LSU rDNA-based RFLP assays for discriminating species and strains of Alexandrium (Dinophyceae)

Journal of Phycology

Volumn 32, Issue 6, 1996, Pages 1022-1035

  Scholin, Christopher A ^a   Anderson, Donald M ^b  

^a MONTEREY BAY AQUARIUM RESEARCH INSTITUTE   (United States)

^b WOODS HOLE OCEANOGRAPHIC INSTITUTION   (United States)

Author keywords

Alexandrium; biogeography; Dinophyceae; LSU rDNA; paralytic shellfish poisoning; PCR; red tide; RFLP

Indexed keywords

ALEXANDRIUM; ALEXANDRIUM ANDERSONI; ALEXANDRIUM CATENELLA; ALEXANDRIUM FUNDYENSE; ALEXANDRIUM LUSITANICUM; ALEXANDRIUM MINUTUM; ALEXANDRIUM TAMARENSE; CATENELLA; DINOPHYCEAE;

EID: 0030481827     PISSN: 00223646     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.0022-3646.1996.01022.x     Document Type: Article

References (17)

1. Adachi, M., Sako, Y. & Ishida, Y. 1994. Restriction fragment length polymorphism of ribosomal DNA internal transcribed spacer and 5.8S regions in Japanese Alexandrium species (Dinophyceae). J. Phycol. 30:857-63.
2. _ 1996. Analysis of Alexandrium (Dinophyceae) species using sequences of the 5.8S ribosomal DNA and internal transcribed spacer regions. J. Phycol. 32:424-32.
3. Anderson, D. M., Kulis, D. M., Doucette, G. J., Gallagher, J. C. & Balech, E. 1994. Biogeography of toxic dinoflagellates in the genus Alexandrium from the northeastern United States and Canada. Mar. Biol. (Berl.) 120:467-78.
4. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Siedman, J. G., Smith, J. A. & Struhl, K., Eds. 1987. Current Protocols in Molecular Biology, Vols. 1 and 2. Wiley Interscience, New York.
5. Cembella, A. D., Taylor, F. J. R. & Therriault, J-C. 1988. Cladistic analysis of electrophoretic variants within the toxic dinoflagellate genus Protogonyaulax. Bot. Mar. 31:39-51.
6. Destombe, C., Cembella, A. D., Murphy, C. A. & Ragan, M. A. 1992. Nucleotide sequence of the 18S ribosomal RNA genes form the marine dinoflagellate Alexandrium tamarense (Gonyaulacales, Dinophyta). Phycologia 31:121-4.
7. Hayhome, B. A., Anderson, D. M., Kulis, D. M. & Whitten, D. J. 1989. Variation among congeneric dinoflagellates from the northeastern United States and Canada I. Enzyme electrophoresis. Mar. Biol. (Berl.) 101:427-35.
8. Judge, B. S., Scholm, C. A. & Anderson, D. M. 1993. RFLP analysis of a fragment of the large-subunit ribosomal RNA gene of globally distributed populations of the toxic dinoflagellate Alexandrium. Biol. Bull. 185:329-30.
9. Lenaers, G., Maroteaux, L., Michot, B. & Herzog, M. 1989. Dinoflagellates in evolution. A molecular phylogenetic analysis of large subunit ribosomal RNA. J. Mol. Evol. 29:40-51.
10. Prakash, A., Medcof, J. C. & Tennant, A. D. 1971. Paralytic shellfish poisoning in eastern Canada. Bull. Fish. Res. Board Can. 177:1-87.
11. Sako, Y., Adachi, M. & Ishida, Y. 1993. Preparation and characterization of monoclonal antibodies to Alexandrium species. In Smayda, T. J. & Shimizu, Y. [Eds.] Toxic Phytoplankton Blooms In The Sea. Elsevier, New York, pp. 87-93.
12. Sako, Y., Kim, C. H., Ninomiya, H., Adachi, M. & Ishida, Y. 1990. Isozyme and cross analysis of mating populations in the Alexandrium catenella/tamarense species complex. In Graneli, E., Sundstrom, B., Edler, L. & Anderson, D. M. [Eds.] Toxic Marine Phytoplankton. Elsevier, New York, pp. 320-3.
13. Scholin, C. A. & Anderson, D. M. 1994. Identification of group-and strain-specific genetic markers for globally distributed Alexandrium (Dinophyceae). I. RFLP analysis of SSU rRNA genes. J. Phycol. 30:744-54.
14. Scholin, C. A., Anderson, D. M. & Sogin, M. 1993. The existence of two distinct small-subunit rRNA genes in the toxic dinoflagellate Alexandrium fundyense (Dinophyceae). J. Phycol. 29:209-16.
15. Scholin, C. A., Hallegraeff, G. M. & Anderson, D. M. 1995. Molecular evolution of the Alexandrium tamarense "species complex" (Dinophyceae): dispersal in the North American and West Pacific regions. Phycologia 34:472-85.
16. Scholin, C. A., Herzog, M., Sogin, M. L. & Anderson, D. M. 1994a. Identification of group and strain-specific genetic markers for globally distributed Alexandrium (Dinophyceae). II. Sequence analysis of a fragment of the LSU rRNA gene. J. Phycol. 30:999-1011.
17. Scholin, C. A., Villac, M. C., Buck, K. R., Krupp, J. M., Powers, D. A., Fryxell, G. A. & Chavez, F. P. 1994b. Ribosomal DNA sequences discriminate among toxic and non-toxic Pseudonitzschia species. Natural Toxins 2:152-65.