cytochrome P4501A2-clozapine interaction," IEE Proc. - Nanobiotechnol., vol. 150, pp. 31-34, 2003.
12. C. Paternolli, P. Ghisellini, and C. Nicolini, "Development of immobilization techniques of cyotchrome P450-GST fusion protein," Colloids Surf. B, vol. 23, pp. 305-311, 2002.
13. _, "Pollutant sensing layer based on cytochrome P450," Mater. Sci. Eng. C, vol. 22, pp. 155-159, 2002.
14. O. Guryev, V. Erokhin, S. Usanov, and C. Nicolini, "Cytochrome P450scc spin state transitions in the solid films," Biochem. Mol. Biol. Int., vol. 39, pp. 205-214, 1996.
15. P. Ghisellini, C. Paternolli, I. Chiossone, and C. Nicolini, "Spin state transitions in Langmuir-Blodgett films of recombinant cytochrome P450scc and adrenodoxin," Colloids Surf. B, vol. 23, pp. 313-318, 2002.
16. V. Erokhin, S. Carrara, S. Guerzoni, P. Ghisellini, and C. Nicolini, "Synchrotron study of heat induced order in protein LB films," Thin Solid Films, vol. 327, pp. 636-638, 1998.
17. C. Nicolini, "From protein engineering to bioelectronics," in From Neural Networks and Biomolecular Engineering to Bioelectronics. ser. EL.B.A. Forum Series, C. Nicolini, Ed. New York: Plenum, 1995, vol. 1, pp. 1-36.
18. _, "Molecular manufacturing: A new frontier at the interface of electronics, biotechnology and material sciences," in Molecular Manufacturing. ser. EL.B.A. Forum Series, C. Nicolini, Ed. New York: Plenum, 1996, vol. 2, pp. 1-51.
19. S. Vijayakumar and J. C. Salerno, "Molecular modeling of the 3-D structure of cytochrome P-450scc," Biochim. Biophys. Acta., vol. 1160, pp. 281-286, 1992.
20. H. M. Berman, J. Westbrook, Z. Feng, G. Gilland, T. N. Bhat, H. Weissig, I. N. Shindyalov, and P. E. Bourne, "The protein data bank and the challenge of structural genomics," Nucleic Acid Res., vol. 28, pp. 235-242, 2000.
21. T. L. Poulos, B. C. Finzel, and A. J. Howard, "High-resolution crystal structure of cytochrome P450cam," J. Mol. Biol., vol. 195, pp. 687-700, 1987.
22. V. S. Sivozhelezov, "The role of electrostatics in the interactions of some globular protein," Ph.D. dissertation, Inst. Cell Biophys., Russian Acad. Sci., Pushchino, Russia, 1996.
23. R. Koradi, M. Billeter, and K. Wüthrich, "MOLMOL: A program for display and analysis of macromolecular structures," J. Mol. Graph., vol. 14, pp. 51-55, 1996.
24. L. E. Vickery, "Molecular recognition and electron transfer in mitochondrial steroid hydroxylase systems," Steroids, vol. 62, pp. 124-127, 1997.
25. A. Wada, P. A. Mathew, H. J. Barnes, D. Sanders, R. W. Estabrook, and M. R. Waterman, "Expression of functional bovine cholesterol side chain cleavage cytochrome P450 (P450scc)," Escherichia Coli, Arch. Biochem. Biophys., vol. 290, pp. 376-380, 1991.
26. R. N. Dubois, E. R. Simpson, J. Tuckey, J. D. Lambeth, and M. R. Waterman, "Evidence for a higher molecular weight precursor of cholesterol side-chain-cleavage cytochrome P-450 and induction of mitochondrial and cytosolic proteins by corticotropin in adult bovine adrenal cells," in Proc. Nat. Acad. Sci., vol. 78, 1981, pp. 1028-1032.
27. M. M. Bradford, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding," Anal. Biochem., vol. 72, pp. 248-254, 1976.
28. U. Laemmli, "Cleavage of structural proteins during the assembly of the head of bacteriophage T4," Nature, vol. 227, pp. 680-685, 1970.
29. T. Omura and R. Sato, "The carbon monooxide-binding pigment of liver microsomes: I. Evidence for its hemoprotein nature," J. Biol. Chem., vol. 239, pp. 2370-2378, 1984.
30. V. Erokhin, P. Facci, and C. Nicolini, "Two-dimensional order and protein thermal stability: High temperature preservation of structure and function," Biosens. Bioelectron., vol. 10, pp. 25-34, 1995.
31. G. Z. Sauerbrey, "The use of quartz oscillators for weighing thin layers and for microweighing," Z. Phisik., vol. 155, pp. 206-222, 1959.
32. C. K. O'Sullivan and G. G. Guilbault, "Commercial quartz crystal microbalances - Theory and applications," Biosens. Bioelectron., vol. 14, pp. 663-670, 1999.
33. P. Facci, V. Erokhin, and C. Nicolini, "Nanogravimetric gauge for surface density measurements and deposition analysis of LB films," Thin Solid Films, vol. 230, pp. 86-89, 1993.
34. F. Antolini, S. Paddeu, and C. Nicolini, "Heat-stable Langmuir-Blodgett film of Glutathione-S-transferase," Langmuir, vol. 11, no. 7, pp. 2719-2725, 1995.
35. S. Paddeu, A. Fanigliuolo, M. Lanzi, T. Dubrovski, and C. Nicolini, "LB-based PAB immunosystem: Activity of immobilized urease monolayer," Sens. Actuators B, vol. 25, no. 1-3, pp. 876-882, 1995.
36. P. K. Weiner and P. A. Kollman, "AMBER: Assisted model building with energy refinement. A general program for modeling molecules and their interactions," J. Comp. Chem., vol. 2, pp. 287-303, 1981.
37. G. E. Schulz and R. H. Schirmer, Principles of Protein Structure. New York: Springer-Verlag, 1979.
38. B. Honig and A. Nicolls, "Classical electrostatics in biology and chemistry," Science, vol. 268, pp. 1144-1149, 1995.
39. J. Tuls, L. Geren, and F. Millett, "Fluorescent isothiocyanate specifically modifies lysine 338 of cytochrome P-450scc and inhibits adrenodoxin binding," J. Biol. Chem., vol. 264, pp. 16421-16425, 1989.
40. C. Nicolini, V. Erokhin, P. Ghisellini, C. Paternolli, K. Ram, and V. Sivozelhezov, "P450scc engineering and nanostructuring for cholesterol sensing," Langmuir, vol. 17, pp. 3719-3726, 2001.
41. B. Nölting, C. Jung, and G. Snatzke, "Multichannel circular dichroism investigations of the structural stability of bacterial cytochrome P-450," Biochem. Biophys. Acta, vol. 1100, no. 2, pp. 171-176, 1992.
42. C. Nicolini, V. Erokhin, F. Antolini, P. Catasti, and P. Facci, "Thermal stability of protein secondary structure in Langmuir-Blodgett films," Biochem. Biophys. Acta., vol. 1158, pp. 273-278, 1993.
43. P. Facci, V. Erokhin, and C. Nicolini, "Scanning tunneling microscopy of a monolayer of reaction centers," Thin Solid Films, vol. 243, pp. 403-406, 1994.
44. L. A. Andersson, M. Sono, and J. H. Dawson, "Circular dichroism studies of low-spin ferric cytochrome P-450CAM ligand complexes," Biochem. Biophys. Acta., vol. 748, pp. 341-352, 1983.
45. S. Bartolucci, A. Gagliardi, E. Pedone, D. De Pascale, R. Cannio, L. Camardella, V. Carratore, M. Rossi, G. Nicastro, C. De Chiara, and C. Nicolini, "The thioredoxin from Bacillus acidocaldarius: Characterization, molecular modeling study and high-level expression in Escherichia coli," Biochem. J., vol. 328, pp. 277-285, 1997.
46. C. Nicolini, V. Erokhin, and M. K. Ram, "Supramolecular layer engineering for industrial nanotechnology," in Nano-Surface Chemistry, M. Rosoff, Ed. New York: Marcel Dekker, 2001, vol. 4, pp. 141-212.
47. C. Nicolini, "Metalloprotein engineering for new materials, drugs, and nanodevices," in Biophysics of Electron Transfer and Molecular Bioelectronics, ser. EL.B.A. Forum Series, C. Nicolini, Ed. New York: Plenum, 1998, vol. 3, ch. 1, pp. 1-32.