Microarrays of peptides elevated on the protein layer for efficient protein kinase assay

Analytical Biochemistry

Volumn 330, Issue 2, 2004, Pages 311-316

  Lee, Seok Jae ^a   Lee, Sang Yup ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Fusion protein; Kinase; Peptide microarray; Protein layer

Indexed keywords

BIOASSAY; BIOSYNTHESIS; CHEMICAL DETECTION; CHEMICAL MODIFICATION; COST EFFECTIVENESS; ENZYMES; ESCHERICHIA COLI; RECOMBINANT PROTEINS; SUBSTRATES;

CHEMICAL DERIVATIZATION; FUSION PROTEINS; HIGH-THROUGHPUT ANALYSIS; KINASE; PEPTIDE-PROTEIN INTERACTIONS; PROTEIN LAYERS; PROTEIN-PEPTIDE INTERACTIONS; RECOMBINANT ESCHERICHIA COLI;

PEPTIDES;

HYBRID PROTEIN; KEMPTIDE; PROTEIN KINASE;

ARTICLE; CHEMICAL MODIFICATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SUBSTRATE; ESCHERICHIA COLI; FERMENTATION; INTERMETHOD COMPARISON; PEPTIDE ANALYSIS; PEPTIDE MICROARRAY; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN MODIFICATION; PROTEIN PROTEIN INTERACTION; SENSITIVITY AND SPECIFICITY;

ESCHERICHIA COLI;

EID: 2942627618     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.04.008     Document Type: Article

References (15)

1. Cohen C.B., Chin-Dixon E., Jeong S., Nikiforov T.T. A microchip-based enzyme assay for protein kinase A. Anal. Biochem. 273:1999;89-97
2. Ahn N.G., Resing K.A. Toward the phosphoproteome. Nat. Biotechnol. 19:2001;317-318
3. MacBeath G., Schreiber S.L. Printing proteins as microarrays for high-throughput function determination. Science. 289:2000;1760-1763
4. MacBeath G., Koehler A.N., Schreiber S.L. Printing small molecules as microarrays and detecting protein-ligand interactions en masse. J. Am. Chem. Soc. 121:1999;7967-7968
5. Zhu H., Klemic J.F., Chang S., Bertone P., Casmayor A., Klemic K.G., Smith D., Gerstein M., Reed M.A., Snyder M. Analysis of yeast protein kinases using protein chips. Nat. Genet. 26:2000;283-289
6. Salisbury C.M., Maly D.J., Ellman J.A. Peptide microarrays for the determination of protease substrate specificity. J. Am. Chem. Soc. 124:2002;14868-14870
7. Reineke U., Volkmer-Engert R., Schneider-Mergener J. Applications of peptide arrays prepared by the SPOT-technology. Curr. Opin. Biotechnol. 12:2001;59-64
8. Houseman B.T., Huh J.H., Kron S.J., Mrksich M. Peptide chips for the quantitative evaluation of protein kinase activity. Nat. Biotechnol. 20:2002;270-274
9. Scott J.E., Carpenter J.W. A homogeneous assay of kinase activity that detects phosphopeptide using fluorescence polarization and zinc. Anal. Biochem. 316:2003;82-91
10. Wenschuh H., Volkmer-Engert R., Schmidt M., Schulz M., Schneider-Mergener J., Reineke U. Coherent membrane supports for parallel microsynthesis and screening of bioactive peptides. Biopolymers. 55:2000;188-206
11. Lesaicherre M., Uttamchandani M., Chen G.Y.J., Yao S.Q. Antibody-based fluorescence detection of kinase activity on a peptide array. Bioorg. Med. Chem. Lett. 12:2002;2085-2088
12. Jeong K.J., Lee S.Y. High-level production of human leptin by fed-batch cultivation of recombinant Escherichia coli and its purification. Appl. Environ. Microbiol. 65:1999;3027-3032
13. Sambrook J., Russell D.W. third ed. Molecular Cloning: A Laboratory Manual. vols. 1-3:2001;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
14. Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254
15. Yoon S.H., Choi J.G., Lee S.Y. Development of DNA microarrayer. J. Microbiol. Biotechnol. 10:2000;21-26