Preparation of ubiquitinated substrates by the PY motif-insertion method for monitoring 26S proteasome activity

Methods in Enzymology

Volumn 399, Issue , 2005, Pages 215-227

  Saeki, Y ^a   Isono, E ^a   Toh E, A ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; UBIQUITIN; UBIQUITIN PROTEIN LIGASE;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBSTRATE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN MOTIF; PROTEIN PURIFICATION; UBIQUITINATION;

EID: 28844484999     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(05)99014-9     Document Type: Review

References (13)

1. Dunn R., Klos D.A., Adler A.S., and Hicke L. The C2 domain of the Rsp5 ubiquitin ligase binds membrane phosphoinositides and directs ubiquitination of endosomal cargo J. Cell Biol. 165 2004 135 144
2. Galan J.M., and Haguenauer-Tsapis R. Ubiquitin lys63 is involved in ubiquitination of a yeast plasma membrane protein EMBO J. 16 1997 5847 5854
3. Hershko A., and Ciechanover A. The ubiquitin system Annu. Rev. Biochem. 67 1998 425 479
4. Pickart C.M. Mechanisms underlying ubiquitination Annu. Rev. Biochem. 70 2001 503 533
5. Pickart C.M., and Cohen R.E. Proteasomes and their kin: proteases in the machine age Nat. Rev. Mol. Cell Biol. 5 2004 177 187
6. Saeki Y., Tayama Y., Toh-e A., and Yokosawa H. Definitive evidence for Ufd2-catalyzed elongation of the ubiquitin chain through Lys48 linkage Biochem. Biophys. Res. Commun. 320 2004 840 845
7. Sone T., Saeki Y., Toh-e A., and Yokosawa H. Sem1p is a novel subunit of the 26 S proteasome from Saccharomyces cerevisiae J. Biol. Chem. 279 2004 28807 28816
8. Sudol M., and Hunter T. NeW wrinkles for an old domain Cell 103 2000 1001 1004
9. Thrower J.S., Hoffman L., Rechsteiner M., and Pickart C.M. Recognition of the polyubiquitin proteolytic signal EMBO J. 19 2000 94 102
10. Verma R., Aravind L., Oania R., McDonald W.H., Yates J.R. 3rd, Koonin E.V., and Deshaies R.J. Role of Rpn11 metalloprotease in deubiquitination and degradation by the 26S proteasome Science 298 2002 611 615
11. Verma R., Chen S., Feldman R., Schieltz D., Yates J., Dohmen J., and Deshaies R.J. Proteasomal proteomics: identification of nucleotide-sensitive proteasome-interacting proteins by mass spectrometric analysis of affinity-purified proteasomes Mol. Biol. Cell 11 2000 3425 3439
12. Verma R., McDonald H., Yates J.R. 3rd, and Deshaies R.J. Selective degradation of ubiquitinated Sic1 by purified 26S proteasome yields active S phase cyclin-Cdk Mol. Cell 8 2001 439 448
13. Verma R., Oania R., Graumann J., and Deshaies R.J. Multiubiquitin chain receptors define a layer of substrate selectivity in the ubiquitin-proteasome system Cell 118 2004 99 110