A rapid method for analyzing recombinant protein inclusion bodies by mass spectrometry

Analytical Biochemistry

Volumn 330, Issue 1, 2004, Pages 140-144

  Grimm, Rudolf ^a,c   Huber, Robert ^a   Neumeier, Thomas ^a   Seidl, Andreas ^a   Haslbeck, Martin ^b   Seibert, Fabian S ^a  

^a Sandoz Biopharmaceuticals   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^c AGILENT TECHNOLOGIES   (Germany)

Author keywords

Inclusion bodies; MALDI; Peptide mass fingerprint; Recombinant proteins; Urea

Indexed keywords

AMMONIA; DESALINATION; ESCHERICHIA COLI; MASS SPECTROMETRY; METABOLISM; PEPTIDES; UREA;

ELEVATED TEMPERATURE; INCLUSION BODIES; MALDI; MATRIX ASSISTED LASER DESORPTION IONIZATION; PEPTIDE MASS FINGERPRINT; PROTEIN MODIFICATIONS; RECOMBINANT PROTEIN EXPRESSION; TIME OF FLIGHT MASS SPECTROMETRY;

RECOMBINANT PROTEINS;

AMINO ACID; AMMONIA; BUFFER; RECOMBINANT PROTEIN; SODIUM ION; UREA;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL INCLUSION; FINGER DERMATOGLYPHICS; INCUBATION TIME; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN MODIFICATION; PROTEIN STRUCTURE; SAMPLE; TEMPERATURE;

ESCHERICHIA COLI;

EID: 2942604731     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.03.043     Document Type: Article

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