Expression of a chimeric protein containing the catalytic domain of Shiga-like toxin and human granulocyte macrophage colony-stimulating factor (hGM-CSF) in Escherichia coli and its recognition by reciprocal antibodies

Iranian Biomedical Journal

Volumn 9, Issue 4, 2005, Pages 143-148

  Roudkenari, Mehryar Habibi ^a   Bouzari, Saeid ^a   Oloomi, Mana ^a   Jafari, Anis ^a   Shahrokhi, Nader ^a   Shokrgozar, Mohammad Ali ^a  

^a PASTEUR INSTITUTE OF IRAN   (Iran)

Author keywords

Chimeric protein; Escherichia coli; Expression; Human granulocyte macrophage colony stimulating factor (hGM CSF); Shiga toxin

Indexed keywords

ANTIBODY; ARABINOSE; CHIMERIC PROTEIN; GRANULOCYTE COLONY STIMULATING FACTOR; VEROTOXIN 1;

ARTICLE; ENZYME ACTIVE SITE; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE EXPRESSION; GENE FUSION; NONHUMAN; WESTERN BLOTTING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 28444447353     PISSN: 1028852X     EISSN: 1028852X     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Brinkmann, U. and Pastan, I. (1994) Immunotoxin against cancer. Biochem. Biophys. Acta 1198: 27-45.
2. O'Loughlin, E.V. and Robins-Browne, R.M. (2001) Effect of Shiga toxin and Shiga-like toxins on eukaryotic cells. Microbes Infect. 3: 493-507.
3. Sandving, K. (2001) Shiga toxins. Toxicon 39: 1629-1635.
4. Sandving, K. and Van Deurs, B. (2000) Entry of ricin and Shiga toxin into cells: Molecular mechanisms and medical perspectives. EMBO J. 19: 5943-5950.
5. Kreitman, R.J. and Pastan, I. (1997) Recombinant toxins containing human granulocyte-macrophage colony-stimulating factor and either Pseudomonas exotoxin or diphtheria toxin kill gastrointestinal cancer and leukemia cells. Blood 90: 252-259.
6. Metcalf, D. (1990) The colony stimulating factors: Discovery, development, and clinical application. Cancer 65: 2185-2195.
7. Park, L.S., Friend, D., Gillis, S. and Urdal, D.L. (1986) Characterization of the cell surface receptor for granulocyte-macrophage colony-stimulating factors. J. Biol. Chem. 261: 4177-4183.
8. rd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
9. Oloomi, M., Bouzari, S. and Rechinsky, V.O. (1998) PCR-mediated expression of the human GM-CSF in Escherichia coli. Iran. Biomed. J. 2: 21-25.
10. Frankel, A.E., Lilly, M., Kreitman, R., Hogge, D., Beran, M., Freedman, M.H., Emanuel, P.D., McLain, C., Hall, P., Tagge, E., Berger, M. and Eaves, C. (1998) Diphtheria toxin fused to granulocyte-macrophage colony-stimulating factor is toxic to blasts from patients with juvenile myelomonocytic leukemia and chronic myelomonocytic leukemia. Blood 92: 4279-44286.
11. Terpsrta, W., Rozemuller, H., Breems, D.A., Rombouts, E.J.C., Prins, A., FitzGerald, D.J.P., Kreitman, R.J., Weilenga, J.J., Poelmacher, R.E., Lowenberg, B., Hagenbeek, A. and Martens, A.C.M. (1997) Diphtheria toxin fused to granulocyte-macrophage colony-stimulating factor eliminates acute myeloid leukemia cells with the potential to initiate leukemia in immunodeficient mice, but spares normal hemopoietic stem cells. Blood 90: 3735-3742.
12. Al-Jaufy, A.Y., Haddad, J.E., King, S.R., McPhee, R.A. and Jackson, M.P. (1994) Cytotoxicity of a Shiga toxin A subunit-CD4 fusion protein to human immunodeficiency virus-infected cells. Infect. Immun. 62: 956-960.
13. Skinner, L.M. and Jackson, M.P. (1998) Inhibition of prokaryotic translation by the Shiga toxin enzymatic subunit. Microb. Pathog. 24: 117-122.
14. ctGMCSF fusion toxin in a baculovirus expression vector system for biotherapy of GMCSF-receptor positive hematologic malignancies. Protein Exp. Purif. 13: 210-221.
15. Haddad, J.E., Al-Jaufy, A.Y. and Jackson, M.P. (1993) Minimum domain of the Shiga toxin A subunit required for enzymatic activity. J. Bacteriol. 175: 4970-4978.
16. Weinstein, D.L., Jackson, M.P., Perera, L.P., Holmes, R.K. and O'Brien, D.O. (1989) In vivo formation of hybrid toxins comprising Shiga toxin and the Shiga-like toxins and role of the B subunit in localization and cytotoxic activity. Infect. Immun. 57: 3743-3750.