Three multiplex assays for detection of Borrelia burgdorferi sensu lato and Borrelia miyamotoi sensu lato in field-collected ixodes nymphs in North America

Journal of Medical Entomology

Volumn 42, Issue 6, 2005, Pages 1057-1062

  Ullmann, A J ^a   Gabitzsch, E S ^a   Schulze, T L ^b   Zeidner, N S ^a   Piesman, J ^a  

^a NATIONAL CENTER FOR EMERGING AND ZOONOTIC INFECTIOUS DISEASES   (United States)

^b Freehold Area Health Department   (United States)

Author keywords

Borrelia; Ixodes; Multiplex real time PCR

Indexed keywords

ACARI; ARTHROPODA; BORRELIA; BORRELIA ANDERSONII; BORRELIA BISSETTII; BORRELIA BURGDORFERI; BORRELIA BURGDORFERI GROUP; BORRELIA LONESTARI; BORRELIA MIYAMOTOI; IXODES; IXODES SCAPULARIS; IXODES SPINIPALPIS;

EID: 28344438798     PISSN: 00222585     EISSN: None     Source Type: Journal    
DOI: 10.1093/jmedent/42.6.1057     Document Type: Article

References (33)

1. Bacon, R. M., R. D. Gilmore, Jr., M. Quintana, J. Piesman, and B. J. Johnson. 2003. DNA evidence of Borrelia lonestari in Amblyomma americanum (Acari: Ixodidae) in southeast Missouri. J. Med. Entomol. 40: 590-592.
2. Bacon, R. M., M. A. Pilgard, B. J. Johnson, S. J. Raffel, and T. G. Schwan. 2004. Glycerophosphodiester phosphodiesterase gene (glpQ) of Borrelia lonestari identified as a target for differentiating Borrelia species associated with hard ticks (Acari: Ixodidae). J. Clin. Microbiol. 42: 2326-2328.
3. Baptista, S., A. Quaresma, T. Aires, K. Kurtenbach, M. Santos-Reis, M. Nicholson, and M. Collares-Pereira. 2004. Lyme borreliosis spirochetes in questing ticks from mainland Portugal. Int. J. Med. Microbiol. 293 Suppl. 37: 109-116.
4. Barral, M., A. L. Garcia-Perez, R. A. Juste, A. Hurtado, R. Escudero, R. E. Sellek, and P. Anda. 2002. Distribution of Borrelia burgdorferi sensu lato in Ixodes ricinus (Acari: Ixodidae) ticks from the Basque Country, Spain. J. Med. Entomol. 39: 177-184.
5. Bunikis, J., J. Tsao, C. J. Luke, M. G. Luna, D. Fish, and A. G. Barbour. 2004a. Borrelia burgdorferi infection in a natural population of Peromyscus leucopus mice: a longitudinal study in an area where Lyme Borreliosis is highly endemic. J. Infect. Dis. 189: 1515-1523.
6. Bunikis, J., J. Tsao, U. Garpmo, J. Berglund, D. Fish, and A. G. Barbour. 2004b. Typing of Borrelia relapsing fever group strains. Emerg. Infect. Dis. 10: 1661-1664.
7. Burgdorfer, W., J. F. Anderson, L. Gern, R. S. Lane, J. Piesman, and A. Spielman. 1991. Relationship of Borrelia burgdorferi to its arthropod vectors. Scand. J. Infect. Dis. Suppl. 77: 35-40.
8. Burkot, T. R., G. R. Mullen, R. Anderson, B. S. Schneider, C. M. Happ, and N. S. Zeidner. 2001. Borrelia lonestari DNA in adult Amblyomma americanum ticks, Alabama. Emerg. Infect. Dis. 7: 471-473.
9. Courtney, J. W., L. M. Kostelnik, N. S. Zeidner, and R. F. Massung. 2004. Multiplex real-time PCR for detection of Anaplasma phagocytophilum and Borrelia burgdorferi. J. Clin. Microbiol. 42: 3164-3168.
10. Dolan, M. C., J. Piesman, B. S. Schneider, M. Schriefer, K. Brandt, and N. S. Zeidner. 2004. Comparison of disseminated and nondisseminated strains of Borrelia burgdorferi sensu stricto in mice naturally infected by tick bite. Infect. Immun. 72: 5262-5266.
11. Escudero, R., M. Barral, A. Perez, M. M. Vitutia, A. L. Garcia-Perez, S. Jimenez, R. E. Sellek, and P. Anda. 2000. Molecular and pathogenic characterization of Borrelia burgdorferi sensu lato isolates from Spain. J. Clin. Microbiol. 38: 4026-4033.
12. Hanincova, K., V. Taragelova, J. Koci, S. M. Schafer, R. Hails, A. J. Ullmann, J. Piesman, M. Labuda, and K. Kurtenbach. 2003. Association of Borrelia garinii and B. valaisiana with songbirds in Slovakia. Appl. Environ. Microbiol. 69: 2825-2830.
13. Johnson, B. J., C. M. Happ, L. W. Mayer, and J. Piesman. 1992. Detection of Borrelia burgdorferi in ticks by species-specific amplification of the flagellin gene. Am. J. Trop. Med. Hyg. 47: 730-741.
14. Kurtenbach, K., M. Peacey, S. G. Rijpkema, A. N. Hoodless, P. A. Nuttall, and S. E. Randolph. 1998. Differential transmission of the genospecies of Borrelia burgdorferi sensu lato by game birds and small rodents in England. Appl. Environ. Microbiol. 64: 1169-1174.
15. Lane, R. S., J. Piesman, and W. Burgdorfer. 1991. Lyme borreliosis: relation of its causative agent to its vectors and hosts in North America and Europe. Annu. Rev. Entomol. 36: 587-609.
16. Liveris, D., G. P. Wormser, J. Nowakowski, R. Nadelman, S. Bittker, D. Cooper, S. Varde, F. H. Moy, G. Forseter, C. S. Pavia, et al. 1996. Molecular typing of Borrelia burgdorferi from Lyme disease patients by PCR-restriction fragment length polymorphism analysis. J. Clin. Microbiol. 34: 1306-1309.
17. Maupin, G. O., D. Fish, J. Zultowsky, E. G. Campos, and J. Piesman. 1991. Landscape ecology of Lyme disease in a residential area of Westchester County, New York. Am. J. Epidemiol. 133: 1105-1113.
18. Maupin, G. O., K. L. Gage, J. Piesman, J. Montenieri, S. L. Sviat, L. VanderZanden, C. M. Happ, M. Dolan, and B. J. Johnson. 1994. Discovery of an enzootic cycle of Borrelia burgdorferi in Neotoma mexicana and Ixodes spinipalpis from northern Colorado, an area where Lyme disease is nonendemic. J. Infect. Dis. 170: 636-643.
19. Montgomery, R. R., S. E. Malawista, K. J. Feen, and L. K. Bockenstedt. 1996. Direct demonstration of antigenic substitution of Borrelia burgdorferi ex vivo: exploration of the paradox of the early immune response to outer surface proteins A and C in Lyme disease. J. Exp. Med. 183: 261-269.
20. Oliver, J. H., Jr., T. M. Kollars, Jr., F. W. Chandler, Jr., A. M. James, E. J. Masters, R. S. Lane, and L. O. Huey. 1998. First isolation and cultivation of Borrelia burgdorferi sensu lato from Missouri. J. Clin. Microbiol. 36: 1-5.
21. Overbergh, L., A. Giulietti, D. Valckx, R. Decallonne, R. Bouillon, and C. Mathieu. 2003. The use of real-time reverse transcriptase PCR for the quantification of cytokine gene expression. J. Biomol. Tech. 14: 33-43.
22. Pahl, A., U. Kuhlbrandt, K. Brune, M. Rollinghoff, and A. Gessner. 1999. Quantitative detection of Borrelia burgdorferi by real-time PCR. J. Clin. Microbiol. 37: 1958-1963.
23. Peirson, S. N., J. N. Butler, and R. G. Foster. 2003. Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis. Nucleic Acids Res. 31: e73.
24. Piesman, J., T. N. Mather, J. G. Donahue, J. Levine, J. D. Campbell, S. J. Karakashian, and A. Spielman. 1986. Comparative prevalence of Babesia microti and Borrelia burgdorferi in four populations of Ixodes dammini in eastern Massachusetts. Acta Trop. 43: 263-270.
25. Postic, D., M. V. Assous, P. A. Grimont, and G. Baranton. 1994. Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons. Int. J. Syst. Bacteriol. 44: 743-752.
26. Rauter, C., R. Oehme, I. Diterich, M. Engele, and T. Hartung. 2002. Distribution of clinically relevant Borrelia genospecies in ticks assessed by a novel, single-run, real-time PCR. J. Clin. Microbiol. 40: 36-43.
27. Rijpkema, S. G., M. J. Molkenboer, L. M. Schouls, F. Jongejan, and J. F. Schellekens. 1995. Simultaneous detection and genotyping of three genomic groups of Borrelia burgdorferi sensu lato in Dutch Ixodes ricinus ticks by characterization of the amplified intergenic spacer region between 5S and 23S rRNA genes. J. Clin. Microbiol. 33: 3091-3095.
28. Scoles, G. A., M. Papero, L. Beati, and D. Fish. 2001. A relapsing fever group spirochete transmitted by Ixodes scapularis ticks. Vector Borne Zoonotic Dis. 1: 21-34.
29. Stafford, K. C., 3rd, M. L. Cartter, L. A. Magnarelli, S. H. Ertel, and P. A. Mshar. 1998. Temporal correlations between tick abundance and prevalence of ticks infected with Borrelia burgdorferi and increasing incidence of Lyme disease. J. Clin. Microbiol. 36: 1240-1244.
30. Tsao, J. I., J. T. Wootton, J. Bunikis, M. G. Luna, D. Fish, and A. G. Barbour. 2004. An ecological approach to preventing human infection: vaccinating wild mouse reservoirs intervenes in the Lyme disease cycle. Proc. Natl. Acad. Sci. U.S.A. 101: 18159-18164.
31. Wang, G., D. Liveris, B. Brei, H. Wu, R. C. Falco, D. Fish, and I. Schwartz. 2003. Real-time PCR for simultaneous detection and quantification of Borrelia burgdorferi in field-collected Ixodes scapularis ticks from the Northeastern United States. Appl. Environ. Microbiol. 69: 4561-4565.
32. Zeidner, N. S., B. S. Schneider, M. C. Dolan, and J. Piesman. 2001. An analysis of spirochete load, strain, and pathology in a model of tick-transmitted Lyme borreliosis. Vector Borne Zoonotic Dis. 1: 35-44.
33. Zeidner, N. S., T. R. Burkot, R. Massung, W. L. Nicholson, M. C. Dolan, J. S. Rutherford, B. J. Biggerstaff, and G. O. Maupin. 2000. Transmission of the agent of human granulocytic ehrlichiosis by Ixodes spinipalpis ticks: evidence of an enzootic cycle of dual infection with Borrelia burgdorferi in Northern Colorado. J. Infect. Dis. 182: 616-619.