A solid-phase extraction/high-performance liquid chromatography-based 32P-postlabeling method for detection of cyclic 1,N 2-propanodeoxyguanosine adducts derived from enals

Analytical Biochemistry

Volumn 348, Issue 1, 2006, Pages 15-23

  Pan, Jishen ^a   Davis, Warren ^b   Trushin, Neil ^c   Amin, Shantu ^c   Nath, Raghu G ^a   Salem Jr , Norman ^d   Chung, Fung Lung ^a  

^a GEORGETOWN UNIVERSITY   (United States)

^b ROSWELL PARK CANCER INSTITUTE   (United States)

^c PENNSYLVANIA STATE UNIVERSITY   (United States)

^d NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM   (United States)

Author keywords

1,N2 propanodG; 4 Hydroxy 2 nonenal; 32P postlabeling; Acrolein; Aldehyde DNA adducts; Cyclic deoxyguanosine adducts; Enal derived cyclic adducts; High performance liquid chromatography; Solid phase extraction

Indexed keywords

ADDITION REACTIONS; ALDEHYDES; BIOMOLECULES; DNA; HERBICIDES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUIDS; PHASE SEPARATION; POLYUNSATURATED FATTY ACIDS; RATS; THIN LAYER CHROMATOGRAPHY;

1,N^2-PROPANODG; 4-HYDROXY-2-NONENAL; ACROLEIN; CYCLIC DEOXYGUANOSINE ADDUCTS; DNA ADDUCTS; ENAL-DERIVED CYCLIC ADDUCTS; SOLID-PHASE EXTRACTION; ^32P- POSTLABELING;

EXTRACTION;

4 HYDROXYNONENAL; ACROLEIN; ALKENE DERIVATIVE; CROTONALDEHYDE; DEOXYGUANOSINE DERIVATIVE; DNA; PHOSPHORUS 32;

ANALYTIC METHOD; ARTICLE; DNA MODIFICATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOTOPE LABELING; LIVER; PRIORITY JOURNAL; PURIFICATION; RING OPENING; SOLID PHASE EXTRACTION; THIN LAYER CHROMATOGRAPHY; VALIDATION PROCESS;

EID: 29144491416     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.10.011     Document Type: Article

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