Fluorescence in situ hybridization using rRNA-targeted probes for simple and rapid identification of the toxic dinoflagellates Alexandrium tamarense and Alexandrium catenella

Journal of Phycology

Volumn 40, Issue 3, 2004, Pages 598-605

  Sako, Yoshihiko ^a   Hosoi Tanabe, Shoko ^a   Uchida, Aritsune ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Alexandrium; FISH method; LSU rRNA; Molecular identification; Paralytic shellfish poisoning (PSP); Probe; Toxic dinoflagellate

Indexed keywords

DINOFLAGELLATE; FLUORESCENCE; TOXIC ORGANISM;

AKASHIWO; ALEXANDRIUM; ALEXANDRIUM AFFINE; ALEXANDRIUM CATENELLA; ALEXANDRIUM INSUETUM; ALEXANDRIUM LUSITANICUM; ALEXANDRIUM OSTENFELDII; ALEXANDRIUM TAMARENSE; ALGAE; CATENELLA; DINOPHYCEAE; GYMNODINIUM; GYMNODINIUM MIKIMOTOI; HARA; HETEROSIGMA AKASHIWO;

EID: 2942562837     PISSN: 00223646     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1529-8817.2004.03035.x     Document Type: Article

References (28)

1. Adachi, M., Sako, Y. & Ishida, Y. 1993. Application of monoclonal antibodies to field samples of Alexandrium species. Nippon Suisan Gakkaishi 59:1171-5.
2. Adachi, M., Sako, Y. & Ishida, Y. 1994. Restriction fragment length polymorphism of ribosomal DNA internal transcribed spacer and 5.8S regions in Japanese Alexandrium species (Dinophyceae). J. Phycol. 30:857-63.
3. Adachi, M., Sako, Y. & Ishida, Y. 1996a. Analysis of Alexandrium (Dinophyceae) species using sequences of the 5.8S ribosomal DNA and internal transcribed spacer regions. J. Phycol. 32:424-32.
4. Adachi, M., Sako, Y. & Ishida, Y. 1996b. Identification of the toxic dinoflagellates Alexandrium catenella and A. tamarense (Dinophyceae) using DNA probes and whole-cell hybridization. J. Phycol. 32:1049-52.
5. Amann, R. I., Krumholz, L. & Stahl, D. A. 1990. Fluorescent- oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J. Bacteriol. 172:762-70.
6. Anderson, D. M. 1995. Identification of harmful algal species using molecular probes: an emerging technology. In Lassus, P., Arzul, G., Erard, E., Gentien, P. & Marcaillou, C. [Eds.] Harmful Marine Algal Blooms. Lavoiser Science Publishers, Paris, pp. 3-13.
7. Anderson, D. M., Kulis, D. M., Keafer, B. A. & Berdalet, E. 1999. Detection of the toxic dinoflagellate Alexandrium fundyense (Dinophyceae) with oligonucleotide and antibody probes: variation in labeling intensity with physiological condition. J. Phycol. 35:870-83.
8. Balech, E. 1985. The genus Alexandrium or Gonyaulax of the tamarensis group. In Anderson, D. M., White, A. W. & Baden, D. G. [Eds.] Toxic Dinoflagellates. Elsevier, New York, pp. 33-8.
9. Balech, E. 1995. The Genus Alexandrium Halim (Dinoflagellate). Sherkin Island Press, Cork, Ireland
10. Cembella, A. D. & Taylor, F. J. R. 1985. Biochemical variability within the Protogonyaulax tamarensis catenella species complex. In Anderson, D. M., White, A. W. & Baden, D. G. [Eds.] Toxic Dinoflagellates. Elsevier, New York, pp. 55-60.
11. Chen, L. C. M., Edelstein, T. & McLachlan, J. 1969. Bonnemaisonia hamifera Hariot in nature and in culture. J. Phycol. 5:211-20.
12. Giovannoni, S. J., DeLong, E. F., Olsen, G. J. & Pace, N. R. 1988. Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells. J. Bacteriol. 170:720-6.
13. Hallegraeff, G. M. 1995. Harmful algal blooms: a global overview. In Hallegraeff, G. M., Anderson, D. M. & Cembella, A. D. [Eds.] Manual on Harmful Marine Microalgae. IOC Manuals and Guides, No.33, UNESCO, Paris, pp. 1-22.
14. Heley, S. T., Cavender, J. F. & Murray, T. E. 1999. Detection of Alexandrium tamarensis by rapid PCR analysis. BioTechniques 26:88-91.
15. Miller, P. E. & Scholin, C. A. 1996. Identification of cultured Pseudnizschia (Bacillariophyceae) using species-specific LSU rRNA-targeted fluorescent probes. J. Phycol. 32:646-55.
16. Penna, A. & Magnani, M. 1999. Identification of Alexandrium (Dinophyceae) species using PCR and rDNA-targeted probes. J. Phycol. 35:615-21.
17. Penna, A. & Magnani, M. 2000. A PCR immunoassay method for the detection of Alexandrium (dinophyceae) species. J. Phycol. 36:1183-6.
18. Sako, Y. 1999. Molecular identification of toxic marine dinoflagellates Alexandrium species. Arch. Hydrobiol. 54:311-24.
19. Sako, Y., Adachi, M. & Ishida, Y. 1993. Preparation and characterization of monoclonal antibodies of Alexandrium species. In Smayda, T. J. & Shimizu, Y. [Eds.] Toxic Phytoplankton Blooms in the Sea. Elsevier, Amsterdam, pp. 87-93.
20. Sako, Y., Kim, C. H. & Ishida, Y. 1992. Mendelian inheritance of paralytic shellfish poisoning toxin in the marine dinoflagellate Alexandrium catenella. Biosci. Biotech. Biochem. 56:692-4.
21. Sako, Y., Kim, C.-H., Ninomiya, H., Adachi, M. & Ishida, Y. 1990. Isozyme and cross analysis of mating population in the Alexandrium catenella/tamarense species complex. In Graneli, E., Sundstrom, B., Edler, L. & Anderson, D. M. [Eds.] Toxic Marine Phytoplankton. Elsevier, New York, pp. 320-3.
22. Scholin, C. A. & Anderson, D. M. 1996. LSU rDNA-based RFLP assays for discriminating species and strains of Alexandrium (Dinophyceae). J. Phycol. 32:1022-35.
23. Scholin, C. A., Buck, K. R., Britschgi, T., Cangelosi, G. & Chavez, F. P. 1996. Identification of Pseud-nizschia australis (Bacillariophyceae) using rRNA-targeted probes in whole cell and sandwich hybridization formats. Phycologia 35:190-7.
24. Scholin, C. A., Herzog, M., Sogin, M. & Anderson, D. M. 1994. Identification of group and strain-specific genetic markers for globally distributed Alexandrium (Dinophyceae). II. Sequences analysis of a fragment of the LSU rRNA gene. J. Phycol. 30:999-1011.
25. Scholin, C. A., Miller, P. E., Buck, K., Chavez, F., Harris, P., Haydock, P., Howard, J. & Cangelosi, G. 1997. Detection and quantification of Pseudonizschia australis in cultured and natural populations using LSU rRNA-targeted probes. Limnol. Oceanogr. 42:1265-72.
26. Shumway, S. E. 1990. A review of the effects of algal blooms on shellfish and aquaculture. J. World Aquacult. Soc. 21:65-104.
27. Steidinger, K. A. & Moestrup, O. 1990. The taxonomy of Gonyaulax, Pyrodinium, Alexandrium, Gessnerium, Protogonyalax and Goniodoma. In Graneli, E., Sundstrom, B., Edler, L. & Anderson, D. M. [Eds.] Toxic Marine Phytoplankton. Elsevier, New York, pp. 522-3.
28. Yoshida, T., Sako, Y. & Uchida, A. 2001. Geographic differences in paralytic shellfish poisoning toxin profiles among Japanese population of Alexandrium tamarense and A. catenella (Dinophyceae). Phycol. Res. 49:13-21.